Dynamics of the cytosolic chelatable iron pool of K562 cells.


:The labile iron pool of cells (LIP) constitutes the primary source of metabolic and catalytically reactive iron in the cytosol. We studied LIP homeostasis in K562 cells using the fluorescent metal-sensitive probe calcein. Following brief exposure to iron(II) salts or to oxidative or reductive stress, LIP rose by up to 120% relative to the normal level of 350nM. However, the rate of recovery to normal LIP level differed markedly with each treatment (respective t1/2s of 27, 65-88 and < or = 17 min). We show that the capacity of K562 cells to adjust LIP levels is highly dependent on the origin of the LIP increase and on the pre-existing cellular iron status.




FEBS letters


Breuer W,Epsztejn S,Cabantchik ZI




Has Abstract


1996-03-18 00:00:00














  • F-actin assembly in Dictyostelium cell locomotion and shape oscillations propagates as a self-organized reaction-diffusion wave.

    abstract::The crawling locomotion and shape of eukaryotic cells have been associated with the stochastic molecular dynamics of actin and its protein regulators, chiefly Arp2/3 and Rho family GTPases, in making a cytoskeleton meshwork within cell extensions. However, the cell's actin-dependent oscillatory shape and extension dyn...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vicker MG

    更新日期:2002-01-02 00:00:00

  • Persistent activity modification of phosphatidate phosphohydrolase and fatty acyl-CoA synthetase on incubation of adipocytes with the tumour promoter 12-O-tetradecanoylphorbol 13-acetate.

    abstract::Incubation of rat adipocytes with the phorbol ester TPA caused a dose-dependent, persistent increase in phosphatidate phosphohydrolase activity and decrease in fatty acyl-CoA synthetase activity. Half-maximal effects were obtained with 1-2 X 10(-10) M TPA. TPA did not alter basal or noradrenaline-stimulated lipolysis....

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hall M,Taylor SJ,Saggerson ED

    更新日期:1985-01-07 00:00:00

  • Neurotrophin-7: a novel member of the neurotrophin family from the zebrafish.

    abstract::A novel member of the neurotrophin family, zebrafish neurotrophin-7 (zNT-7), was isolated from the zebrafish Danio rerio. The amino acid sequence of zNT-7 is more closely related to that of fish nerve growth factor (NGF) and neurotrophin-6 (NT-6) than to that of any other neurotrophin. zNT-7 is, however, equally relat...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nilsson AS,Fainzilber M,Falck P,Ibáñez CF

    更新日期:1998-03-13 00:00:00

  • Reversible inactivation of endothelial nitric oxide synthase by NG-nitro-L-arginine.

    abstract::NG-Methyl-L-arginine (L-NMA) and NG-nitro-L-arginine (L-NNA) inhibited NO-induced cGMP accumulation in porcine aortic endothelial cells with half-maximally effective concentrations of 15 and 3.4 microM, respectively. The effects of both compounds were reversible, but the L-NNA-induced inhibition was only reversed by w...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mayer B,Schmid M,Klatt P,Schmidt K

    更新日期:1993-10-25 00:00:00

  • Transgenic UCP1 in white adipocytes modulates mitochondrial membrane potential.

    abstract::To test if mitochondrial uncoupling in white adipocytes is responsible for obesity resistance of the aP2-Ucp transgenic mice expressing ectopic uncoupling protein 1 (UCPI) in white fat, mitochondrial membrane potential (delta psi(m)) was estimated by flow cytometry in adipocytes isolated from gonadal fat. Ectopic UCP1...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Baumruk F,Flachs P,Horáková M,Floryk D,Kopecký J

    更新日期:1999-02-12 00:00:00

  • DNMT1 cooperates with MBD4 to inhibit the expression of Glucocorticoid-induced TNFR-related protein in human T cells.

    abstract::Glucocorticoid-induced TNFR-related protein (GITR) is constitutively expressed in T regulatory (Treg) cells and regulates their suppressive function. We identified two methylated CpG islands in the Gitr locus. Using a ChIP assay, we demonstrate that both DNMT1 and methyl-CpG-binding domain Protein 4 (MBD4) bind to the...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Wang S,Li Y,Zhu F,Lin F,Luo X,Zhao B,Zhang P,Li D,Gao Y,Liang R,Liu L,Tsun A,Yuan X,Wu K,Li B

