Dynamics of the cytosolic chelatable iron pool of K562 cells.

abstract：:Microsomal membranes from the slow-growing Morris hepatoma 9618A catalyze, in the presence of t-butyl hydroperoxide, lower rates of lipid peroxidation than rat liver microsomes. The cytochrome P-450 content of hepatoma microsomes is about 40% that of the liver. SKF 525-A, an inhibitor of mixed-function oxidase, produc...

journal_title：FEBS letters

authors： Borrello S,Galeotti T,Palombini G,Minotti G

更新日期：1986-12-15 00:00:00

abstract：:Gustatory papillae and associated taste buds receive and process chemical information from the environment. In mammals, their development takes place during the late phase of embryogenesis. However, the cellular factors that regulate the differentiation of taste papillae remain largely unknown. Here, we show by quanti...

journal_title：FEBS letters

authors： Ortíz-Alvarado R,Guzmán-Quevedo O,Mercado-Camargo R,Haertle T,Vignes C,Bolaños-Jiménez F

更新日期：2006-10-02 00:00:00

abstract：:A latent MAP kinase kinase kinase activity previously detected after chromatography of PC12 cell extracts on Mono Q [(1992) FEBS Lett. 314, 461-465] has been identified as B-Raf by immunoblotting and immunoprecipitation experiments and by its specific activation with B-Raf antibodies. B-Raf is phosphorylated after sti...

journal_title：FEBS letters

authors： Traverse S,Cohen P

更新日期：1994-08-15 00:00:00

abstract：:Previously, we cloned rat MRP3 as a candidate for an inducible transporter for the biliary excretion of organic anions [Hirohashi et al. (1998) Mol. Pharmacol. 53, 1068-10751. In the present study, we cloned human MRP3 (1527 amino acids) from Caco-2 cells. Human MRP3 is predominantly expressed in liver, small intestin...

journal_title：FEBS letters

authors： Kiuchi Y,Suzuki H,Hirohashi T,Tyson CA,Sugiyama Y

更新日期：1998-08-14 00:00:00

abstract：:Crosslinking of proteins by tissue transglutaminase (tTG) is enhanced in amyloid (Abeta) deposits characteristic of Alzheimer's disease and sporadic inclusion body myositis. Small heat shock proteins (sHsps) also occur in amyloid deposits. We here report the substrate characteristics for tTG of six sHsps. Hsp27, Hsp20...

journal_title：FEBS letters

authors： Boros S,Kamps B,Wunderink L,de Bruijn W,de Jong WW,Boelens WC

更新日期：2004-10-08 00:00:00

abstract：:Cytochrome P-450scc (P-450scc), a cholesterol side-chain cleavage enzyme from bovine adrenocortical mitochondria, has been crystallized for the first time. Upon removal of glycerol from the solution of the native enzyme complexed with pyridoxal 5'-phosphate (PLP) by microdialysis against distilled water, reddish and p...

journal_title：FEBS letters

authors： Iwamoto Y,Tsubaki M,Hiwatashi A,Ichikawa Y

更新日期：1988-06-06 00:00:00

abstract：:The chemokine RANTES is a potent chemoattractant for eosinophils. RANTES is produced by lung epithelial cells during eosinophil-rich inflammatory diseases such as asthma. In this study, we examined the effects of thiazolidinediones (TZD) on RANTES expression in a human lung epithelial cell line, A549. In A549 cells, i...

journal_title：FEBS letters

authors： Momoi A,Murao K,Imachi H,Sayo Y,Nakamura H,Hosokawa H,Sato M,Fujita J,Okada H,Ishida T,Takahara J

更新日期：1999-06-11 00:00:00

abstract：:The plant-specific GAI-RGA-and-SCR (GRAS) family of proteins function as transcriptional regulators and play critical roles in development and signalling. Recent structural studies have shed light on the molecular functions at the structural level. The conserved GRAS domain comprises an α-helical cap and α/β core subd...

journal_title：FEBS letters

authors： Hakoshima T

更新日期：2018-02-01 00:00:00

abstract：:Very long-chain fatty acids (VLCFAs) have a variety of physiological functions and are related to numerous disorders. The key step of VLCFA elongation is catalyzed by members of the elongase family, ELOVLs. Mammals have seven ELOVLs (ELOVL1-7), yet none of them has been purified and analyzed. In the presented study we...

journal_title：FEBS letters

authors： Naganuma T,Sato Y,Sassa T,Ohno Y,Kihara A

更新日期：2011-10-20 00:00:00

abstract：:Bcl-xL, a close homolog of Bcl2, is an important regulator of apoptosis and is overexpressed in human cancer. Phosphorylation of Bcl-xL can be induced by microtubule-damaging drugs such as taxol or 2-methoxyestradiol (2-ME). By site-directed mutagenesis studies, we have identified that serine 62 is the necessary site ...

