Identification of putative calcium channels in skeletal muscle microsomes.

abstract：:The fluorescence dye 1-anilinonaphthalene-8-sulfonate (ANS) was used as a probe of non-polar binding sites in 4-aminobutyrate aminotransferase. ANS binds to a single binding site of the dimeric protein with a Kd of 6 microM. Nanosecond emission anisotropy measurements were performed on the ANS-enzyme in an effort to d...

journal_title：FEBS letters

authors： Churchich JE,Kim DS,Schnackerz KD

更新日期：1983-08-22 00:00:00

abstract：:The sarcoplasmic reticulum Ca(2+)-ATPase loses hydrolytic activity and the ability to be phosphorylated by Pi following incubation with EDC [1-ethyl-3-(3-dimethylaminopropyl)carbodiimide]. 4 nmol of tempamine per mg SR protein can be coupled to either a glu or an asp side chain through the EDC reaction. Mg2+ protects ...

journal_title：FEBS letters

authors： Coan C,Jakobs P,Ji JY,Murphy AJ

更新日期：1993-11-29 00:00:00

abstract：:A metallothionein cDNA was generated from pea (Pisum sativum L.) roots, amplified by PCR and inserted into a plasmid for expression in E. coli. Purification of the resultant product generated 3 pools of cadmium-containing material after DEAE-cellulose chromatography. The amino acid composition of each was in excellent...

journal_title：FEBS letters

authors： Kille P,Winge DR,Harwood JL,Kay J

更新日期：1991-12-16 00:00:00

abstract：:HIV gp120 is specifically cleaved at a single site in the V3 loop between Arg315 and Ala316 by thrombin. Previous observations by others have indicated that binding to CD4 enhances the rate of V3 loop cleavage, and that this cleavage is a prerequisite for HIV infection. Other observations also suggest that the cleavag...

journal_title：FEBS letters

authors： Johnson ME,Lin Z,Padmanabhan K,Tulinsky A,Kahn M

更新日期：1994-01-03 00:00:00

abstract：:D-Aspartate increases human chorionic gonadotropin-induced testosterone production in purified rat Leydig cells. L-Aspartate, D-,L-glutamate or D-,L-asparagine could not substitute for D-aspartate and this effect was independent of glutamate receptor activation. Testosterone production was enhanced only in cells cultu...

journal_title：FEBS letters

authors： Nagata Y,Homma H,Lee JA,Imai K

更新日期：1999-02-12 00:00:00

abstract：:Treatment of human lymphoblastoid (Daudi) cells with interferons inhibits cell proliferation in culture within 24 h. The failure of cell growth has been shown to be associated with impaired processing and decreased stability of newly replicated DNA. Because there is a close relationship between DNA replication and pro...

journal_title：FEBS letters

authors： Clemens MJ,McNurlan MA,Moore G,Tilleray VJ

更新日期：1984-06-04 00:00:00

abstract：:Sequence comparisons among methionyl-tRNA synthetases from different organisms reveal only one block of homology beyond the last beta strand of the mononucleotide fold. We have introduced a series of semi-conservative amino acid replacements in the conserved motif of yeast methionyl-tRNA synthetase. The results indica...

journal_title：FEBS letters

authors： Despons L,Walter P,Senger B,Ebel JP,Fasiolo F

更新日期：1991-09-09 00:00:00

abstract：:The [Cu(I)-Cu(II)] half-met form of the dinuclear copper site of tyrosinase has been probed by continuous wave electron paramagnetic resonance (EPR) and hyperfine sublevel correlation (HYSCORE) spectroscopy in the presence and absence of inhibitors. In all cases the EPR spectrum is indicative of a d(x(2)-y(2)) ground ...

journal_title：FEBS letters

authors： van Gastel M,Bubacco L,Groenen EJ,Vijgenboom E,Canters GW

更新日期：2000-06-02 00:00:00

abstract：:We characterized the promoter activity of a medaka fish intestinal guanylyl cyclase gene, OlGC6, by assay of enzyme activity in response to various promoter-luciferase fusion gene constructs introduced into CACO-2 cells and medaka fish embryos. A transient transfection assay of the various fusion gene constructs showe...

journal_title：FEBS letters

authors： Nakauchi M,Suzuki N

更新日期：2003-02-11 00:00:00

abstract：:Two fatty acid ethyl ester (FAEE) synthase isoenzymes purified from human myocardium were reported to be glutathione S-transferases (GST) [(1989) Proc. Natl. Acad. Sci. USA 86, 4470-4473; and (1989) J. Clin. Invest. 84, 1942-1946]. In the present study, the FAEE synthase activity of several purified and well character...

