Abstract:
:Recently, we cloned several fluorescent proteins of different colors homologous to Aequorea victoria green fluorescent protein, which have great biotechnological potential as in vivo markers of gene expression. However, later investigations revealed severe drawbacks in the use of novel fluorescent proteins (FPs), in particular, the formation of tetramers (tetramerization) and high molecular weight aggregates (aggregation). In this report, we employ a mutagenic approach to resolve the problem of aggregation. The elimination of basic residues located near the N-termini of FPs results in the generation of non-aggregating versions of several FPs, specifically, drFP583 (DsRed), DsRed-Timer, ds/drFP616, zFP506, zFP538, amFP486, and asFP595.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Yanushevich YG,Staroverov DB,Savitsky AP,Fradkov AF,Gurskaya NG,Bulina ME,Lukyanov KA,Lukyanov SAdoi
10.1016/s0014-5793(01)03263-xkeywords:
subject
Has Abstractpub_date
2002-01-30 00:00:00pages
11-4issue
1-3eissn
0014-5793issn
1873-3468pii
S001457930103263Xjournal_volume
511pub_type
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