Conservation of interphase chromatin nonhistone antigens as components of metaphase chromosomes.

abstract：:Cytosine in nucleic acids can be converted into N4-aminocytosine by treatment with a mixture of hydrazine and bisulfite. The hydrazino group thus formed at position 4 of the pyrimidine ring can be linked to a sulhydryl group in proteins by the use of bromopyruvate as a linker. Successful use of this scheme of chemical...

journal_title：FEBS letters

authors： Nitta N,Kuge O,Yui S,Tsugawa A,Negishi K,Hayatsu H

更新日期：1984-01-23 00:00:00

abstract：:Several clinical studies have shown that cyclosporin A (CsA) is effective for treating a variety of chronic inflammatory and autoimmune diseases. Because reactive oxygen species are believed to play a key role in the development of these diseases, causing cell apoptosis, we investigated whether CsA inhibits H2O2-induc...

journal_title：FEBS letters

authors： Sugano N,Ito K,Murai S

更新日期：1999-03-26 00:00:00

abstract：:A 569 bp probe against the beta-chain of hepatotropin was used to examine expression of RNA for this growth factor in human adult and foetal liver, foetal kidney and pancreas, and rat liver after partial hepatectomy. Low level expression of a 6 kb RNA occurred in human adult and normal rat liver. 70% hepatectomy incre...

journal_title：FEBS letters

authors： Selden C,Jones M,Wade D,Hodgson H

更新日期：1990-09-17 00:00:00

abstract：:DNA polymerase alpha-primase is a heterotetrameric complex essential for simian vacuolating virus 40 (SV40) DNA replication. We show that the C-terminal 67 amino acid residues of the human p180 subunit are essential for SV40 DNA replication as they are required for binding of the p68 subunit and play a role in the int...

journal_title：FEBS letters

authors： Smith RW,Nasheuer HP

更新日期：2002-09-11 00:00:00

abstract：:Steady-state kinetic analysis was performed on the reaction between D-fructose and ferricyanide with the quinohemoprotein fructose dehydrogenase from Gluconobacter species. The D-fructose oxidation dependence on the ferricyanide concentration resulted in a series of parallel reciprocal plots, and the reaction was assu...

journal_title：FEBS letters

authors： Marcinkeviciene J,Johansson G

更新日期：1993-02-22 00:00:00

abstract：:Sperm beta-acrosin activity is inhibited by suramin, a polysulfonated naphthylurea compound with therapeutic potential as a combined antifertility agent and microbicide. A kinetic analysis of enzyme inhibition suggests that three and four molecules of suramin bind to one molecule of ram and boar beta-acrosins respecti...

journal_title：FEBS letters

authors： Hermans JM,Haines DS,James PS,Jones R

更新日期：2003-06-05 00:00:00

abstract：:Inflammation is highly regulated by various agents. Unexpectedly, we report here that the damage-associated molecular pattern S100A9 protein, a potent neutrophil activator and inducer of cytokine production in monocytes, is not a direct activator of cytokine production in human neutrophils. However, S100A9 primed IL-8...

journal_title：FEBS letters

authors： Simard JC,Noël C,Tessier PA,Girard D

更新日期：2014-06-13 00:00:00

abstract：:We employed cDNA representational difference analysis to identify new genes that are upregulated by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in a human hepatoblastoma cell line HepG2. We isolated several TCDD-responsive cDNAs. Sequence analysis revealed that one of them encodes E16/CD98LC/hLAT1, an integral membrane...

journal_title：FEBS letters

authors： Sarkar D,Kambe F,Hirata A,Iseki A,Ohmori S,Seo H

更新日期：1999-12-03 00:00:00

abstract：:The less polar fluorescent analogue of ATP, 2'(3')-trinitrophenyl-5'-triphosphate bound to rat liver fatty acid-binding protein with high affinity (Kd 6.3 x 10(-6) M) and 1:1 molar stoichiometry. This probe bound to the fatty acid binding site of the protein and was displaced by oleic acid and oleoyl CoA. High concent...

journal_title：FEBS letters

authors： Sheridan M,Wilton DC

更新日期：1992-12-21 00:00:00

abstract：:To determine the amino acid residues required for the signal-transducing activity of the human c-Ha-Ras protein, we introduced point mutations at residues 45-54 near the 'effector region' (residues 32-40). We transfected PC12 cells with these mutant genes and also micro-injected the mutant proteins, bound with an unhy...

journal_title：FEBS letters

authors： Fujita-Yoshigaki J,Shirouzu M,Koide H,Nishimura S,Yokoyama S

更新日期：1991-12-09 00:00:00

abstract：:The nervous and immune systems have similar functional characteristics. Both have an intricate network of synaptic connections and an exquisite communication system that enable intercellular signal transduction. Although semaphorins were originally identified as guidance cues in neural development, accumulating eviden...

