Abstract:
:An Arabidopsis cDNA coding for a previously uncharacterized isoform of inorganic pyrophosphatase was isolated. It was used to complement an E. coli mutant, demonstrating that it coded for an active enzyme. MgCl(2) was necessary for the protein's activity, whilst NaF inhibited it. The K(m) for pyrophosphate and the pH optimum of the protein was determined. The gene coding for this protein was expressed in all tissues, and its expression in rosette leaves was induced by incubation on metabolizable sugars. In vitro import experiments demonstrated that the protein could be imported into chloroplasts and localized to the stromal compartment.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Schulze S,Mant A,Kossmann J,Lloyd JRdoi
10.1016/j.febslet.2004.03.080keywords:
subject
Has Abstractpub_date
2004-05-07 00:00:00pages
101-5issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579304003898journal_volume
565pub_type
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