    更新日期:2017-07-01 00:00:00

  • Cytoplasmic pH, a key determinant of growth factor-induced DNA synthesis in quiescent fibroblasts.

    abstract::In response to growth factors, quiescent fibroblast mutants lacking Na+/H+ exchange activity fail to elevate their cytoplasmic pH (pHi) and to reinitiate DNA synthesis at neutral and acidic pHo. A pHi threshold of approximately 7.2 exists, below which growth factors cannot set in motion the Go to S phase transition. R...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pouysségur J,Franchi A,L'Allemain G,Paris S

    更新日期:1985-10-07 00:00:00

  • Purification of a putative Na+/D-glucose cotransporter from pig kidney brush border membranes on a phlorizin affinity column.

    abstract::Phlorizin, a potent inhibitor of the Na+/D-glucose cotransporter, was derivatised to 3-aminophlorizin and subsequently coupled to Affi-Gel 15. Affinity chromatography of pig kidney brush border membranes solubilised in Triton X-100 allowed the purification of a 60 kDa protein on this resin. We consider this protein to...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kitlar T,Morrison AI,Kinne R,Deutscher J

    更新日期:1988-07-04 00:00:00

  • Caenorhabditis elegans small heat-shock proteins Hsp12.2 and Hsp12.3 form tetramers and have no chaperone-like activity.

    abstract::Four 12.2-12.6 kDa small heat-shock proteins (sHSPs) of Caenorhabditis elegans are the smallest known members of the sHSP family. They essentially comprise the characteristic C-terminal 'alpha-crystallin domain' of the sHSPs, having a very short N-terminal region, and lacking a C-terminal tail. Recombinant Hsp12.2 and...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kokke BP,Leroux MR,Candido EP,Boelens WC,de Jong WW

    更新日期:1998-08-21 00:00:00

  • Thermodynamic and kinetic control of ATP synthesis in yeast mitochondria: role of delta pH.

    abstract::ATP synthesis rate, measured as the variation in external Pi concentration, varied as a linear function of either delta mu H+ or delta Gpin, in such a manner that the delta Gpin/delta mu H+ ratio increased while VATP increased. We also observed a linear dependence of the flux control coefficient of the Pi carrier on...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Beauvoit B,Rigoulet M,Guerin B

    更新日期:1989-02-27 00:00:00

  • F-box protein FBXL2 inhibits gastric cancer proliferation by ubiquitin-mediated degradation of forkhead box M1.

    abstract::F-box/LRR-repeat protein 2 (FBXL2), a component of Skp-Cullin-F box (SCF) ubiquitin E3 ligase, has been shown to inhibit tumorigenesis by targeting and ubiquitinating several oncoproteins. However, its role in gastric cancer remains poorly understood. Here, by tandem mass spectrometry, we show that FBXL2 interacts wit...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Li LQ,Pan D,Chen H,Zhang L,Xie WJ

    更新日期:2016-02-01 00:00:00

  • Ascorbic acid accumulation in plated human neutrophils.

    abstract::Ascorbic acid uptake was investigated in isolated, plated human neutrophils using high-performance liquid chromatography with coulometric electrochemical detection. Freshly isolated neutrophils contained 1.3 mM ascorbic acid and accumulated significantly greater amounts when physiologic concentrations of the vitamin w...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Washko P,Rotrosen D,Levine M

    更新日期:1990-01-15 00:00:00

  • Growth factor protection against cytokine-induced apoptosis in neonatal rat islets of Langerhans: role of Fas.

    abstract::Treatment of neonatal rat islets of Langerhans with combined cytokines (interleukin-1beta 10(-10) M, tumour necrosis factor-alpha 10(-10) M, interferon-gamma 5 U/ml) led to extensive cell death, which was potentiated by Fas activation with the anti-Fas cytolytic antibody JO2. Pre-treatment with insulin (25 ng/ml) or i...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Harrison M,Dunger AM,Berg S,Mabley J,John N,Green MH,Green IC

    更新日期:1998-09-18 00:00:00

  • The cell membrane-shielding function of eicosapentaenoic acid for Escherichia coli against exogenously added hydrogen peroxide.

    abstract::The colony-forming ability of catalase-deficient Escherichia coli mutant genetically modified to produce eicosapentaenoic acid (EPA) showed less decrease than in a control strain producing no EPA, when treated with 0.3mM hydrogen peroxide (H(2)O(2)) under non-growth conditions. H(2)O(2)-induced protein carbonylation w...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nishida T,Orikasa Y,Watanabe K,Okuyama H

