Role of mitochondria and C-terminal membrane anchor of Bcl-2 in Bax induced growth arrest and mortality in Saccharomyces cerevisiae.


:In mammalian cells, the Bcl-2 and Bcl-x(L) proteins suppress programmed cell death whereas the topographically similar Bax protein accelerates the apoptotic process. Recently published data suggest that expression of the human Bax-alpha gene is lethal for the yeast Saccharomyces cerevisiae and that this toxicity can be overcome by co-expressing Bcl-2 or Bcl-x(L). Our findings corroborate these results. However, we find that although Bax induction invariably stops cell growth under all circumstances, it does not lead to death in 'petite' cells. Petites cannot respire because they lack functional mitochondria. It seems that in 'grande' cells, which do possess normal mitochondrial DNA, nutritional limitation is critical for increased mortality. Surprisingly, murine Bcl-2 lacking the membrane anchor of human Bcl-2 has no effect on grande cells, but can efficiently rescue petites in rich medium. It has been suggested that the C-terminal membrane anchor of human Bcl-2 may have a crucial role in rescuing apoptosis in mammalian cells. When murine Bcl-2 is fused to the membrane anchor of yeast mitochondrial Mas70 protein, the Bcl-2 variant mBcl-2-mma rescues not only petites but also grandes, just like human Bcl-x(L). The rescuing ability of Bcl-x(L), which contains its own membrane anchor, surpasses that of mBcl-2-mma. Our results indicate that the process involving Bax-induced growth inhibition followed by possible lethality, and the rescuing effect of Bcl-2 and Bcl-x(L) is linked to yeast mitochondrial function. We propose a model which is consistent with these observations.




FEBS letters


Greenhalf W,Stephan C,Chaudhuri B




Has Abstract


1996-02-12 00:00:00














  • Single-chain precursor of renal gamma-glutamyl transpeptidase.

    abstract::The two subunits of gamma-glutamyl transpeptidase (EC are derived from a single-chain glycosylated precursor. A small fraction of the propeptide survives proteolytic processing in the rat kidney and has been purified by an immunoaffinity technique. The propeptide contains determinants for both the subunits an...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tate SS

    更新日期:1986-01-01 00:00:00

  • Expression of a chimeric, cGMP-sensitive regulatory subunit of the cAMP-dependent protein kinase type I alpha.

    abstract::To study the fluctuations of cGMP in living cells through changes of energy transfer of dissociable fluorescence labeled subunits, we constructed a cGMP-sensitive probe by combining the N-terminus of the type I regulatory subunit of cAMP-dependent protein kinase (PKA) with the cGMP binding sites of cGMP-dependent prot...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wild N,Herberg FW,Hofmann F,Dostmann WR

    更新日期:1995-11-06 00:00:00

  • Arabidopsis thaliana expresses a second functional flavonol synthase.

    abstract::Arabidopsis thaliana L. produces flavonoid pigments, i.e. flavonols, anthocyanidins and proanthocyanidins, from dihydroflavonol substrates. A small family of putative flavonol synthase (FLS) genes had been recognized in Arabidopsis, and functional activity was attributed only to FLS1. Nevertheless, other FLS activitie...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Preuss A,Stracke R,Weisshaar B,Hillebrecht A,Matern U,Martens S

    更新日期:2009-06-18 00:00:00

  • Involvement of nitric oxide in the promotion of cell survival by ceramide 1-phosphate.

    abstract::Macrophages play vital roles in inflammatory responses, and their number at sites of inflammation is strictly regulated by cell death and division. Here, we demonstrate that production of nitric oxide (NO) is a major mechanism whereby ceramide-1-phosphate (C1P) blocks apoptosis in macrophages. However, NO failed to st...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gangoiti P,Granado MH,Arana L,Ouro A,Gómez-Muñoz A

    更新日期:2008-06-25 00:00:00

  • Fatty acid acylation of membrane skeletal proteins in human erythrocytes.

    abstract::Fatty acid acylation of membrane proteins was studied on human erythrocytes by measuring incorporation of [3H]palmitate at different specific radioactivities. A 55 kDa polypeptide within the band 4.5 region was the main acceptor protein for acylation by fatty acids (palmitate, stearate, oleate), while other polypeptid...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Maretzki D,Mariani M,Lutz HU

    更新日期:1990-01-01 00:00:00

  • Presynaptic kainate receptor-mediated facilitation of glutamate release involves Ca2+-calmodulin and PKA in cerebrocortical synaptosomes.

