Role of mitochondria and C-terminal membrane anchor of Bcl-2 in Bax induced growth arrest and mortality in Saccharomyces cerevisiae.

abstract：:The two subunits of gamma-glutamyl transpeptidase (EC 2.3.2.2) are derived from a single-chain glycosylated precursor. A small fraction of the propeptide survives proteolytic processing in the rat kidney and has been purified by an immunoaffinity technique. The propeptide contains determinants for both the subunits an...

journal_title：FEBS letters

authors： Tate SS

更新日期：1986-01-01 00:00:00

abstract：:To study the fluctuations of cGMP in living cells through changes of energy transfer of dissociable fluorescence labeled subunits, we constructed a cGMP-sensitive probe by combining the N-terminus of the type I regulatory subunit of cAMP-dependent protein kinase (PKA) with the cGMP binding sites of cGMP-dependent prot...

journal_title：FEBS letters

authors： Wild N,Herberg FW,Hofmann F,Dostmann WR

更新日期：1995-11-06 00:00:00

abstract：:Arabidopsis thaliana L. produces flavonoid pigments, i.e. flavonols, anthocyanidins and proanthocyanidins, from dihydroflavonol substrates. A small family of putative flavonol synthase (FLS) genes had been recognized in Arabidopsis, and functional activity was attributed only to FLS1. Nevertheless, other FLS activitie...

journal_title：FEBS letters

authors： Preuss A,Stracke R,Weisshaar B,Hillebrecht A,Matern U,Martens S

更新日期：2009-06-18 00:00:00

abstract：:Macrophages play vital roles in inflammatory responses, and their number at sites of inflammation is strictly regulated by cell death and division. Here, we demonstrate that production of nitric oxide (NO) is a major mechanism whereby ceramide-1-phosphate (C1P) blocks apoptosis in macrophages. However, NO failed to st...

journal_title：FEBS letters

authors： Gangoiti P,Granado MH,Arana L,Ouro A,Gómez-Muñoz A

更新日期：2008-06-25 00:00:00

abstract：:Fatty acid acylation of membrane proteins was studied on human erythrocytes by measuring incorporation of [3H]palmitate at different specific radioactivities. A 55 kDa polypeptide within the band 4.5 region was the main acceptor protein for acylation by fatty acids (palmitate, stearate, oleate), while other polypeptid...

journal_title：FEBS letters

authors： Maretzki D,Mariani M,Lutz HU

更新日期：1990-01-01 00:00:00

abstract：:We have explored the mechanisms involved in the facilitation of glutamate release mediated by the activation of kainate receptors (KARs) in the cortex using isolated nerve terminals (synaptosomes). Kainate (KA) produced an increase on glutamate release at 100 μM. The effect of KA was antagonized by NBQX (with AMPA rec...

journal_title：FEBS letters

authors： Rodríguez-Moreno A,Sihra TS

更新日期：2013-03-18 00:00:00

abstract：:The anti-ICAM-1 monoclonal antibody F10.2 was conjugated to liposomes to target to cells expressing the cell adhesion molecule ICAM-1. We demonstrate that F10.2 immunoliposomes bind to human bronchial epithelial cells (BEAS-2B) and human umbilical vein endothelial cells (HUVEC) in a specific, dose- and time-dependent ...

journal_title：FEBS letters

authors： Bloemen PG,Henricks PA,van Bloois L,van den Tweel MC,Bloem AC,Nijkamp FP,Crommelin DJ,Storm G

更新日期：1995-01-03 00:00:00

abstract：:Chronic inflammation associated with tumor necrosis factor (TNF)-alpha and reactive oxygen species (ROS) is the hallmark of tuberculosis. Mycobacterium tuberculosis (MTB) directly stimulates human monocytes to secrete TNF-alpha. We show the augmented expression of TNF-alpha mRNA in MTB-infected monocytes by cellular a...

journal_title：FEBS letters

authors： Hasan N,Yusuf N,Toossi Z,Islam N

更新日期：2006-05-01 00:00:00

abstract：:The fluorescent Ca2+ indicator Fura 2 was used to monitor Ca2+ release induced by the Ins(1,4,5)P3-mobilizing agonist angiotensin II (Ag II), caffeine and 2,5-di-(tert-butyl)-1,4-benzohydroquinone (tuBHQ), in intact bovine adrenal chromaffin cells. Under low external Ca2+ conditions, tuBHQ, Ag II and caffeine elicited...

journal_title：FEBS letters

authors： Robinson IM,Burgoyne RD

更新日期：1991-09-09 00:00:00

abstract：:A simple technique for rapid isolation of mitochondrial DNA (mtDNA) from animal cells is described. The method is based on the selective alkaline denaturation procedure of Birnboim and Doly [(1979) Nucleic Acids Res. 7, 1513-1523] and avoids the use of CsCl gradient centrifugation. The yield of mtDNA is comparable to ...

