Abstract:
:The labile iron pool of cells (LIP) constitutes the primary source of metabolic and catalytically reactive iron in the cytosol. We studied LIP homeostasis in K562 cells using the fluorescent metal-sensitive probe calcein. Following brief exposure to iron(II) salts or to oxidative or reductive stress, LIP rose by up to 120% relative to the normal level of 350nM. However, the rate of recovery to normal LIP level differed markedly with each treatment (respective t1/2s of 27, 65-88 and < or = 17 min). We show that the capacity of K562 cells to adjust LIP levels is highly dependent on the origin of the LIP increase and on the pre-existing cellular iron status.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Breuer W,Epsztejn S,Cabantchik ZIdoi
10.1016/0014-5793(96)00190-1subject
Has Abstractpub_date
1996-03-18 00:00:00pages
304-8issue
3eissn
0014-5793issn
1873-3468pii
0014579396001901journal_volume
382pub_type
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