journal_title：FEBS letters

authors： Okuno T,Nakatsuji Y,Kumanogoh A

更新日期：2011-12-01 00:00:00

abstract：:A site-selective cAMP analog, 8-chloroadenosine 3',5'-cyclic monophosphate (8-Cl-cAMP), was demonstrated to be a potent inhibitor of both the monolayer and soft agar growth of normal rat kidney (NRK) fibroblasts that had been transformed with the v-Ki-ras oncogene or treated with transforming growth factor alpha (TGF ...

journal_title：FEBS letters

authors： Tortora G,Ciardiello F,Ally S,Clair T,Salomon DS,Cho-Chung YS

更新日期：1989-01-02 00:00:00

abstract：:In the present work, a new endopin-like serpin designed mEndopin 1B was purified from bovine muscle. Biochemical characterizations (amino acid sequencing and Maldi-Tof mass spectrometry peptide mapping) demonstrated that the purified protein is different from the previously described Endopin 1, renamed mEndopin 1A. Th...

journal_title：FEBS letters

authors： Herrera-Mendez CH,Brémaud L,Coulis G,Pélissier P,Sentandreu MA,Aubry L,Delourme D,Chambon C,Maftah A,Leveziel H,Ouali A

更新日期：2006-06-12 00:00:00

abstract：:The glucagon-like peptide 1 (7-37)/(7-36) amide (GLP-1) receptor belongs to a new subclass of seven transmembrane domain, G-protein coupled receptors comprising several receptors for peptide hormones. The receptors of this family share many common motifs including a relatively large N-terminal extracellular domain. Th...

journal_title：FEBS letters

authors： Wilmen A,Göke B,Göke R

更新日期：1996-11-25 00:00:00

abstract：:A novel type IV collagen-degrading metalloproteinase was purified from the conditioned media of a murine metastatic sarcoma cell line. The molecular weight of the purified enzyme was determined to be 100 kDa by SDS-PAGE, while 700 kDa by gel filtration suggesting that the enzyme has a multimer structure. This enzyme d...

journal_title：FEBS letters

authors： Kato Y,Ogawa K,Yamamoto S,Abe S,Kishi J,Hayakawa T

更新日期：1990-07-30 00:00:00

abstract：:Oligomeric structures of the four LOV domains in Arabidopsis phototropin1 (phot1) and 2 (phot2) were studied using crosslinking. Both LOV1 domains of phot1 and phot2 form a dimer independently on the light conditions, suggesting that the LOV1 domain can be a stable dimerization site of phot in vivo. In contrast, phot1...

journal_title：FEBS letters

authors： Katsura H,Zikihara K,Okajima K,Yoshihara S,Tokutomi S

更新日期：2009-02-04 00:00:00

abstract：:We report the identification, gene expression and biological activity of two parathyroid hormones (PTH; PTHA and PTHB), two PTH-related peptides (PTHrP; PTHrPA and PTHrPB) and a PTH-like ligand (PTH-L) with hybrid characteristics in puffer fishes (Takifugu rubripes and Tetraodon fluviatilis). Experimental data are con...

journal_title：FEBS letters

authors： Canario AV,Rotllant J,Fuentes J,Guerreiro PM,Rita Teodósio H,Power DM,Clark MS

更新日期：2006-01-09 00:00:00

abstract：:Tumor necrosis factor (TNF) has been shown to inhibit the growth of some cell types and stimulate the proliferation of others by a mechanism that is not understood. In the present study, we investigated the effect of transfection of NIH-3T3 cells with either the basic fibroblast growth factor gene (bFGF) or the kaposi...

journal_title：FEBS letters

authors： Aggarwal BB,Pocsik E,Totpal K

更新日期：1995-09-18 00:00:00

abstract：:Recently, we cloned several fluorescent proteins of different colors homologous to Aequorea victoria green fluorescent protein, which have great biotechnological potential as in vivo markers of gene expression. However, later investigations revealed severe drawbacks in the use of novel fluorescent proteins (FPs), in p...

journal_title：FEBS letters

authors： Yanushevich YG,Staroverov DB,Savitsky AP,Fradkov AF,Gurskaya NG,Bulina ME,Lukyanov KA,Lukyanov SA

更新日期：2002-01-30 00:00:00

abstract：:3C-like (3CL) protease is essential for the life cycle of severe acute respiratory syndrome-coronavirus (SARS-CoV) and therefore represents a key anti-viral target. A compound library consisting of 960 commercially available drugs and biologically active substances was screened for inhibition of SARS-CoV 3CL protease....

