Abstract:
:Very long-chain fatty acids (VLCFAs) have a variety of physiological functions and are related to numerous disorders. The key step of VLCFA elongation is catalyzed by members of the elongase family, ELOVLs. Mammals have seven ELOVLs (ELOVL1-7), yet none of them has been purified and analyzed. In the presented study we purified ELOVL7 and measured its activity by reconstituting it into proteoliposomes. Purified ELOVL7 exhibited high activity toward acyl-CoAs with C18 carbon chain length. The calculated K(m) values toward C18:3(n-3)-CoA and malonyl-CoA were both in the μM range. We also found that progression of the VLCFA cycle enhances ELOVL7 activity.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Naganuma T,Sato Y,Sassa T,Ohno Y,Kihara Adoi
10.1016/j.febslet.2011.09.024subject
Has Abstractpub_date
2011-10-20 00:00:00pages
3337-41issue
20eissn
0014-5793issn
1873-3468pii
S0014-5793(11)00703-4journal_volume
585pub_type
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