    abstract::We have explored the mechanisms involved in the facilitation of glutamate release mediated by the activation of kainate receptors (KARs) in the cortex using isolated nerve terminals (synaptosomes). Kainate (KA) produced an increase on glutamate release at 100 μM. The effect of KA was antagonized by NBQX (with AMPA rec...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rodríguez-Moreno A,Sihra TS

    更新日期:2013-03-18 00:00:00

  • Adhesion molecules: a new target for immunoliposome-mediated drug delivery.

    abstract::The anti-ICAM-1 monoclonal antibody F10.2 was conjugated to liposomes to target to cells expressing the cell adhesion molecule ICAM-1. We demonstrate that F10.2 immunoliposomes bind to human bronchial epithelial cells (BEAS-2B) and human umbilical vein endothelial cells (HUVEC) in a specific, dose- and time-dependent ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bloemen PG,Henricks PA,van Bloois L,van den Tweel MC,Bloem AC,Nijkamp FP,Crommelin DJ,Storm G

    更新日期:1995-01-03 00:00:00

  • Suppression of Mycobacterium tuberculosis induced reactive oxygen species (ROS) and TNF-alpha mRNA expression in human monocytes by allicin.

    abstract::Chronic inflammation associated with tumor necrosis factor (TNF)-alpha and reactive oxygen species (ROS) is the hallmark of tuberculosis. Mycobacterium tuberculosis (MTB) directly stimulates human monocytes to secrete TNF-alpha. We show the augmented expression of TNF-alpha mRNA in MTB-infected monocytes by cellular a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hasan N,Yusuf N,Toossi Z,Islam N

    更新日期:2006-05-01 00:00:00

  • A distinct 2,5-di-(tert-butyl)-1,4-benzohydroquinone-sensitive calcium store in bovine adrenal chromaffin cells.

    abstract::The fluorescent Ca2+ indicator Fura 2 was used to monitor Ca2+ release induced by the Ins(1,4,5)P3-mobilizing agonist angiotensin II (Ag II), caffeine and 2,5-di-(tert-butyl)-1,4-benzohydroquinone (tuBHQ), in intact bovine adrenal chromaffin cells. Under low external Ca2+ conditions, tuBHQ, Ag II and caffeine elicited...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Robinson IM,Burgoyne RD

    更新日期:1991-09-09 00:00:00

  • Rapid isolation of animal mitochondrial DNA by alkaline extraction.

    abstract::A simple technique for rapid isolation of mitochondrial DNA (mtDNA) from animal cells is described. The method is based on the selective alkaline denaturation procedure of Birnboim and Doly [(1979) Nucleic Acids Res. 7, 1513-1523] and avoids the use of CsCl gradient centrifugation. The yield of mtDNA is comparable to ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Palva TK,Palva ET

    更新日期:1985-11-18 00:00:00

  • Absence of a unique relationship between active transport of lactose and protonmotive force in E. coli.

    abstract::The relationship between active transport of lactose via the lactose permease and the protonmotive force has been determined in E. coli cells using either the respiratory chain inhibitor cyanide or protonophores to decrease the protonmotive force progressively. In contradiction with the prediction of the delocalized c...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ghazi A,Delamourd L,Shechter E

    更新日期:1986-12-15 00:00:00

  • Adiponectin receptor binding proteins--recent advances in elucidating adiponectin signalling pathways.

    abstract::Adiponectin whose systemic levels are reduced in obesity-related diseases ameliorates insulin sensitivity and regulates biological processes like apoptosis, proliferation, migration and inflammation. Adiponectin binds to adiponectin receptors, AdipoR1 and AdipoR2, which are ubiquitously expressed. Clathrin-dependent e...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Buechler C,Wanninger J,Neumeier M

    更新日期:2010-10-22 00:00:00

  • Low-density lipoproteins interact with liposome-binding sites on the cell surface.

    abstract::Under physiological conditions significant amounts of low-density lipoprotein LDL particles ar taken up by cells independently of specific high-affinity LDL receptors (apo-B receptors). Previously it was established that some cells contain surface sites capable of binding liposomes. We proposed that liposome-binding s...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Galkina SI,Ivanov VV,Preobrazhensky SN,Margolis LB,Bergelson LD

    更新日期:1991-08-05 00:00:00

  • Light-dependent delta mu Na-generation and utilization in the marine cyanobacterium Oscillatoria brevis.

    abstract::Light-dependent Na+ and H+ transports, membrane potential (delta psi) and motility have been studied in the cells of the marine cyanobacterium Oscillatoria brevis. In the presence of a protonophorous uncoupler, carbonyl cyanide-m-chlorophenylhydrazone, the intracellular Na+ level is shown to increase in the dark and d...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Brown II,Fadeyev SI,Kirik II,Severina II,Skulachev VP