journal_title：FEBS letters

authors： Hsu JT,Kuo CJ,Hsieh HP,Wang YC,Huang KK,Lin CP,Huang PF,Chen X,Liang PH

更新日期：2004-09-10 00:00:00

abstract：:We have investigated whether the expression in Arabidopsis thaliana seeds of a transgene (the Phaseolus vulgaris arcelin (arc)5-I gene) could be enhanced by the simultaneous introduction of an antisense gene for an endogenous seed storage protein (2S albumin). Seeds of plants transformed with both the arc5-I gene and ...

journal_title：FEBS letters

authors： Goossens A,Van Montagu M,Angenon G

更新日期：1999-07-30 00:00:00

abstract：:Stable CHO cell clones which selectively express all three rat tachykinin receptors were established by transfection. The binding of radiolabled substance P and neurokinin A (substance K) to CHO clones expressing the NK1 and NK2 receptors, respectively, were saturatable and of high affinity (Kd = 0.17 nM (NK1); 3.4 nM...

journal_title：FEBS letters

authors： Gether U,Marray T,Schwartz TW,Johansen TE

更新日期：1992-01-27 00:00:00

abstract：:The human pathogen Vibrio vulnificus undergoes phase variation among colonial morphotypes, including a virulent opaque form which produces capsular polysaccharide (CPS) and a translucent phenotype that produces little or no CPS and is attenuated. Here, we found that a V. vulnificus mutant defective for RfaH antitermin...

journal_title：FEBS letters

authors： Garrett SB,Garrison-Schilling KL,Cooke JT,Pettis GS

更新日期：2016-12-01 00:00:00

abstract：:The bipartite human interleukin-4 (IL-4) receptor was functionally expressed in murine pro-B cells and activated by human IL-4 to evoke intracellular signaling. Mutual association of signal transducing proteins within the receptor complex was then studied in dependence of ligand stimulation. Besides ligand-induced rec...

journal_title：FEBS letters

authors： Erhardt I,Lischke A,Sebald W,Friedrich K

更新日期：1998-11-13 00:00:00

abstract：:Evidence has previously suggested that cellobiose:quinone oxidoreductase (CBQ) in cellulolytic cultures of Phanerochaete chrysosporium might be produced from cellobiose oxidase (CBO) by proteolytic cleavage. This study demonstrates that the ratio of CBO activity to (CBO + CBQ) activity declines with decreasing culture...

journal_title：FEBS letters

authors： Habu N,Samejima M,Dean JF,Eriksson KE

更新日期：1993-07-26 00:00:00

abstract：:Methyl-beta-cyclodextrin (MbetaCD) was used to explore a role for cholesterol-enriched plasma membrane microdomains in coupling lysophosphatidic acid (LPA) stimulation to phosphoinositide 3-kinase (PI3K) activation. Cholesterol depletion strongly inhibited the production of phosphatidylinositol 3,4-bisphosphate and ph...

journal_title：FEBS letters

authors： Peres C,Yart A,Perret B,Salles JP,Raynal P

更新日期：2003-01-16 00:00:00

abstract：:The rise of antibiotic resistance combined with the lack of new products entering the market has led to bacterial infections becoming one of the biggest threats to global health. Therefore, there is an urgent need to identify novel antibiotic targets, such as dihydrodipicolinate synthase (DHDPS), an enzyme involved in...

journal_title：FEBS letters

authors： Impey RE,Lee M,Hawkins DA,Sutton JM,Panjikar S,Perugini MA,Soares da Costa TP

更新日期：2020-05-01 00:00:00

abstract：:In Saccharomyces cerevisiae, four proteins, Shu1, Shu2, Psy3 and Csm2, form a stable SHU-complex both in vivo and in vitro. These proteins are involved in the early stages of the homologous recombination DNA damage repair process. In this paper, the crystal structure of the Psy3-Csm2 sub-complex is presented at 1.8Å r...

journal_title：FEBS letters

authors： She Z,Gao ZQ,Liu Y,Wang WJ,Liu GF,Shtykova EV,Xu JH,Dong YH

更新日期：2012-07-30 00:00:00

abstract：:Damage to the central nervous system triggers rapid activation and specific migration of glial cells towards the lesion site. There, glial cells contribute heavily to secondary neuronal changes that take place after lesion. In an attempt to identify the molecular cues of glial activation following brain trauma we perf...

journal_title：FEBS letters

authors： Bräuer AU,Nitsch R,Savaskan NE

更新日期：2004-04-09 00:00:00

abstract：:Lipopolysaccharide, the invariant structural component of Gram-negative bacteria, when present in minute amounts in the circulation in humans elicits 'endotoxic shock' syndrome, which is fatal in 60% of the cases. Polymyxin B (PMB), a cyclic cationic peptide, neutralizes the endotoxin, but also induces many harmful si...

journal_title：FEBS letters

authors： Thomas CJ,Surolia A

更新日期：1999-02-26 00:00:00