Abstract:
:Mitochondrial fatty acid synthesis is catalyzed by a dissociated fatty acid synthase similar to those of plant plastids and bacteria. The crystal structure of a mitochondrial beta-ketoacyl-[acyl carrier protein] synthase (mtKAS), namely that from Arabidopsis thaliana, has been determined for the first time. This enzyme accomplishes the vital condensation steps in constructing fatty acid carbon skeletons. The product profile of mtKAS is unusual in that C8 and C(14-16) fatty acyl chains predominate. An enzyme architecture that likely is the basis for the observed bimodal profile of mtKAS products can be derived from the shape of the acyl binding pocket.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Olsen JG,Rasmussen AV,von Wettstein-Knowles P,Henriksen Adoi
10.1016/j.febslet.2004.10.007keywords:
subject
Has Abstractpub_date
2004-11-05 00:00:00pages
170-4issue
1-2eissn
0014-5793issn
1873-3468pii
S001457930401227Xjournal_volume
577pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Two RNA silencing-related phenomena, quelling and meiotic silencing by unpaired DNA (MSUD) have been identified in the fungus Neurospora crassa. Similar to the case with the siRNA and miRNA pathways in Drosophila, different sets of protein components including RNA-dependent RNA polymerase, argonaute and dicer, are use...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2005.08.016
更新日期:2005-10-31 00:00:00
abstract::Siamycin I disrupts growth and quorum sensing in Enterococcus faecalis. Using purified intact protein, we demonstrate here that quorum membrane sensor kinase FsrC is a direct target of siamycin I, reducing pheromone-stimulated autophosphorylation activity by up to 91%. Inhibition was non-competitive with ATP as substr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.07.026
更新日期:2011-09-02 00:00:00
abstract::Alterations in protein composition or dosage within chromatin may trigger changes in processes such as gene expression and DNA repair. Through transposon mutagenesis and targeted gene deletions in haploids and diploids of Saccharomyces cerevisiae, we identified mutations that affect telomeric silencing in genes encodi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.01.011
更新日期:2008-02-20 00:00:00
abstract::Vacuolar membrane H(+)-translocating pyrophosphatase (H(+)-PPase) was purified from pumpkin seedlings. Its enzymatic properties including molecular size of constituting polypeptide (75 kDa) were very similar to those of mung bean H(+)-PPase [(1989) J. Biol. Chem. 264, 20068-20073]. The native, functional molecular siz...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81254-6
更新日期:1991-09-23 00:00:00
abstract::T cells are stimulated by the engagement of antigen, cytokine, pathogen, and hormone receptors. While research performed over many years has focused on deciphering the molecular components of these pathways, recent data underscore the importance of the metabolic environment in conditioning responses to receptor engage...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12849
更新日期:2017-10-01 00:00:00
abstract::An efficient method is presented for the production of intact mammalian prion proteins and partial sequences thereof. As an illustration we describe the production of polypeptides comprising residues 23-231, 81-231, 90-231 and 121-231 of the human prion protein (hPrP). Polypeptides were expressed as histidine tail fus...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01330-6
更新日期:1997-11-17 00:00:00
abstract::14-3-3 proteins were the first signaling molecules to be identified as discrete phosphoserine/threonine binding modules. This family of proteins, which includes seven isotypes in human cells and up to 15 in plants, plays critical roles in cell signaling events that control progress through the cell cycle, transcriptio...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(01)03288-4
更新日期:2002-02-20 00:00:00
abstract::Anaerobic biosynthesis of carotenoids in the purple facultative photosynthetic bacterium Rhodobacter sphaeroides was studied using mass spectrometry. We have demonstrated that (18)O from H2(18)O was incorporated into the 1-methoxy group of spheroidene and spheroidenone, the two major carotenoids produced by this bacte...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00006-9
更新日期:1997-02-10 00:00:00
