The binding of the fluorescent ATP analogue 2'(3')-trinitrophenyladenosine-5'-triphosphate to rat liver fatty acid-binding protein.

abstract：:To assess the role of crystallinity in biological response, we quantitated the generation of reactive oxygen metabolites in human polymorphonuclear leukocytes (PMN) with a chemiluminescence assay using three hydroxyapatite preparations with sintering temperatures of 1,200 degrees C and 900 degrees C and a drying tempe...

journal_title：FEBS letters

authors： Nagase M,Nishiya H,Abe Y

更新日期：1993-07-05 00:00:00

abstract：:Using antibody against the Rho binding domain of ROKalpha, two neuronal phosphoproteins of 62 and 80 kDa were co-immunoprecipitated from brain extracts. Peptide analysis revealed their identity as collapsin response mediator proteins (CRMPs); p62 was CRMP-2 whereas p80 was a novel splice form of CRMP-1 with an extende...

journal_title：FEBS letters

authors： Leung T,Ng Y,Cheong A,Ng CH,Tan I,Hall C,Lim L

更新日期：2002-12-18 00:00:00

abstract：:In unicellular species codon usage is determined by mutational biases and natural selection. Among prokaryotes, the influence of these factors is different if the genome is skewed towards AT or GC, since in AT-rich organisms translational selection is absent. On the other hand, in AT-rich unicellular eukaryotes the tw...

journal_title：FEBS letters

authors： Naya H,Romero H,Carels N,Zavala A,Musto H

更新日期：2001-07-20 00:00:00

abstract：:miR398 links expression of the three major chloroplast copper proteins, plastocyanin, CCS1 and Csd2, to copper availability. miR398 abundance was stronger plastocyanin-controlled in accessions from cold and continental habitats (Kas-1, Ms-0, WS) than in Cvi-0 and Col-0. Target gene regulation was broken for Csd2 in Cv...

journal_title：FEBS letters

authors： Juszczak I,Baier M

更新日期：2012-09-21 00:00:00

abstract：:A lowered efficiency of oxidative phosphorylation was recently found in a Leber hereditary optic neuropathy (LHON) proband carrying a mutation in the mtDNA gene for subunit 6 of the membrane-bound F0 segment of the F1F0-ATP synthase [9]. This phenotype was transferred to cytoplasmic hybrid cells together with the muta...

journal_title：FEBS letters

authors： Majander A,Lamminen T,Juvonen V,Aula P,Nikoskelainen E,Savontaus ML,Wikström M

更新日期：1997-07-28 00:00:00

abstract：:LIM-homeodomain (LIM-HD) proteins are evolutionary conserved developmental transcription factors. LIM-HD Lmx1a and Lmx1b orchestrate complex temporal and spatial gene expression of the dopaminergic pathway, and evidence shows they are also involved in adult neuronal homeostasis. In this review, the multiple roles play...

journal_title：FEBS letters

authors： Doucet-Beaupré H,Ang SL,Lévesque M

更新日期：2015-12-21 00:00:00

abstract：:Upon in vitro translation of mRNAs from slow (soleus) muscles of the mouse a hitherto undescribed translation product has been detected that was absent from fast skeletal muscles and was termed p19/6.8 according to its position in 2-dimensional gels. mRNA for p19/6.8 was also found in the ventricle of the heart. p19/6...

journal_title：FEBS letters

authors： Kluxen FW

更新日期：1988-03-28 00:00:00

abstract：:Neurotrophins support neuronal survival, development, and plasticity through processes requiring gene expression. We studied how vgf target gene transcription is mediated by a critical promoter region containing E-box, CCAAT and cAMP response element (CRE) sites. The p300 acetylase was present in two distinct protein ...

journal_title：FEBS letters

authors： Mandolesi G,Gargano S,Pennuto M,Illi B,Molfetta R,Soucek L,Mosca L,Levi A,Jucker R,Nasi S

更新日期：2002-01-02 00:00:00

abstract：:Human hepatocellular carcinoma (HCC) is one of the most common tumors worldwide, in which the genetic mechanisms of oncogenesis are still unclear. To investigate whether the genomic DNA copy number alterations may contribute to primary HCC, the cDNA microarray-based comparative genomic hybridization (CGH) analysis was...

journal_title：FEBS letters

authors： Huang J,Sheng HH,Shen T,Hu YJ,Xiao HS,Zhang Q,Zhang QH,Han ZG

更新日期：2006-06-26 00:00:00

abstract：:The phylogenetic distribution of divergently related protein families into the three domains of life (archaea, bacteria and eukaryotes) can signify the presence or absence of entire cellular processes in these domains and their ancestors. We can thus study the emergence of the major transitions during cellular evoluti...

