Dynamics of the cytosolic chelatable iron pool of K562 cells.


:The labile iron pool of cells (LIP) constitutes the primary source of metabolic and catalytically reactive iron in the cytosol. We studied LIP homeostasis in K562 cells using the fluorescent metal-sensitive probe calcein. Following brief exposure to iron(II) salts or to oxidative or reductive stress, LIP rose by up to 120% relative to the normal level of 350nM. However, the rate of recovery to normal LIP level differed markedly with each treatment (respective t1/2s of 27, 65-88 and < or = 17 min). We show that the capacity of K562 cells to adjust LIP levels is highly dependent on the origin of the LIP increase and on the pre-existing cellular iron status.




FEBS letters


Breuer W,Epsztejn S,Cabantchik ZI




Has Abstract


1996-03-18 00:00:00














  • Characterization of a 41 kDa photoinhibition adduct in isolated photosystem II reaction centres.

    abstract::When isolated reaction centres of photosystem II are subjected to photoinhibitory illumination, a 41 kDa SDS-PAGE band is observed under all experimental conditions. The same band is also found, together with lower molecular weight fragments of the D1 protein, in whole thylakoids and in all PSII sub-particles investig...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Barbato R,Friso G,Rigoni F,Frizzo A,Giacometti GM

    更新日期:1992-09-07 00:00:00

  • Na,K-ATPase labelled with 5-iodoacetamidofluorescein: E2-E1 conformational transition induced by different nucleotides.

    abstract::A conformational transition between E2 and E1 forms of Na, K-ATPase induced by different nucleotides has been studied under steady state conditions using the enzyme labelled with 5-iodoacetamidofluorescein. In the presence of K+ the plot of fluorescence as a function of [ATP], [ADP] or [CTP] (in a range of 5 microM-12...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lopina OD,Pindel EV,Boldyrev AA

    更新日期:1990-06-18 00:00:00

  • Efficient CD4 binding and immunosuppressive properties of the 13B8.2 monoclonal antibody are displayed by its CDR-H1-derived peptide CB1.

    abstract::A systematic exploration of the V(H)2/V(kappa)12-13 variable domains of the anti-CD4 monoclonal antibody (mAb) 13B8.2 was performed by the Spot method to screen for paratope-derived peptides (PDPs) demonstrating CD4 binding ability. Nine peptides, named CB1 to CB9, were identified, synthesized in a cyclic and soluble ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bès C,Briant-Longuet L,Cerruti M,De Berardinis P,Devauchelle G,Devaux C,Granier C,Chardès T

    更新日期:2001-11-09 00:00:00

  • Voltage-gated K+ currents of mouse dendritic cells.

    abstract::Dendritic cells (DC) were enriched from murine spleen by exploring their intermediate density and transient weak adherence. The isolated population contained excellent antigen presenting cells with high surface expression of major histocompatibility complex (MHC) class II determinants thus exhibiting crucial immunofun...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fischer HG,Eder C

    更新日期:1995-10-09 00:00:00

  • Cloning of boar SPMI gene which is expressed specifically in seminal vesicle and codes for a sperm motility inhibitor protein.

    abstract::Boar semen contains a seminal plasma motility inhibitor (SPMI) that blocks the motility of demembranated-reactivated spermatozoa as well as of intact spermatozoa. In this paper, we describe the primary structure of SPMI, the coding of boar SPMI cDNA gene and its expression in various porcine tissues. Nucleotide sequen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Iwamoto T,Hiroaki H,Furuichi Y,Wada K,Satoh M,Satoh M,Osada T,Gagnon C

    更新日期:1995-07-24 00:00:00

  • Counter-regulatory paracrine actions of FGF-23 and 1,25(OH)2 D in macrophages.

    abstract::Mechanisms underlying the association between fibroblastic growth factor 23 (FGF-23) and inflammation are uncertain. We found that FGF-23 was markedly up-regulated in LPS/INF-γ-induced proinflammatory M1 macrophages and Hyp mouse-derived peritoneal macrophages, but not in IL-4-induced M2 anti-inflammatory macrophages....