journal_title：FEBS letters

authors： Suzuki T,Kovacs MS,Board PG

更新日期：1990-11-26 00:00:00

abstract：:The fidelity of tRNA aminoacylation is dependent in part on amino acid editing mechanisms. A hydrolytic activity that clears mischarged tRNAs typically resides in an active site on the tRNA synthetase that is distinct from its synthetic aminoacylation active site. A second pre-transfer editing pathway that hydrolyzes ...

journal_title：FEBS letters

authors： Martinis SA,Boniecki MT

更新日期：2010-01-21 00:00:00

abstract：:Gangliosides and neutral glycolipids of muscles from normal and dystrophic chickens were studied. Total glycolipid content of the degenerating muscles was higher than the normal muscles. In addition, the myopathic muscles contained a ganglioside which was absent in the unaffected muscles from normal and dystrophic chi...

journal_title：FEBS letters

authors： Kundu SK,Misra LK,Luthra MG

更新日期：1982-12-27 00:00:00

abstract：:Previous data have suggested an involvement of MDR/PGP-like ABC transporters in transport of the plant hormone auxin and, recently, AtPGP1 has been demonstrated to catalyze the primary active export of auxin. Here we show that related isoform AtPGP4 is expressed predominantly during early root development. AtPGP4 loss...

journal_title：FEBS letters

authors： Santelia D,Vincenzetti V,Azzarello E,Bovet L,Fukao Y,Düchtig P,Mancuso S,Martinoia E,Geisler M

更新日期：2005-10-10 00:00:00

abstract：:A cytochrome c (cyt. c) was solubilized with Triton-X-100 and co-purified with cytochrome c oxidase from membranes of chemotrophically grown cells of Rhodopseudomonas capsulata. Cyt. c and cytochrome oxidase were separated on Sephadex G-50 columns. Antibodies against cytochrome c2 from the same bacterium did not cross...

journal_title：FEBS letters

authors： Hüdig H,Drews G

更新日期：1983-02-21 00:00:00

abstract：:The phosphatidylinositol 3-kinase/Akt pathway is responsible for key aspects of tumor progression, and is frequently hyperactivated in cancer. We have recently identified palladin, an actin-bundling protein that functions to control the actin cytoskeleton, as an Akt1-specific substrate that inhibits breast cancer cell...

journal_title：FEBS letters

authors： Chin YR,Toker A

更新日期：2010-12-01 00:00:00

abstract：:The effect of all possible codons corresponding to the second and third amino acid (isoleucine and threonine) on the expression level of hirudin in E. coli has been analysed. These levels could not be correlated with changes in primary and secondary mRNA structure. A decrease in the rate of synthesis and of product ac...

journal_title：FEBS letters

authors： Degryse E

更新日期：1990-08-20 00:00:00

abstract：:Detailed structural models of di-cluster seven-iron ferredoxins constitute a valuable resource for folding and stability studies relating the metal cofactors' role in protein stability. The here reported, hemihedric twinned crystal structure at 2.0 A resolution from Acidianus ambivalens ferredoxin, shows an integral 1...

journal_title：FEBS letters

authors： Frazão C,Aragão D,Coelho R,Leal SS,Gomes CM,Teixeira M,Carrondo MA

更新日期：2008-03-05 00:00:00

abstract：:We have recorded (13)C nuclear magnetic resonance (NMR) spectra of [3-(13)C]Ala, [1-(13)C]Val-labeled pharaonis phoborhodopsin (ppR or sensory rhodopsin II) incorporated into egg PC (phosphatidylcholine) bilayer, by means of site-directed high-resolution solid-state NMR techniques. Seven (13)C NMR signals from transme...

journal_title：FEBS letters

authors： Arakawa T,Shimono K,Yamaguchi S,Tuzi S,Sudo Y,Kamo N,Saitô H

更新日期：2003-02-11 00:00:00

abstract：:H2O2 addition to the oxidized cytochrome c oxidase reconstituted in liposomes brings about a red shift of the Soret band of the enzyme and an increased absorption in the visible region with two distinct peaks at approximately 570 and 605 nm. Throughout pH range 6-8.5, the spectral changes at 570 nm and in the Soret ba...

journal_title：FEBS letters

authors： Vygodina T,Konstantinov AA

更新日期：1987-07-27 00:00:00

abstract：:Transient-receptor-potential channels (TRPs) underlie the sensing of chemicals, heat, and mechanical force. We expressed the rat TRPV1 and TRPV4 subtypes in yeast and monitored their activities in vivo as Ca(2+) rise using transgenic aequorin. Heat and capsaicin activate TRPV1 but not TRPV4 in yeast. Hypotonic shocks ...