journal_title：FEBS letters

authors： Ouzounis C,Kyrpides N

更新日期：1996-07-22 00:00:00

abstract：:A synthetic form of human pancreatic growth hormone releasing factor (GRF-44-NH2) was shown to be a potent stimulator of growth hormone (GH) secretion and cellular cyclic AMP levels in cultured sheep pituitary cells. A small dose-dependent stimulation of prolactin secretion was also observed. Somatostatin (0.5 microM)...

journal_title：FEBS letters

authors： Law GJ,Ray KP,Wallis M

更新日期：1984-01-23 00:00:00

abstract：:Endothelial monocyte-activating polypeptide II (EMAP II) is a proinflammatory cytokine and a chemoattractant for leukocytes. The mature cytokine is formed in apoptotic cells by cleavage of the precursor proEMAP II. Here we show that caspase-7 is capable of cleaving proEMAP II in vitro. A proEMAP II mutant, in which th...

journal_title：FEBS letters

authors： Behrensdorf HA,van de Craen M,Knies UE,Vandenabeele P,Clauss M

更新日期：2000-01-21 00:00:00

abstract：:Botulinum neurotoxins (BoNTs), the causative agent of human botulism, are the most potent naturally occurring toxins known. BoNT/A1, the most studied BoNT, is also used as an important biopharmaceutical. In this study, the biological activity of BoNT/A1 is compared to that of BoNT/A2 using neuronal cell models. The da...

journal_title：FEBS letters

authors： Pier CL,Chen C,Tepp WH,Lin G,Janda KD,Barbieri JT,Pellett S,Johnson EA

更新日期：2011-01-03 00:00:00

abstract：:Under physiological conditions significant amounts of low-density lipoprotein LDL particles ar taken up by cells independently of specific high-affinity LDL receptors (apo-B receptors). Previously it was established that some cells contain surface sites capable of binding liposomes. We proposed that liposome-binding s...

journal_title：FEBS letters

authors： Galkina SI,Ivanov VV,Preobrazhensky SN,Margolis LB,Bergelson LD

更新日期：1991-08-05 00:00:00

abstract：:Human hepatocellular carcinoma cells (Hep G2) were shown to secrete apo A-I as a proprotein . No apo A-I synthesis could be detected with endothelial cells from human umbilical cord veins. Conversion of proapo A-I into apo A-I is a slow (of the order of hours) process, mediated by a Ca2+/Mg2+-dependent enzyme which is...

journal_title：FEBS letters

authors： Kooistra T,van Hinsbergh V,Havekes L,Kempen HJ

更新日期：1984-05-07 00:00:00

abstract：:Development of targeted therapies for triple-negative breast cancer (TNBC, a more aggressive subtype) is an unmet medical need. We analyzed data from 887 patients with invasive breast cancer and observed that increased Wnt and histone deacetylase (HDAC) activities are associated with estrogen receptor 1 (ESR1) and pro...

journal_title：FEBS letters

authors： Sulaiman A,Sulaiman B,Khouri L,McGarry S,Nessim C,Arnaout A,Li X,Addison C,Dimitroulakos J,Wang L

更新日期：2016-12-01 00:00:00

abstract：:An Arabidopsis cDNA coding for a previously uncharacterized isoform of inorganic pyrophosphatase was isolated. It was used to complement an E. coli mutant, demonstrating that it coded for an active enzyme. MgCl(2) was necessary for the protein's activity, whilst NaF inhibited it. The K(m) for pyrophosphate and the pH ...

journal_title：FEBS letters

authors： Schulze S,Mant A,Kossmann J,Lloyd JR

更新日期：2004-05-07 00:00:00

abstract：:Rho proteins are down-regulated in vivo by specific GTPase activating proteins (RhoGAP). We have functionally studied three Saccharomyces cerevisiae putative RhoGAP. By first identifying Rho partners with a systematic two-hybrid approach and then using an in vitro assay, we have demonstrated that the Bag7 protein stim...

journal_title：FEBS letters

authors： Roumanie O,Weinachter C,Larrieu I,Crouzet M,Doignon F

更新日期：2001-10-05 00:00:00

abstract：:The substrate peptide bond cleaved by cathepsins B and L is determined not by the amino acid contributing the carboxyl group to this bond as in the case of serine proteases but rather by the presence of a neighboring amino acid with a large hydrophobic side chain. From a study of the inhibitory potency in a series, Cb...

journal_title：FEBS letters

authors： Kirschke H,Wikstrom P,Shaw E

更新日期：1988-02-08 00:00:00

abstract：:We previously showed that autocrine tumor necrosis factor (TNF) production in the TNF-sensitive L929sA fibrosarcoma cell line induced TNF resistance, which is correlated with downmodulation of both TNF receptors on the cell surface. We now analyzed whether autocrine TNF production also interfered with intracellular TN...