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Han X,Li L,Yang J,King G,Xiao Z,Quarles LD

    更新日期:2016-01-01 00:00:00

  • The induction of early response genes in rat smooth muscle cells by PDGF-AA is not sufficient to stimulate DNA-synthesis.

    abstract::The effect of the three platelet-derived growth factor (PDGF) isoforms AA, AB and BB on the induction of the early growth response genes c-fos, egr-1 and c-myc mRNA in vascular smooth muscle cells from rat was compared with their respective mitogenic potency. The three PDGF isoforms strongly stimulated the induction t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sachinidis A,Schulte K,Ko Y,Meyer zu Brickwedde MK,Hoppe V,Hoppe J,Vetter H

    更新日期:1993-03-22 00:00:00

  • Identification of the axial heme ligands of cytochrome b556 in succinate: ubiquinone oxidoreductase from Escherichia coli.

    abstract::Electron paramagnetic resonance (EPR) and near-infrared magnetic circular dichroism (MCD) have been used to identify the ligands to the cytochrome b556 component of succinate: ubiquinone oxidoreductase (succinate dehydrogenase) from Escherichia coli. The 'highly axial low spin' (HALS) EPR spectrum suggests bis(histidi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Peterson J,Vibat C,Gennis RB

    更新日期:1994-11-28 00:00:00

  • Role of thioredoxin-1 in apoptosis induction by alpha-tocopheryl succinate and TNF-related apoptosis-inducing ligand in mesothelioma cells.

    abstract::Malignant mesothelioma (MM) is a fatal type of cancer. We studied the role of the redox-active protein thioredoxin-1 (Trx-1) in apoptosis induced in MM cells and their non-malignant counterparts (Met-5A) by alpha-tocopheryl succinate (alpha-TOS) and TNF-related apoptosis-inducing ligand (TRAIL). MM cells were suscepti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Freeman RE,Neuzil J

    更新日期:2006-05-15 00:00:00

  • Identification and differential regional expression of KOR-3/ORL-1 gene splice variants in mouse brain.

    abstract::KOR-3, also known as ORL-1, is a member of the opioid receptor family, encoding the murine receptor for orphanin FQ/nociceptin. In the current studies we have identified five different splice variants of KOR-3 in mouse brain, three of which have not been previously reported. In addition to variants with a 15 bp deleti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pan YX,Xu J,Wan BL,Zuckerman A,Pasternak GW

    更新日期:1998-09-11 00:00:00

  • Amylin injection causes elevated plasma lactate and glucose in the rat.

    abstract::Intravenous injections of 25.5 nmol rat amylin into fasted anesthetized rats caused a rapid increase in plasma lactate followed by an increase in plasma glucose; there was a transient fall in blood pressure. Subcutaneous injection of 25.5 nmol amylin also caused increases in lactate and glucose but did not change bloo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Young AA,Wang MW,Cooper GJ

    更新日期:1991-10-07 00:00:00

  • EPR detection of phytophenoxyl radicals stabilized by zinc ions: evidence for the redox coupling of plant phenolics with ascorbate in the H2O2-peroxidase system.

    abstract::Chlorogenic acid (CGA; 3-o-caffeoylquinic acid), a phenylpropanoid metabolite of plants, was oxidized by H2O2 in the presence of horseradish peroxidase. The primary and secondary oxidized products both were free radicals which gave EPR multiline signals at g = 2.0044 and 2.0042 in the presence of zinc as a spin stabil...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yamasaki H,Grace SC

    更新日期:1998-02-06 00:00:00

  • VASP interaction with vinculin: a recurring theme of interactions with proline-rich motifs.

    abstract::VASP (vasodilator-stimulated phosphoprotein), a protein associated with microfilaments at cellular contact sites, has been identified as a ligand for profilin and zyxin, two proteins also involved in microfilament dynamics and organization at these regions. Here, we report that VASP also directly binds to vinculin, an...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Reinhard M,Rüdiger M,Jockusch BM,Walter U

    更新日期:1996-12-09 00:00:00

  • Patch-clamp study of liver nuclear ionic channels reconstituted into giant proteoliposomes.

    abstract::Nuclear ionic channels (NICs) represent ubiquitous structures of living cells, although little is known about their functional properties and encoding genes. To characterize NICs, liver nuclear membrane vesicles were reconstituted into either planar lipid bilayers or proteoliposomes. Reconstitution of nuclear envelope...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Guihard G,Proteau S,Payet MD,Escande D,Rousseau E

    更新日期:2000-07-07 00:00:00

  • Thr90 is a key residue of the bacteriorhodopsin proton pumping mechanism.