    更新日期:2006-12-11 00:00:00

  • Mitochondrial uncoupling proteins and phylogenesis--UCP4 as the ancestral uncoupling protein.

    abstract::We searched for the previously defined uncoupling protein (UCP) signatures [Jezek, P. and Urbánková, E. (2000) IUBMB Life 49, 63-70] in genomes of Drosophila melanogaster, Caenorhabditis elegans, Dictyostelium discoideum, and Arabidopsis thaliana. We identified four UCPs in Drosophila and one in Caenorhabditis or Dict...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hanák P,Jezek P

    更新日期:2001-04-27 00:00:00

  • The pro sequence of lactase-phlorizin hydrolase is required for the enzyme to reach the plasma membrane. An intramolecular chaperone?

    abstract::Various cDNAs coding for part or all of human pre-pro lactase-phlorizin hydrolase (pre-proLPH) were transfected into COS cells and the subcellular location of the lactase-related proteins assessed. Only the complete proLPH reached the plasma membrane. LPH without the pro sequence, and a construct containing the pro se...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Oberholzer T,Mantei N,Semenza G

    更新日期:1993-10-25 00:00:00

  • The activity of cAMP-phosphodiesterase 4D7 (PDE4D7) is regulated by protein kinase A-dependent phosphorylation within its unique N-terminus.

    abstract::The cyclic AMP phosphodiesterases type 4 (PDE4s) are expressed in a cell specific manner, with intracellular targeting directed by unique N-terminal anchor domains. All long form PDE4s are phosphorylated and activated by PKA phosphorylation within their upstream conserved region 1 (UCR1). Here, we identify and charact...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Byrne AM,Elliott C,Hoffmann R,Baillie GS

    更新日期:2015-03-12 00:00:00

  • Effects of analogues of adenosine and methyl xanthines on insulin sensitivity in soleus muscle of the rat.

    abstract::The concentration of insulin that produces half-maximal stimulation of glycolysis by stripped soleus muscle preparations is markedly increased by the adenosine analogues, 2-chloroadenosine and N6-phenylisopropyladenosine, but is markedly decreased by the methyl xanthine analogue, 8-phenyltheophylline. 2-Chloroadenosin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Budohoski L,Challiss RA,McManus B,Newsholme EA

    更新日期:1984-02-13 00:00:00

  • Localization of myosin IIB at the leading edge of growth cones from rat dorsal root ganglionic cells.

    abstract::Primary cultures of rat dorsal root ganglionic (DRG) cells were stained with isoform-specific antibodies against non-muscle myosin II. Antibodies against the brain type myosin (MIIB) stained the peripheries of growth cones and non-neuronal cells. Double staining of the cells with the anti-myosin antibodies and rhodami...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cheng TP,Murakami N,Elzinga M

    更新日期:1992-10-19 00:00:00

  • Crystal structure of metagenome-derived LC9-RNase H1 with atypical DEDN active site motif.

    abstract::The crystal structure of metagenome-derived LC9-RNase H1 was determined. The structure-based mutational analyses indicated that the active site motif of LC9-RNase H1 is altered from DEDD to DEDN. In this motif, the location of the second glutamate residue is moved from αA-helix to β1-strand immediately next to the fir...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nguyen TN,You DJ,Kanaya E,Koga Y,Kanaya S

    更新日期:2013-05-02 00:00:00

  • A novel immunoassay with direct relevance to protection against organophosphate poisoning.

    abstract::Antiparaoxon immune sera were employed in a new immunoassay based on competition between acetylcholinesterase and antibodies for the binding of paraoxon. Unlike radioimmunoassay, the new assay described herein can be extended to predict the feasibility of antibodies to confer in vivo protection of acetylcholinesterase...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Heldman E,Balan A,Horowitz O,Ben-Zion S,Torten M

    更新日期:1985-01-28 00:00:00

  • RNase L facilitates the repair of DNA double-strand breaks through the nonhomologous end-joining pathway.

    abstract::RNA molecules have been found to play important roles in DNA double-strand break (DSB) repair, but the exact underlying mechanism remains unclear. Here, we aimed to clarify the function of RNase L, an important ribonuclease in the immune system of vertebrates, in DSB repair. Knockdown of RNase L reduces cell survival ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zhong Y,Pan B,Zhu J,Fu H,Zheng X