    更新日期:1990-09-17 00:00:00

  • BMP signaling in vascular diseases.

    abstract::Bone morphogenetic proteins (BMPs) are members of the transforming growth factor-β (TGF-β) family that signal via type I and type II serine/threonine kinase receptors and intracellular Smad transcription factors. BMPs are multifunctional regulators of development and tissue homeostasis and they were initially characte...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Cai J,Pardali E,Sánchez-Duffhues G,ten Dijke P

    更新日期:2012-07-04 00:00:00

  • Effects of substitutions of glycine and asparagine for serine132 on activity and binding of human lipoprotein lipase to very low density lipoproteins.

    abstract::For studying the role of Ser132 in the putative catalytic site of human lipoprotein lipase (LPL), mutant LPL cDNAs expressing LPLs with amino acid substitutions of Gly or Asn for Ser132 were obtained by site-directed mutagenesis, and were expressed in COS-1 cells. Considerable amounts of LPL enzyme protein mass were d...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tashiro J,Kobayashi J,Shirai K,Saito Y,Fukamachi I,Hashimoto H,Nishida T,Shibui T,Morimoto Y,Yoshida S

    更新日期:1992-02-17 00:00:00

  • CITRX thioredoxin is a putative adaptor protein connecting Cf-9 and the ACIK1 protein kinase during the Cf-9/Avr9- induced defence response.

    abstract::Tomato Cf-9, a receptor-like protein (RLP), confers resistance to races of the fungal pathogen Cladosporium fulvum that express the Avr9 avirulence gene. CITRX (Cf-9-interacting thioredoxin) was previously identified in a yeast two-hybrid screen as a protein interacting with the cytoplasmic domain of Cf-9 and shown to...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nekrasov V,Ludwig AA,Jones JD

    更新日期:2006-07-24 00:00:00

  • MicroRNA-145 contributes to enhancing radiosensitivity of cervical cancer cells.

    abstract::In our study, transcriptome microarrays are used to identify differentially expressed miRNAs and mRNAs in cervical cancer specimens. We find that microRNA-145 (miR-145) expression is significantly decreased in cervical cancer tissues and cell lines, and is associated with advanced cancer stages, large tumor size and m...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ye C,Sun NX,Ma Y,Zhao Q,Zhang Q,Xu C,Wang SB,Sun SH,Wang F,Li W

    更新日期:2015-03-12 00:00:00

  • Two sites of primary degradation of the D1-protein induced by acceptor or donor side photo-inhibition in photosystem II core complexes.

    abstract::Depending on experimental conditions we have found that photo-inhibitory treatment of photosystem II (PSII) core complexes, isolated from wheat, can generate two fragments of about 23-24 kDa that contain either the C-terminal or N-terminal regions of the D1-protein. A 24 kDa C-terminal fragment appears when the water ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: De Las Rivas J,Andersson B,Barber J

    更新日期:1992-04-27 00:00:00

  • High homology between a trophoblastic protein (trophoblastin) isolated from ovine embryo and alpha-interferons.

    abstract::Ovine trophoblastic protein B (oTPB), an embryonic protein, is a 20 kDa secretory protein which is synthesized by the ovine conceptus from days 12 to 22 of pregnancy. oTPB was purified by HPLC using ion-exchange chromatography on a DEAE column and was subsequently chromatographed on a reversed-phase column. Automated ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Charpigny G,Reinaud P,Huet JC,Guillomot M,Charlier M,Pernollet JC,Martal J

    更新日期:1988-02-08 00:00:00

  • Purification of a putative Na+/D-glucose cotransporter from pig kidney brush border membranes on a phlorizin affinity column.

    abstract::Phlorizin, a potent inhibitor of the Na+/D-glucose cotransporter, was derivatised to 3-aminophlorizin and subsequently coupled to Affi-Gel 15. Affinity chromatography of pig kidney brush border membranes solubilised in Triton X-100 allowed the purification of a 60 kDa protein on this resin. We consider this protein to...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kitlar T,Morrison AI,Kinne R,Deutscher J

    更新日期:1988-07-04 00:00:00

  • A calorimetric study of Ca2+ binding to two major isotypes of bullfrog parvalbumin.