abstract::Chromogranin A is known to undergo pH induced conformational changes, and the difference in conformation is supposed to be responsible for the difference in Ca2+ binding property. To gain insight regarding the overall structure and the nature of pH-induced conformational changes of chromogranin A, limited trypsin dige...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80715-7
更新日期:1993-11-22 00:00:00
abstract::Several myeloid precursors of antibacterial peptides have recently been shown to share homologous pre- and pro-regions. Taking advantage of this homology, a novel cDNA was cloned from pig bone marrow RNA. This encodes a 166-residue polypeptide with highly conserved pre- (29 residues) and pro- (101 residues) sequences,...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80214-9
更新日期:1994-01-17 00:00:00
abstract::Diphtheria toxin fragment A is able to inhibit protein synthesis in the eukaryotic cell by ADP-ribosylating the diphthamide residue of elongation factor-2 (EF-2) [(1980) J. Biol. Chem. 255, 10710-10720]. The reaction requires NAD as ADP-ribose donor. This work reports on the capacity of an NAD analog, the nicotinamide...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80006-5
更新日期:1985-10-28 00:00:00
abstract::10Sa RNA is a bacterial small stable RNA, in which the 5'- and 3'-end sequences are folded into a tRNA-like structure. The RNA accepts alanine in vitro, and interacts with 70S ribosomes in the cells. In this study, we examined the ribosome-binding properties of Escherichia coli 10Sa RNA in vivo, and found that the ami...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01330-0
更新日期:1996-12-16 00:00:00
abstract::Casein phosphopeptides beta-CN(1-25)4P and alpha(s1)-CN(59-79)5P, from beta- and alpha(s1)-casein, respectively, both carrying the characteristic 'acidic motif' Ser(P)-Ser(P)-Ser(P)-Glu-Glu, were chemically synthesized and administered to HT-29 cells differentiated in culture, which are a used model of intestinal epit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00741-5
更新日期:2003-09-11 00:00:00
abstract::Zinc cations at concentrations of 0.2 mM and greater catalyzed specific phosphorylation, by ATP, of two membrane-associated proteins from rat hippocampus. These proteins, corresponding to molecular weights of 60 and 49 kDa, were phosphorylated primarily at tyrosine residues. The 60-kDa protein was identified as p60c-s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80534-n
更新日期:1992-06-01 00:00:00
abstract::Oligomeric structures of the four LOV domains in Arabidopsis phototropin1 (phot1) and 2 (phot2) were studied using crosslinking. Both LOV1 domains of phot1 and phot2 form a dimer independently on the light conditions, suggesting that the LOV1 domain can be a stable dimerization site of phot in vivo. In contrast, phot1...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.01.019
更新日期:2009-02-04 00:00:00
abstract::The Mahogany/Attractin gene (Atrn) has been proposed as a downstream mediator of Agouti signaling because yellow hair color and obesity in lethal yellow (A(y)) mice are suppressed by the mahogany (Atrn(mg)) mutation. The present study examined the distribution of Atrn mRNA in the brain and spinal cord by in situ hybri...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01494-5
更新日期:1999-11-26 00:00:00
abstract::Periplasmic adaptor proteins are essential components of bacterial tripartite multidrug efflux pumps. Here we report the 2.35 Å resolution crystal structure of the BesA adaptor from the spirochete Borrelia burgdorferi solved using selenomethionine derivatized protein. BesA shows the archetypal linear, flexible, multi-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.06.056
更新日期:2013-09-17 00:00:00
abstract::Many biochemical reactions are confined to interfaces, such as membranes or cell walls. Despite their importance, no canonical rate laws describing the kinetics of surface-active enzymes exist. Combining the approach chosen by Michaelis and Menten 100 years ago with concepts from surface chemical physics, we here pres...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.07.026
更新日期:2013-09-02 00:00:00
abstract::Using a variety of peptide analogues of oxytocin (OT) and Arg8-vasopressin (AVP), OT-mediated induction of urokinase-type plasminogen activator (uPA) was examined in LLC-PK1 renal epithelial cells, which possess distinct high-affinity receptors of both the OT- and vasopressin renal (V2-) types. OT or OT-receptor speci...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81149-t
更新日期:1993-01-04 00:00:00