journal_title：FEBS letters

authors： Basu A,Haldar S

更新日期：2003-03-13 00:00:00

abstract：:A chloroperoxidase gene from Pseudomonas pyrrocinia was cloned into Escherichia coli using the cosmid vector pJB8. The gene coding for the chloroperoxidase could be localized to a 1.5 kb fragment of DNA which was subcloned into the high-copy-number plasmid pUC18. In one subclone increased halogenating activity could b...

journal_title：FEBS letters

authors： Wolfframm C,van Pée KH,Lingens F

更新日期：1988-10-10 00:00:00

abstract：:The primary structure of nuclear factor-I (CTF/NF-I), a eukaryotic regulatory DNA-binding protein involved in both DNA replication and gene transcription, and the secondary structure predictable from it, are compared here with those of a number of prokaryotic acetylases. Hydropathy and Chou-Fasman analyses reveal that...

journal_title：FEBS letters

authors： Oikarinen J,Mannermaa RM

更新日期：1990-10-29 00:00:00

abstract：:Multidrug transporters are involved in mediating the failure of chemotherapy in treating several serious diseases. The archetypal multidrug transporter P-glycoprotein (P-gp) confers resistance to a large number of chemically and functionally unrelated anti-cancer drugs by mediating efflux from cancer cells. The abilit...

journal_title：FEBS letters

authors： Callaghan R,Ford RC,Kerr ID

更新日期：2006-02-13 00:00:00

abstract：:Fructose-1,6-bisphosphatase, a key enzyme in gluconeogenesis, undergoes catabolite inactivation when glucose is added to gluconeogenetically active cells of the yeast Saccharomyces cerevisiae. Phosphorylation of the enzyme is followed by rapid degradation. To elucidate the cellular proteolytic system involved in catab...

journal_title：FEBS letters

authors： Schork SM,Bee G,Thumm M,Wolf DH

更新日期：1994-08-01 00:00:00

abstract：:Antibodies have been prepared against the 2-oxoglutarate transport proteins purified from bovine heart and rat liver mitochondria. The anti-heart antiserum cross-reacts with the 2-oxoglutarate carrier (OGC) from beef, pig, rat and rabbit heart, but not with the OGC from liver of the same animals. Conversely, the anti-...

journal_title：FEBS letters

authors： Zara V,De Benedittis R,Ragan CI,Palmieri F

更新日期：1990-04-24 00:00:00

abstract：:Measurements of the effects of NH3/NH4+ on glucose-induced electrical activity in beta-cells from microdissected mouse islets of Langerhans and on intracellular pH in single collagenase-isolated islets pre-loaded with a fluorescent pH probe were performed and are reported here. Application of NH3/NH4+ (15 mM) in the p...

journal_title：FEBS letters

authors： Rosario LM,Rojas E

更新日期：1986-05-05 00:00:00

abstract：:Octopus, squid and cuttle-fish organs were examined for alcohol dehydrogenase activity. Only one form was detectable, with properties typical of mammalian class III alcohol dehydrogenase. The corresponding protein was purified from octopus and enzymatically characterized. Ion-exchange and affinity chromatography produ...

journal_title：FEBS letters

authors： Rosario Fernández M,Jörnvall H,Moreno A,Kaiser R,Parés X

更新日期：1993-08-16 00:00:00

abstract：:Epiregulin (EPR), a novel member of epidermal growth factor (EGF) family, is a ligand for ErbB-1 and ErbB-4 receptors. The binding affinity of EPR for the receptors is lower than those of other EGF-family ligands. The solution structure of EPR was determined using two-dimensional nuclear magnetic resonance spectroscop...

journal_title：FEBS letters

authors： Sato K,Nakamura T,Mizuguchi M,Miura K,Tada M,Aizawa T,Gomi T,Miyamoto K,Kawano K

更新日期：2003-10-23 00:00:00

abstract：:In freshly prepared low density lipoprotein (LDL), ascorbate inhibited LDL oxidation by macrophages at the higher concentrations tested (60-100 microM). In contrast, with LDL that had been allowed to autoxidise in the refrigerator (3 degrees C) for at least 10 weeks after isolation (mildly oxidised or minimally-modifi...

journal_title：FEBS letters

authors： Stait SE,Leake DS

更新日期：1994-03-21 00:00:00

abstract：:Mesenchyme forkhead-1 (MFH-1), a winged helix/forkhead transcription factor, is expressed in developing cartilaginous tissues, kidney and arch arteries, and is essential for the normal development of the axial skeleton and aortic arch formation of mice. To investigate the possible role of MFH-1 in osteogenesis and ost...

journal_title：FEBS letters

authors： Yang XL,Matsuura H,Fu Y,Sugiyama T,Miura N

更新日期：2000-03-17 00:00:00

abstract：:The structure of the complex of Ras with the Ras-binding domain of its effector RalGDS (RGS-RBD), the first genuine Ras-effector complex, has been solved by X-ray crystallography. As with the Rap-RafRBD complex (Nasser et al., 1995), the interaction is via an inter-protein beta-sheet between the switch I region of Ras...