    更新日期:2019-06-01 00:00:00

  • tRNAs: cellular barcodes for amino acids.

    abstract::The role of tRNA in translating the genetic code has received considerable attention over the last 50 years, and we now know in great detail how particular amino acids are specifically selected and brought to the ribosome in response to the corresponding mRNA codon. Over the same period, it has also become increasingl...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Banerjee R,Chen S,Dare K,Gilreath M,Praetorius-Ibba M,Raina M,Reynolds NM,Rogers T,Roy H,Yadavalli SS,Ibba M

    更新日期:2010-01-21 00:00:00

  • Analysis of gene expression in rose petals using expressed sequence tags.

    abstract::Single-pass sequences were obtained from the 5'-ends of a total of 1794 rose petal cDNA clones. Cluster analysis identified 242 groups of sequences and 635 singletons indicating that the database represents a total of 877 genes. Putative functions could be assigned to 1151 of the transcripts. Expression analysis indic...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Channelière S,Rivière S,Scalliet G,Szecsi J,Jullien F,Dolle C,Vergne P,Dumas C,Bendahmane M,Hugueney P,Cock JM

    更新日期:2002-03-27 00:00:00

  • cDNA encoding a 59 kDa homolog of ribosomal protein S6 kinase from rabbit liver.

    abstract::We have isolated cDNA molecules encoding a protein with the characteristic sequence elements that are conserved between the catalytic domains of protein kinases. This protein is apparently a serine/threonine kinase and is most closely related to the amino-terminal half of the ribosomal protein S6 kinase II first chara...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Harmann B,Kilimann MW

    更新日期:1990-10-29 00:00:00

  • 1H NMR studies of the paramagnetic CuA center of cytochrome oxidase.

    abstract::The dinuclear paramagnetic center of the soluble CuA domain of the cytochrome c oxidase from Bacillus subtilis has been studied using 1H NMR. The spectrum possesses remarkably sharp shifted resonances. Comparison with the spectrum of the CuA amicyanin variant provides the spin density distribution in the CuA site of c...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dennison C,Berg A,de Vries S,Canters GW

    更新日期:1996-10-07 00:00:00

  • A novel plant nuclear gene encoding chloroplast ribosomal protein S9 has a transit peptide related to that of rice chloroplast ribosomal protein L12.

    abstract::We have cloned a novel nuclear gene for a ribosomal protein of rice and Arabidopsis that is like the bacterial ribosomal protein S9. To determine the subcellular localization of the gene product, we fused the N-terminal region and green fluorescent protein and expressed it transiently in rice seedlings. Localized fluo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Arimura S,Takusagawa S,Hatano S,Nakazono M,Hirai A,Tsutsumi N

    更新日期:1999-05-07 00:00:00

  • Effect of modification of carbohydrate component on properties of glucoamylase.

    abstract::In this study, we investigated enzymatic deglycosylation of glucoamylase from Aspergillus awamori X 100/D27, a glycoprotein which has two N-linked and about forty short mannose-bearing O-linked sugars per molecule. O-Linked sugars were modified by treatment with alpha-mannosidase and N-linked sugars were removed using...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Neustroev KN,Golubev AM,Firsov LM,Ibatullin FM,Protasevich II,Makarov AA

    更新日期:1993-01-25 00:00:00

  • Rapidly maturing red fluorescent protein variants with strongly enhanced brightness in bacteria.

    abstract::A rapidly maturing variant of the red fluorescent protein DsRed was optimized for bacterial expression by random mutagenesis. The brightest variant contains six mutations, two of which (S4T and a silent mutation in codon 2) explain most of the fluorescence enhancement. The novel variants are expressed at 9-60-fold hig...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sörensen M,Lippuner C,Kaiser T,Misslitz A,Aebischer T,Bumann D

    更新日期:2003-09-25 00:00:00

  • Binding of progastrin fragments to the 78 kDa gastrin-binding protein.

    abstract::The non-selective gastrin/cholecystokinin receptor antagonists proglumide and benzotript inhibit colon carcinoma cell proliferation by binding to the 78 kDa gastrin-binding protein (GBP) (Baldwin, Proc. Natl. Acad. Sci. USA, 91 (1994) 7593-7597). However, although most colon carcinoma cell lines synthesize progastrin,...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Baldwin GS

    更新日期:1995-02-06 00:00:00