    abstract::Mutation of Thr90 to Ala has a profound effect on bacteriorhodopsin properties. T90A shows about 20% of the proton pumping efficiency of wild type, once reconstituted into liposomes. Mutation of Thr90 influences greatly the Schiff base/Asp85 environment, as demonstrated by altered lambda(max) of 555 nm and pK(a) of As...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Perálvarez A,Barnadas R,Sabés M,Querol E,Padrós E

    更新日期:2001-11-23 00:00:00

  • Apolipoprotein E protects astrocytes from hypoxia and glutamate-induced apoptosis.

    abstract::Apolipoprotein E (apoE) is predominantly synthesized by astrocytes in the brain. In this study, we investigated the role of apoE in astrocyte apoptosis. We demonstrated that apoE protects astrocytes from hypoxia-induced apoptosis in a dose-dependent manner. Glutamate release from astrocytic cultures is significantly l...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zhou S,Wu H,Zeng C,Xiong X,Tang S,Tang Z,Sun X

    更新日期:2013-01-16 00:00:00

  • Chronic lung inflammation in aging mice.

    abstract::To determine whether aging is associated with a pro-inflammatory shift in the lung, inflammation and inflammation-related gene expression in the lungs of 12-week-old and 24-month-old Balb/c mice were studied. cDNA microarray and quantitative reverse transcription-polymerase chain reaction analyses showed that eight in...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Aoshiba K,Nagai A

    更新日期:2007-07-24 00:00:00

  • The yeast metacaspase is implicated in oxidative stress response in frataxin-deficient cells.

    abstract::Friedreich ataxia is the most common recessive neurodegenerative disease and is caused by reduced expression of mitochondrial frataxin. Frataxin depletion causes impairment in iron-sulfur cluster and heme biosynthesis, disruption of iron homeostasis and hypersensitivity to oxidants. Currently no pharmacological treatm...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lefevre S,Sliwa D,Auchère F,Brossas C,Ruckenstuhl C,Boggetto N,Lesuisse E,Madeo F,Camadro JM,Santos R

    更新日期:2012-01-20 00:00:00

  • The directionality of processive enzymes acting on recalcitrant polysaccharides is reflected in the kinetic signatures of oligomer degradation.

    abstract::The enzymatic degradation of the closely related insoluble polysaccharides; cellulose (β(1-4)-linked glucose) by cellulases and chitin (β(1-4)-linked N-acetylglucosamine) by chitinases, is of large biological and economical importance. Processive enzymes with different inherent directionalities, i.e. attacking the pol...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hamre AG,Schaupp D,Eijsink VG,Sørlie M

    更新日期:2015-07-08 00:00:00

  • Using lipoate enantiomers and thioredoxin to study the mechanism of the 2-oxoacid-dependent dihydrolipoate production by the 2-oxoacid dehydrogenase complexes.

    abstract::The thioredoxin-catalyzed insulin reduction by dihydrolipoate was applied to study the 2-oxoacid: lipoate oxidoreductase activity of 2-oxoacid dehydrogenase complexes. The enzymatic and non-enzymatic mechanisms of the transfer of reducing equivalents from the complexes to free lipoic acid (alpha-lipoic acid, 6,8-thioo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bunik V,Shoubnikova A,Loeffelhardt S,Bisswanger H,Borbe HO,Follmann H

    更新日期:1995-09-04 00:00:00

  • Reactivity of glutaredoxins 1, 2 and 3 from Escherichia coli and protein disulfide isomerase towards glutathionyl-mixed disulfides in ribonuclease A.

    abstract::We have examined the activity of protein disulfide isomerase (PDI) and glutaredoxin (Grx) 1, 2 and 3 from Escherichia coli to catalyze the cleavage of glutathionylated ribonuclease A (RNase-SG) by 1 mM GSH to yield reduced RNase. Apparent Km values for RNase-SG were similar, 2-10 microM, for Grx 1, 3 and PDI but Grx I...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lundström-Ljung J,Vlamis-Gardikas A,Aslund F,Holmgren A

    更新日期:1999-01-25 00:00:00

  • NAD(P)H- and superoxide-dependent nitric oxide degradation by rat liver mitochondria.

    abstract::Mitochondria consume nitric oxide (NO) mainly through reaction with superoxide anion (O(2)(-)). Here, we analyzed the O(2)(-) sources for NO degradation by isolated rat liver mitochondria. Electron leakage from complex III and reverse electron transport to complex I accounted for O(2)(-)-dependent NO degradation by mi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Oliveira HC,Saviani EE,Salgado I

