Dynamics of the cytosolic chelatable iron pool of K562 cells.

Abstract:

:The labile iron pool of cells (LIP) constitutes the primary source of metabolic and catalytically reactive iron in the cytosol. We studied LIP homeostasis in K562 cells using the fluorescent metal-sensitive probe calcein. Following brief exposure to iron(II) salts or to oxidative or reductive stress, LIP rose by up to 120% relative to the normal level of 350nM. However, the rate of recovery to normal LIP level differed markedly with each treatment (respective t1/2s of 27, 65-88 and < or = 17 min). We show that the capacity of K562 cells to adjust LIP levels is highly dependent on the origin of the LIP increase and on the pre-existing cellular iron status.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Breuer W,Epsztejn S,Cabantchik ZI

doi

10.1016/0014-5793(96)00190-1

subject

Has Abstract

pub_date

1996-03-18 00:00:00

pages

304-8

issue

3

eissn

0014-5793

issn

1873-3468

pii

0014579396001901

journal_volume

382

pub_type

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