Abstract:
:Currently a central question that remains unresolved is the potential importance of phospholipase C (PLC)-gamma1 in cell mitogenesis. In this study, we introduced wild-type PLC-gamma1 plasmid and mutants Y771F and Y783F into CCL-39 fibroblasts and investigated their effect on host cell functions. To our surprise, Y771F and Y783F plasmids appeared to have opposite effects on CCL-39 cell actin-cytoskeleton organization and cell proliferation. Y771F transfectants increased cell proliferation by two-fold. Y783F transfectants showed much thicker actin filaments and decreased cell growth rate by 50%. These results suggest that PLC-gamma1 mutations have an essential impact on cell mitogenesis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Pei ZD,Williamson JRdoi
10.1016/s0014-5793(98)00063-5subject
Has Abstractpub_date
1998-02-13 00:00:00pages
53-6issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(98)00063-5journal_volume
423pub_type
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