    abstract::Microcalorimetric titrations of the two major isotypes of parvalbumin (PA1 and PA2) from bullfrog skeletal muscle with Ca2+ in the presence and absence of Mg2+ have been carried out at 25 degrees C and pH 7.0. The observed enthalpy titration curves were analyzed by the least-squares method. The measured enthalpy chang...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tanokura M,Yamada K

    更新日期:1985-06-03 00:00:00

  • Conversion of the sodium channel activator aconitine into a potent alpha 7-selective nicotinic ligand.

    abstract::Methyllycaconitine (MLA) is a competitive antagonist of nicotinic acetylcholine receptors, with a remarkable preference for neuronal [125I]alpha Bgt binding sites. We have begun to investigate the structural basis of its potency and subtype selectivity. MLA is a substituted norditerpenoid alkaloid linked to a 2-(methy...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hardick DJ,Cooper G,Scott-Ward T,Blagbrough IS,Potter BV,Wonnacott S

    更新日期:1995-05-22 00:00:00

  • Identification of putative calcium channels in skeletal muscle microsomes.

    abstract::Saturable binding sites for the labelled calcium antagonist (+/-)[3H]nimodipine were found in guinea-pig hind limb skeletal muscle homogenates. Binding sites were enriched in a microsomal pellet by differential centrifugation of the homogenate. [3H]Nimodipine binding (Kd = 1.5 +/- 0.03 nM, Bmax = 2.1 +/- 0.25 pmol/pro...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ferry DR,Glossmann H

    更新日期:1982-11-08 00:00:00

  • Fluorescence of native and carotenoid-depleted LH2 from Chromatium minutissimum, originating from simultaneous two-photon absorption in the spectral range of the presumed (optically 'dark') S(1) state of carotenoids.

    abstract::Native and carotenoid-depleted peripheral purple bacterial light-harvesting complex (LH2) were investigated by simultaneous two-photon excited (between 1300-1500 nm) fluorescence (TPF). TPF results from direct bacteriochlorophyll excitation in both samples. The spectral position of the 2A(g)(-) state of rhodopin [corr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Krikunova M,Kummrow A,Voigt B,Rini M,Lokstein H,Moskalenko A,Scheer H,Razjivin A,Leupold D

    更新日期:2002-09-25 00:00:00

  • Translational selection shapes codon usage in the GC-rich genome of Chlamydomonas reinhardtii.

    abstract::In unicellular species codon usage is determined by mutational biases and natural selection. Among prokaryotes, the influence of these factors is different if the genome is skewed towards AT or GC, since in AT-rich organisms translational selection is absent. On the other hand, in AT-rich unicellular eukaryotes the tw...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Naya H,Romero H,Carels N,Zavala A,Musto H

    更新日期:2001-07-20 00:00:00

  • Stability engineering of the human antibody repertoire.

    abstract::Human monoclonal antibodies often display limited thermodynamic and colloidal stabilities. This behavior hinders their production, and places limitations on the development of novel formulation conditions and therapeutic applications. Antibodies are highly diverse molecules, with much of the sequence variation observe...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Rouet R,Lowe D,Christ D

    更新日期:2014-01-21 00:00:00

  • 31P NMR as a tool for monitoring detergent solubilization of sarcoplasmic reticulum membranes.

    abstract::Sarcoplasmic reticulum vesicles were solubilized stepwise by the nonionic detergent octaethyleneglycol monododecyl ether; 31P NMR enabled the extent of phospholipid solubilization to be monitored by following the conversion of the broad resonance peak characterizing the phospholipids inserted in the bilayer to the nar...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Roux M,Champeil P

    更新日期:1984-06-11 00:00:00

  • Comparison of the current RefSeq, Ensembl and EST databases for counting genes and gene discovery.

    abstract::Large amounts of refined sequence material in the form of predicted, curated and annotated genes and expressed sequences tags (ESTs) have recently been added to the NCBI databases. We matched the transcript-sequences of RefSeq, Ensembl and dbEST in an attempt to provide an updated overview of how many unique human gen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Larsson TP,Murray CG,Hill T,Fredriksson R,Schiöth HB

    更新日期:2005-01-31 00:00:00

  • ATP-dependent degradation of SulA, a cell division inhibitor, by the HslVU protease in Escherichia coli.

    abstract::HslVU is an ATP-dependent protease consisting of two multimeric components, the HslU ATPase and the HslV peptidase. To gain an insight into the role of HslVU in regulation of cell division, the reconstituted enzyme was incubated with SulA, an inhibitor of cell division in Escherichia coli, or its fusion protein with m...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Seong IS,Oh JY,Yoo SJ,Seol JH,Chung CH

    更新日期:1999-07-30 00:00:00