journal_title：FEBS letters

authors： Decoster E,Vandevoorde V,Beyaert R,Haegeman G,Fiers W

更新日期：1997-10-20 00:00:00

abstract：:Using the method of contextual analysis, we have studied the collection of somatic mutations in immunoglobulin genes. It has been found that the emergence of somatic mutations can be based on the reparation of complementarity violations in the heteroduplexes corresponding to complementary palindromes or direct repeats...

journal_title：FEBS letters

authors： Kolchanov NA,Solovyov VV,Rogozin IB

更新日期：1987-04-06 00:00:00

abstract：:Plants have evolved to cope with fluctuations in water supply by gating their water channels known as aquaporins. During flooding, a rapid drop of cytosolic pH due to anoxia leads to a simultaneous closure of the aquaporins in the plasma membrane. The closing mechanism has been suggested to involve a conserved histidi...

journal_title：FEBS letters

authors： Frick A,Järvå M,Törnroth-Horsefield S

更新日期：2013-04-02 00:00:00

abstract：:To investigate the cis-acting DNA elements that are involved in the regulation of class I major histocompatibility complex genes by interferon, several promoter fragments of the H-2Kk gene were linked to the reporter chloramphenicol acetyl transferase (CAT) gene, and the CAT expression was analyzed in stable transfect...

journal_title：FEBS letters

authors： Pascucci A,Pannuti A,La Mantia G,Lania L

更新日期：1988-01-04 00:00:00

abstract：:Contactin and TAG-1 are glycan phosphatidyl inositol (GPI)-anchored cell adhesion molecules that play a crucial role in the organization of axonal subdomains at the node of Ranvier of myelinating fibers. Contactin and TAG-1 mediate axo-glial selective interactions in association with Caspr-family molecules at paranode...

journal_title：FEBS letters

authors： Labasque M,Faivre-Sarrailh C

更新日期：2010-05-03 00:00:00

abstract：:(7-methoxycoumarin-4-yl)Acetyl-Pro-Leu-Gly-Leu-(3-[2,4-dinitrophenyl]-L- 2,3-diaminopropionyl)-Ala-Arg-NH2 (Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2) has been synthesised as a fluorogenic substrate for the matrix metalloproteinases. The highly fluorescent 7-methoxycoumarin group is efficiently quenched by energy transfer t...

journal_title：FEBS letters

authors： Knight CG,Willenbrock F,Murphy G

更新日期：1992-01-27 00:00:00

abstract：:A recent report of electrical measurements on oriented bacteriorhodopsin in gels [(1986) FEBS Lett. 195, 164 168] concluded that low concentrations of diamines reversed the direction of the proton pump. Calculations are presented which show that in low diamine concentrations, charge displacements of the counterion atm...

journal_title：FEBS letters

authors： Marinetti T

更新日期：1987-05-25 00:00:00

abstract：:Pyruvate dehydrogenase (E1p) is one of the components of the pyruvate dehydrogenase multienzyme complex (PDHC). Previously, it was shown that the N-terminal domain of E1p is involved in its binding to the core component (E2p) of PDHC. We constructed point mutations in this domain (D17Q, D17R, E20Q, E20R, D24Q and D24R...

journal_title：FEBS letters

authors： Hengeveld AF,de Kok A

更新日期：2002-07-03 00:00:00

abstract：:In washed anterior pituitary membranes, there is enough GTP to occupy Ns and therefore to obtain activation of adenylate cyclase by vasointestinal peptide. GTP concentrations needed to obtain adenylate cyclase inhibition by dopamine (above 5 X 10- M) stimulate the adenylate cyclase. The dopamine effect is a blockade o...

journal_title：FEBS letters

authors： Bockaert J,Sebben-Perez M

更新日期：1983-09-05 00:00:00

abstract：:As yet, the physiological significance of hydroxylation of anandamide and linoleoyl amides is unknown. Therefore, we investigated whether hydroxylation of ODNHEtOH and ODNH2 influences their binding abilities to the CB-1 receptor and whether it alters their reactivity towards a fatty acid amide hydrolase (FAAH) from r...

journal_title：FEBS letters

authors： van der Stelt M,Paoletti AM,Maccarrone M,Nieuwenhuizen WF,Bagetta G,Veldink GA,Finazzi Agrò A,Vliegenthart JF

更新日期：1997-10-06 00:00:00

abstract：:Phosphorylation of the 20 kDa myosin light chain from smooth muscle by five different kinases was investigated. Three of the kinases (myosin light chain kinase, phosphorylase kinase, and cAMP-dependent protein kinase) phosphorylate serine residues, the fourth (casein-kinase-2) mainly threonine, and the fifth (glycogen...

journal_title：FEBS letters

authors： Singh TJ,Akatsuka A,Huang KP

更新日期：1983-08-08 00:00:00