abstract::A key step in sulfate assimilation into cysteine is the reduction of sulfite to sulfide by sulfite reductase (SiR). This enzyme is encoded by three genes in the moss Physcomitrella patens. To obtain a first insight into the roles of the individual isoforms, we deleted the gene encoding the SiR1 isoform in P. patens by...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.03.034
更新日期:2010-06-03 00:00:00
abstract::Interleukin-22 (IL-22) plays an important role in the regulation of immune and inflammatory responses in mammals. The IL-22 binding protein (IL-22BP), a soluble receptor that specifically binds IL-22, prevents the IL-22/interleukin-22 receptor 1 (IL-22R1)/interleukin-10 receptor 2 (IL-10R2) complex assembly and blocks...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.03.006
更新日期:2009-04-02 00:00:00
abstract::Thimet oligopeptidase (EC 3.4.24.15; TOP) is a Zn(II) endopeptidase implicated in physiological regulation of processes involving neuropeptides. The present study clarifies the active site structure and mechanism of catalysis of TOP. The enzyme exhibited a bell-shaped pH dependence of activity having an acidic limb du...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00548-9
更新日期:2003-06-19 00:00:00
abstract::The WW domain is known as one of the smallest protein modules with a triple-stranded beta-sheet fold. Here, we present the solution structure of the second WW domain from the mouse salvador homolog 1 protein. This WW domain forms a homodimer with a beta-clam-like motif, as evidenced by size exclusion chromatography, a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.01.008
更新日期:2007-02-06 00:00:00
abstract::A rapidly maturing variant of the red fluorescent protein DsRed was optimized for bacterial expression by random mutagenesis. The brightest variant contains six mutations, two of which (S4T and a silent mutation in codon 2) explain most of the fluorescence enhancement. The novel variants are expressed at 9-60-fold hig...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00856-1
更新日期:2003-09-25 00:00:00
abstract::Phosphorylations catalyzed by cAMP-dependent protein kinase are essential for sperm motility, and type II cAMP-dependent protein kinase in mature sperm has been shown to be firmly bound to the flagellum via the regulatory subunit, RII. The present study documents high-levelled expression of a human, testis-specific RI...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80253-4
更新日期:1989-03-27 00:00:00
abstract::We have investigated the importance of the degree of sialylation when an acute phase glycoprotein, alpha 1-proteinase inhibitor (alpha 1-Pi), was analysed both by affinity chromatography on concanavalin A (Con A)-Sepharose and by crossed immuno-affinoelectrophoresis (CIAE) using Con A in the first dimension. Human alp...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80147-b
更新日期:1990-03-12 00:00:00
abstract::In mammalian cells, the Bcl-2 and Bcl-x(L) proteins suppress programmed cell death whereas the topographically similar Bax protein accelerates the apoptotic process. Recently published data suggest that expression of the human Bax-alpha gene is lethal for the yeast Saccharomyces cerevisiae and that this toxicity can b...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00044-0
更新日期:1996-02-12 00:00:00
abstract::Fibroblast growth factor 11 (FGF11) is an intracellular FGF. Although induction of FGF11 by hypoxia has been observed in several cell types, the molecular function of FGF11 is not clearly understood yet. Here, we investigated the role of FGF11 under hypoxia. We identified hypoxia-inducible factor-1α (HIF-1α) as an int...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12547
更新日期:2017-01-01 00:00:00
abstract::In a previous study [(1987) J. Mol. Biol. 194, 663-673], we isolated ten insertion/deletion mutants (indels) of the maltose binding protein for which the maltose binding constant was only a little or not at all affected. In this paper, we have localized these mutations in the recently solved three-dimensional structur...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(93)81409-s
更新日期:1993-06-28 00:00:00
abstract::Regulation of integrin affinity for its ligand is essential for cell adhesion and migration. Here, we found that direct interaction of vimentin with integrin β1 can enhance binding of integrin α5β1 to its ligand, fibronectin. Conversely, blocking the interaction reduced fibronectin binding, cell migration on a fibrone...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12430
更新日期:2016-10-01 00:00:00