journal_title：FEBS letters

authors： Palva TK,Palva ET

更新日期：1985-11-18 00:00:00

abstract：:The relationship between active transport of lactose via the lactose permease and the protonmotive force has been determined in E. coli cells using either the respiratory chain inhibitor cyanide or protonophores to decrease the protonmotive force progressively. In contradiction with the prediction of the delocalized c...

journal_title：FEBS letters

authors： Ghazi A,Delamourd L,Shechter E

更新日期：1986-12-15 00:00:00

abstract：:Adiponectin whose systemic levels are reduced in obesity-related diseases ameliorates insulin sensitivity and regulates biological processes like apoptosis, proliferation, migration and inflammation. Adiponectin binds to adiponectin receptors, AdipoR1 and AdipoR2, which are ubiquitously expressed. Clathrin-dependent e...

journal_title：FEBS letters

authors： Buechler C,Wanninger J,Neumeier M

更新日期：2010-10-22 00:00:00

abstract：:Under physiological conditions significant amounts of low-density lipoprotein LDL particles ar taken up by cells independently of specific high-affinity LDL receptors (apo-B receptors). Previously it was established that some cells contain surface sites capable of binding liposomes. We proposed that liposome-binding s...

journal_title：FEBS letters

authors： Galkina SI,Ivanov VV,Preobrazhensky SN,Margolis LB,Bergelson LD

更新日期：1991-08-05 00:00:00

abstract：:Light-dependent Na+ and H+ transports, membrane potential (delta psi) and motility have been studied in the cells of the marine cyanobacterium Oscillatoria brevis. In the presence of a protonophorous uncoupler, carbonyl cyanide-m-chlorophenylhydrazone, the intracellular Na+ level is shown to increase in the dark and d...

journal_title：FEBS letters

authors： Brown II,Fadeyev SI,Kirik II,Severina II,Skulachev VP

更新日期：1990-09-17 00:00:00

abstract：:Bone morphogenetic proteins (BMPs) are members of the transforming growth factor-β (TGF-β) family that signal via type I and type II serine/threonine kinase receptors and intracellular Smad transcription factors. BMPs are multifunctional regulators of development and tissue homeostasis and they were initially characte...

journal_title：FEBS letters

authors： Cai J,Pardali E,Sánchez-Duffhues G,ten Dijke P

更新日期：2012-07-04 00:00:00

abstract：:For studying the role of Ser132 in the putative catalytic site of human lipoprotein lipase (LPL), mutant LPL cDNAs expressing LPLs with amino acid substitutions of Gly or Asn for Ser132 were obtained by site-directed mutagenesis, and were expressed in COS-1 cells. Considerable amounts of LPL enzyme protein mass were d...

journal_title：FEBS letters

authors： Tashiro J,Kobayashi J,Shirai K,Saito Y,Fukamachi I,Hashimoto H,Nishida T,Shibui T,Morimoto Y,Yoshida S

更新日期：1992-02-17 00:00:00

abstract：:Tomato Cf-9, a receptor-like protein (RLP), confers resistance to races of the fungal pathogen Cladosporium fulvum that express the Avr9 avirulence gene. CITRX (Cf-9-interacting thioredoxin) was previously identified in a yeast two-hybrid screen as a protein interacting with the cytoplasmic domain of Cf-9 and shown to...

journal_title：FEBS letters

authors： Nekrasov V,Ludwig AA,Jones JD

更新日期：2006-07-24 00:00:00

abstract：:In our study, transcriptome microarrays are used to identify differentially expressed miRNAs and mRNAs in cervical cancer specimens. We find that microRNA-145 (miR-145) expression is significantly decreased in cervical cancer tissues and cell lines, and is associated with advanced cancer stages, large tumor size and m...

journal_title：FEBS letters

authors： Ye C,Sun NX,Ma Y,Zhao Q,Zhang Q,Xu C,Wang SB,Sun SH,Wang F,Li W

更新日期：2015-03-12 00:00:00

abstract：:Depending on experimental conditions we have found that photo-inhibitory treatment of photosystem II (PSII) core complexes, isolated from wheat, can generate two fragments of about 23-24 kDa that contain either the C-terminal or N-terminal regions of the D1-protein. A 24 kDa C-terminal fragment appears when the water ...

journal_title：FEBS letters

authors： De Las Rivas J,Andersson B,Barber J

更新日期：1992-04-27 00:00:00

abstract：:Ovine trophoblastic protein B (oTPB), an embryonic protein, is a 20 kDa secretory protein which is synthesized by the ovine conceptus from days 12 to 22 of pregnancy. oTPB was purified by HPLC using ion-exchange chromatography on a DEAE column and was subsequently chromatographed on a reversed-phase column. Automated ...