    更新日期:2009-07-07 00:00:00

  • Tumor suppressor RBM5 directly interacts with the DExD/H-box protein DHX15 and stimulates its helicase activity.

    abstract::RNA binding motif protein 5 (RBM5) is a candidate tumor suppressor gene. Recent studies showed that RBM5 functions as an alternative splicing regulator of apoptosis-related genes. Here, we identify DHX15 and PRP19, two spliceosome components, as novel RBM5-interacting partners. We then show that the G-patch domain of ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Niu Z,Jin W,Zhang L,Li X

    更新日期:2012-04-05 00:00:00

  • Mutations in subunit 6 of the F1F0-ATP synthase cause two entirely different diseases.

    abstract::A lowered efficiency of oxidative phosphorylation was recently found in a Leber hereditary optic neuropathy (LHON) proband carrying a mutation in the mtDNA gene for subunit 6 of the membrane-bound F0 segment of the F1F0-ATP synthase [9]. This phenotype was transferred to cytoplasmic hybrid cells together with the muta...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Majander A,Lamminen T,Juvonen V,Aula P,Nikoskelainen E,Savontaus ML,Wikström M

    更新日期:1997-07-28 00:00:00

  • The formyl peptide chemoattractant receptor is encoded by a 2 kilobase messenger RNA. Expression in Xenopus oocytes.

    abstract::Activation of the formyl peptide chemoattractant receptor (FPCR) of phagocytic cells mobilizes intracellular calcium stores and affects the plasma membrane potential. Affinity crosslinking of FPCR has demonstrated a 60-80 kDa glycoprotein, with core peptide of 32 kDa. It is not known whether functional FPCR is this si...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Murphy PM,Gallin EK,Tiffany HL,Malech HL

    更新日期:1990-02-26 00:00:00

  • 31P-NMR studies of Mycoplasma gallisepticum cells using a continuous perfusion technique.

    abstract::31P-NMR studies of Mycoplasma gallisepticum cells have been carried out using a continuous perfusion technique; these are the first such studies with this organism. Using this technique, glucose metabolism was monitored in the intact organisms, and cell extracts were prepared to identify the intermediates. Under glyco...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Egan W,Barile M,Rottem S

    更新日期:1986-08-18 00:00:00

  • Effects of substitutions of glycine and asparagine for serine132 on activity and binding of human lipoprotein lipase to very low density lipoproteins.

    abstract::For studying the role of Ser132 in the putative catalytic site of human lipoprotein lipase (LPL), mutant LPL cDNAs expressing LPLs with amino acid substitutions of Gly or Asn for Ser132 were obtained by site-directed mutagenesis, and were expressed in COS-1 cells. Considerable amounts of LPL enzyme protein mass were d...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tashiro J,Kobayashi J,Shirai K,Saito Y,Fukamachi I,Hashimoto H,Nishida T,Shibui T,Morimoto Y,Yoshida S

    更新日期:1992-02-17 00:00:00

  • Electrostatic influence of active-site waters on the nucleophilic aromatic substitution catalyzed by 4-chlorobenzoyl-CoA dehalogenase.

    abstract::The 4-chlorobenzoyl-CoA dehalogenase catalyzes the hydrolytic dechlorination of 4-chlorobenzoyl-CoA via a two-step mechanism, namely nucleophilic aromatic substitution and ester hydrolysis. The mutation of an active-site Histidine residue has been shown to reduce the catalytic activity in both the substitution and sub...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Xu D,Guo H

    更新日期:2005-08-15 00:00:00

  • Genetic code preferentially conserves long-range interactions among the amino acids.

    abstract::The physical properties of amino acids were investigated in order to evaluate their possible relationship to the assignment of codons for amino acids in the genetic code. A comparison of the interconversion probability between amino acids and the distances between the amino acids for individual physical properties rev...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sitaramam V

    更新日期:1989-04-10 00:00:00

  • Primary structure of the 5 S subunit of transcarboxylase as deduced from the genomic DNA sequence.

    abstract::Transcarboxylase from Propionibacterium shermanii is a complex biotin-containing enzyme composed of 30 polypeptides of three different types. It is composed of six dimeric outer subunits associated with a central cylindrical hexameric subunit through 12 biotinyl subunits; three outer subunits on each face of the centr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Thornton CG,Kumar GK,Shenoy BC,Haase FC,Phillips NF,Park VM,Magner WJ,Hejlik DP,Wood HG,Samols D

    更新日期:1993-09-13 00:00:00