journal_title：FEBS letters

authors： Loukin SH,Su Z,Kung C

更新日期：2009-02-18 00:00:00

abstract：:Comparison of the phosphotyrosine-specific dephosphorylation of the autophosphorylated receptors for epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) in Swiss 3T3 cell membranes by the endogenous phosphatases revealed striking differences. EGF receptor dephosphorylation was clearly faster than P...

journal_title：FEBS letters

authors： Böhmer FD,Böhmer SA,Heldin CH

更新日期：1993-10-04 00:00:00

abstract：:The function of the amino- and carboxyl-terminal domains of the yeast plasma membrane H+-ATPase have been investigated by constructing deletions in vitro and selectively expressing the mutant enzymes in vivo. The first 27 amino acids are dispensable but deletion of a further 33 amino acids greatly decreases the appear...

journal_title：FEBS letters

authors： Portillo F,de Larrinoa IF,Serrano R

更新日期：1989-04-24 00:00:00

abstract：:DNA polymerase (pol) λ, one of the 15 cellular pols, belongs to the X family. It is a small 575 amino-acid protein containing a polymerase, a dRP-lyase, a proline/serine rich and a BRCT domain. Pol λ shows various enzymatic activities including DNA polymerization, terminal transferase and dRP-lyase. It has been implic...

journal_title：FEBS letters

authors： Markkanen E,van Loon B,Ferrari E,Hübscher U

更新日期：2011-09-16 00:00:00

abstract：:Adrenomedullin (ADM) functions as a survival factor against hypoxic cell death. However, molecular mechanisms underlying the cell survival pathway remain largely unknown. In this report, we showed that ADM suppressed reactive oxygen species (ROS) increase by inhibiting reduction of glutathione (GSH) level in hypoxia/r...

journal_title：FEBS letters

authors： Kim SM,Kim JY,Lee S,Park JH

更新日期：2010-01-04 00:00:00

abstract：:Published amino acid sequences for nitrogenase component polypeptides were compared with those of other proteins which also bind adenine nucleotides. Three sequences which might contribute to an adenine nucleotide-binding domain were found for the Fe-protein component of nitrogenase. The beta-subunit of the MoFe-prote...

journal_title：FEBS letters

authors： Robson RL

更新日期：1984-08-06 00:00:00

abstract：:Mitochondrial fatty acid synthesis is catalyzed by a dissociated fatty acid synthase similar to those of plant plastids and bacteria. The crystal structure of a mitochondrial beta-ketoacyl-[acyl carrier protein] synthase (mtKAS), namely that from Arabidopsis thaliana, has been determined for the first time. This enzym...

journal_title：FEBS letters

authors： Olsen JG,Rasmussen AV,von Wettstein-Knowles P,Henriksen A

更新日期：2004-11-05 00:00:00

abstract：:Two RNA silencing-related phenomena, quelling and meiotic silencing by unpaired DNA (MSUD) have been identified in the fungus Neurospora crassa. Similar to the case with the siRNA and miRNA pathways in Drosophila, different sets of protein components including RNA-dependent RNA polymerase, argonaute and dicer, are use...

journal_title：FEBS letters

authors： Nakayashiki H

更新日期：2005-10-31 00:00:00

abstract：:Five-lipoxygenase-activating protein (FLAP) is usually described as an essential protein to activate the leukotriene (LTs) synthesis via the 5-lipoxygenase pathway. In the enterocyte model HT29 cl.19A cell line, 5-lipoxygenase metabolism was found despite the lack of FLAP expression. Therefore HT29 cl.19A represents a...

journal_title：FEBS letters

authors： Battu S,Beneytout JL,Pairet M,Rigaud M

更新日期：1998-10-16 00:00:00

abstract：:The sarcoplasmic reticulum-bound creatine kinase from rabbit skeletal muscle was inhibited by the nitric oxide donor S-nitrosoglutathione (GSNO). This led to a decrease in Ca2+ uptake in sarcoplasmic reticulum vesicles when the transport was driven by ATP generated from phosphocreatine and ADP. In contrast, the Ca 2+ ...

journal_title：FEBS letters

authors： Wolosker H,Panizzutti R,Engelender S

更新日期：1996-09-02 00:00:00

abstract：:Actin filaments copolymerized with both intact and chemically modified actin monomers restored their sliding activity when they were supplemented with tropomyosin extracted from skeletal muscle. In contrast, the ATPase activation of the copolymers was decreased when supplemented with tropomyosin. The results indicate ...

journal_title：FEBS letters

authors： Honda H,Kitano Y,Hatori K,Matsuno K

更新日期：1996-03-25 00:00:00