journal_title：FEBS letters

authors： Vetter IR,Linnemann T,Wohlgemuth S,Geyer M,Kalbitzer HR,Herrmann C,Wittinghofer A

更新日期：1999-05-21 00:00:00

abstract：:Treatment of human amniotic cells (UAC) with human interferon-alpha (Hu-IFN alpha) or phorbol myristate acetate (PMA) resulted in translocation of protein kinase C (PK-C) activity from the cytosol fraction to that of the membranes. Analysis of 32P incorporation into phospholipid fractions and studies of alterations in...

journal_title：FEBS letters

authors： Premecz G,Markovits A,Bagi G,Farkas T,Földes I

更新日期：1989-06-05 00:00:00

abstract：:The activity of S-adenosylmethionine decarboxylase (AdoMetDC) in Crithidia fasciculata was shown to be correlated to the growth of the parasite. An increase in activity was observed during exponential growth. Inhibition of protein synthesis induced an extremely rapid decay of AdoMetDC activity. The half-life of the en...

journal_title：FEBS letters

authors： Nasizadeh S,Persson L

更新日期：2003-10-09 00:00:00

abstract：:Cytosolic sialidase Neu2 has been implicated in myoblast differentiation. Here we observed a significant upregulation of Neu2 expression during differentiation of murine C2C12 myoblasts. This was evidenced both as an increase in Neu2 mRNA steady-state levels and in the cytosolic sialidase enzymatic activity. To unders...

journal_title：FEBS letters

authors： Fanzani A,Giuliani R,Colombo F,Zizioli D,Presta M,Preti A,Marchesini S

更新日期：2003-07-17 00:00:00

abstract：:MRL mice bearing the lpr (Fas) or gld (Fas ligand) mutation, MRL-Fas(lpr) or MRL-FasL(gld), respectively, develop arthritis similar to rheumatoid arthritis, but C3H and C57BL/6 mice bearing such mutations do not. In MRL-Fas(lpr) mice, agalactosylated oligosaccharides in serum IgG increase significantly in comparison t...

journal_title：FEBS letters

authors： Kuroda Y,Nakata M,Nose M,Kojima N,Mizuochi T

更新日期：2001-10-26 00:00:00

abstract：:The role of protein kinase C (PKC) and p42(MAP kinase) signaling in the regulation of proliferation and apoptosis was investigated in freshly isolated and primary cultured rat hepatocytes. Acute treatment of freshly isolated hepatocytes with phenylephrine and EGF caused rapid phasic activations of p42(MAP kinase) and ...

journal_title：FEBS letters

authors： Jarvis WD,Auer KL,Spector M,Kunos G,Grant S,Hylemon P,Mikkelsen R,Dent P

更新日期：1997-07-21 00:00:00

abstract：:Cyclic ADP-ribose (cADPR), an endogenous NAD+ metabolite in many mammalian and invertebrate tissues, is a potent mediator of calcium mobilization in sea urchin eggs. Our results show that cADPR also stimulates calcium release from rat brain microsomes, marked release occurring over the concentration range 10-250 nM. T...

journal_title：FEBS letters

authors： White AM,Watson SP,Galione A

更新日期：1993-03-08 00:00:00

abstract：:The bacteriophytochrome Agp1 was reconstituted with a locked 5Zs-biliverdin in which the C(4)=C(5) and C(5)-C(6) bonds of the methine bridge between rings A and B are fixed in the Z and syn configuration/conformation, respectively. In Agp1-5Zs the photoconversion proceeds via the Lumi-R intermediate to Meta-R(A), but ...

journal_title：FEBS letters

authors： Seibeck S,Borucki B,Otto H,Inomata K,Khawn H,Kinoshita H,Michael N,Lamparter T,Heyn MP

更新日期：2007-11-27 00:00:00

abstract：:Extraction and identification of the non-covalently bound heme groups from crude membrane preparations of photoheterotrophically grown Synechocystis sp. PCC 6803 by reversed phase high performance liquid chromatography and optical spectrophotometry led to the detection of heme O in addition to hemes B and A which latt...

journal_title：FEBS letters

authors： Peschek GA,Wastyn M,Fromwald S,Mayer B

更新日期：1995-09-04 00:00:00

abstract：:In fibrous connective tissues, fibroblasts are organized into syncytia, cellular networks that enable matrix remodeling and that are interconnected by intercellular adherens junctions (AJs). The AJs of fibroblasts are mediated by N-cadherin, a broadly expressed classical cadherin that is critically involved in develop...

journal_title：FEBS letters

authors： El Sayegh TY,Kapus A,McCulloch CA

更新日期：2007-01-23 00:00:00