Abstract:
:Oligomeric structures of the four LOV domains in Arabidopsis phototropin1 (phot1) and 2 (phot2) were studied using crosslinking. Both LOV1 domains of phot1 and phot2 form a dimer independently on the light conditions, suggesting that the LOV1 domain can be a stable dimerization site of phot in vivo. In contrast, phot1-LOV2 is in a monomer-dimer equilibrium and phot2-LOV2 exists as a monomer in the dark. Blue light-induced a slight increase in the monomer population in phot1-LOV2, suggesting a possible blue light-inducible dissociation of dimers. Furthermore, blue light caused a band shift of the phot2-LOV2 monomer. CD spectra revealed the unfolding of helices and the formation of strand structures. Both light-induced changes were reversible in the dark.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Katsura H,Zikihara K,Okajima K,Yoshihara S,Tokutomi Sdoi
10.1016/j.febslet.2009.01.019subject
Has Abstractpub_date
2009-02-04 00:00:00pages
526-30issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(09)00029-5journal_volume
583pub_type
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