journal_title：FEBS letters

authors： Charpigny G,Reinaud P,Huet JC,Guillomot M,Charlier M,Pernollet JC,Martal J

更新日期：1988-02-08 00:00:00

abstract：:Phlorizin, a potent inhibitor of the Na+/D-glucose cotransporter, was derivatised to 3-aminophlorizin and subsequently coupled to Affi-Gel 15. Affinity chromatography of pig kidney brush border membranes solubilised in Triton X-100 allowed the purification of a 60 kDa protein on this resin. We consider this protein to...

journal_title：FEBS letters

authors： Kitlar T,Morrison AI,Kinne R,Deutscher J

更新日期：1988-07-04 00:00:00

abstract：:Microcalorimetric titrations of the two major isotypes of parvalbumin (PA1 and PA2) from bullfrog skeletal muscle with Ca2+ in the presence and absence of Mg2+ have been carried out at 25 degrees C and pH 7.0. The observed enthalpy titration curves were analyzed by the least-squares method. The measured enthalpy chang...

journal_title：FEBS letters

authors： Tanokura M,Yamada K

更新日期：1985-06-03 00:00:00

abstract：:Methyllycaconitine (MLA) is a competitive antagonist of nicotinic acetylcholine receptors, with a remarkable preference for neuronal [125I]alpha Bgt binding sites. We have begun to investigate the structural basis of its potency and subtype selectivity. MLA is a substituted norditerpenoid alkaloid linked to a 2-(methy...

journal_title：FEBS letters

authors： Hardick DJ,Cooper G,Scott-Ward T,Blagbrough IS,Potter BV,Wonnacott S

更新日期：1995-05-22 00:00:00

abstract：:Saturable binding sites for the labelled calcium antagonist (+/-)[3H]nimodipine were found in guinea-pig hind limb skeletal muscle homogenates. Binding sites were enriched in a microsomal pellet by differential centrifugation of the homogenate. [3H]Nimodipine binding (Kd = 1.5 +/- 0.03 nM, Bmax = 2.1 +/- 0.25 pmol/pro...

journal_title：FEBS letters

authors： Ferry DR,Glossmann H

更新日期：1982-11-08 00:00:00

abstract：:Native and carotenoid-depleted peripheral purple bacterial light-harvesting complex (LH2) were investigated by simultaneous two-photon excited (between 1300-1500 nm) fluorescence (TPF). TPF results from direct bacteriochlorophyll excitation in both samples. The spectral position of the 2A(g)(-) state of rhodopin [corr...

journal_title：FEBS letters

authors： Krikunova M,Kummrow A,Voigt B,Rini M,Lokstein H,Moskalenko A,Scheer H,Razjivin A,Leupold D

更新日期：2002-09-25 00:00:00

abstract：:In unicellular species codon usage is determined by mutational biases and natural selection. Among prokaryotes, the influence of these factors is different if the genome is skewed towards AT or GC, since in AT-rich organisms translational selection is absent. On the other hand, in AT-rich unicellular eukaryotes the tw...

journal_title：FEBS letters

authors： Naya H,Romero H,Carels N,Zavala A,Musto H

更新日期：2001-07-20 00:00:00

abstract：:Human monoclonal antibodies often display limited thermodynamic and colloidal stabilities. This behavior hinders their production, and places limitations on the development of novel formulation conditions and therapeutic applications. Antibodies are highly diverse molecules, with much of the sequence variation observe...

journal_title：FEBS letters

authors： Rouet R,Lowe D,Christ D

更新日期：2014-01-21 00:00:00

abstract：:Sarcoplasmic reticulum vesicles were solubilized stepwise by the nonionic detergent octaethyleneglycol monododecyl ether; 31P NMR enabled the extent of phospholipid solubilization to be monitored by following the conversion of the broad resonance peak characterizing the phospholipids inserted in the bilayer to the nar...

journal_title：FEBS letters

authors： Roux M,Champeil P

更新日期：1984-06-11 00:00:00

abstract：:Large amounts of refined sequence material in the form of predicted, curated and annotated genes and expressed sequences tags (ESTs) have recently been added to the NCBI databases. We matched the transcript-sequences of RefSeq, Ensembl and dbEST in an attempt to provide an updated overview of how many unique human gen...

journal_title：FEBS letters

authors： Larsson TP,Murray CG,Hill T,Fredriksson R,Schiöth HB

更新日期：2005-01-31 00:00:00

abstract：:HslVU is an ATP-dependent protease consisting of two multimeric components, the HslU ATPase and the HslV peptidase. To gain an insight into the role of HslVU in regulation of cell division, the reconstituted enzyme was incubated with SulA, an inhibitor of cell division in Escherichia coli, or its fusion protein with m...

journal_title：FEBS letters

authors： Seong IS,Oh JY,Yoo SJ,Seol JH,Chung CH

更新日期：1999-07-30 00:00:00