Role of mitochondria and C-terminal membrane anchor of Bcl-2 in Bax induced growth arrest and mortality in Saccharomyces cerevisiae.


:In mammalian cells, the Bcl-2 and Bcl-x(L) proteins suppress programmed cell death whereas the topographically similar Bax protein accelerates the apoptotic process. Recently published data suggest that expression of the human Bax-alpha gene is lethal for the yeast Saccharomyces cerevisiae and that this toxicity can be overcome by co-expressing Bcl-2 or Bcl-x(L). Our findings corroborate these results. However, we find that although Bax induction invariably stops cell growth under all circumstances, it does not lead to death in 'petite' cells. Petites cannot respire because they lack functional mitochondria. It seems that in 'grande' cells, which do possess normal mitochondrial DNA, nutritional limitation is critical for increased mortality. Surprisingly, murine Bcl-2 lacking the membrane anchor of human Bcl-2 has no effect on grande cells, but can efficiently rescue petites in rich medium. It has been suggested that the C-terminal membrane anchor of human Bcl-2 may have a crucial role in rescuing apoptosis in mammalian cells. When murine Bcl-2 is fused to the membrane anchor of yeast mitochondrial Mas70 protein, the Bcl-2 variant mBcl-2-mma rescues not only petites but also grandes, just like human Bcl-x(L). The rescuing ability of Bcl-x(L), which contains its own membrane anchor, surpasses that of mBcl-2-mma. Our results indicate that the process involving Bax-induced growth inhibition followed by possible lethality, and the rescuing effect of Bcl-2 and Bcl-x(L) is linked to yeast mitochondrial function. We propose a model which is consistent with these observations.




FEBS letters


Greenhalf W,Stephan C,Chaudhuri B




Has Abstract


1996-02-12 00:00:00














  • Expression of serum amyloid A transcripts in human trophoblast and fetal-derived trophoblast-like choriocarcinoma cells.

    abstract::The placenta comprises a highly specialized trophoblast layer, which arises from the embryo and differentiates during embryonic development to perform specialized functions, e.g., synthesis of pregnancy-associated hormones, growth factors and cytokines. As there is no evidence of maternal acute-phase protein transplac...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kovacevic A,Hammer A,Sundl M,Pfister B,Hrzenjak A,Ray A,Ray BK,Sattler W,Malle E

    更新日期:2006-01-09 00:00:00

  • Evidence for a role of glycosphingolipids in CXCR4-dependent cell migration.

    abstract::Chemotaxis induction is a major effect evoked by stimulation of the chemokine receptor CXCR4 with its sole ligand CXCL12. We now report that treatment of CHP-100 human neuroepithelioma cells with the glucosylceramide synthase (GCS) inhibitor DL-threo-1-phenyl-2-hexadecanoylamino-3-pyrrolidino-1-propanol inhibits CXCR4...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Limatola C,Massa V,Lauro C,Catalano M,Giovanetti A,Nuccitelli S,Spinedi A

    更新日期:2007-06-12 00:00:00

  • Weak pulling forces exerted on Nin-orientated transmembrane segments during co-translational insertion into the inner membrane of Escherichia coli.

    abstract::Transmembrane helices (TMHs) in membrane proteins can be orientated with their N-terminus towards the cytoplasm (Nin), or facing the non-cytoplasmic side (Nout). Most membrane proteins are inserted co-translationally into membranes, aided by Sec-type translocons. Since the final orientation of Nin- and Nout-orientated...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cymer F,Ismail N,von Heijne G

    更新日期:2014-05-21 00:00:00

  • Structural identification of the lipopolysaccharide-binding capability of a cupin-family protein from Helicobacter pylori.

    abstract::We solved the crystal structure of a functionally uncharacterized protein, HP0902, from Helicobacter pylori. Its structure demonstrated an all-β cupin fold that cannot bind metal ions due to the absence of a metal-binding histidine that is conserved in many metallo-cupins. In contrast, isothermal titration calorimetry...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Sim DW,Kim JH,Kim HY,Jang JH,Lee WC,Kim EH,Park PJ,Lee KH,Won HS

    更新日期:2016-09-01 00:00:00

  • Subunit exchange of lens alpha-crystallin: a fluorescence energy transfer study with the fluorescent labeled alphaA-crystallin mutant W9F as a probe.

    abstract::A Trp-free alphaA-crystallin mutant (W9F) was prepared by site-directed mutation. This mutant appears to be identical to the wild-type in terms of conformation (secondary and tertiary structures). W9F was labeled with a sulfhydryl-specific fluorescent probe, 2-(4'-maleimidylanilino) naphthalene-6-sulfonate (MIANS), an...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sun TX,Akhtar NJ,Liang JJ

    更新日期:1998-07-03 00:00:00

  • The environments of Trp-248 and Trp-330 in tryptophan indole-lyase from Escherichia coli.

    abstract::The two tryptophan residues, Trp-248 and Trp-330, in tryptophan indole-lyase (tryptophanase) from E. coli have been separately mutated to phenylalanine using site-directed mutagenesis. Both single tryptophan mutant enzymes have full catalytic activity, but exhibit different fluorescence and near-UV circular dichroism ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Phillips RS,Gollnick P

    更新日期:1990-07-30 00:00:00

  • Synthesis of phage-specific transfer RNA molecules by vibriophage phi 149.

    abstract::32P-Labelled tRNA was isolated from uninfected and phage phi 149-infected Vibrio cholerae cells. These tRNA preparations were then hybridised with DNA isolated from phage phi 149. Significant hybridisation was observed only with tRNA from phage phi 149-infected cells. This strongly suggests that infection of classical...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ghosh RK,Guhathakurta I

    更新日期:1983-10-03 00:00:00

  • Permeation and gating of alpha1 glycine-gated channels expressed at low and high density in Xenopus oocyte.

    abstract::When a high density of alpha1-subunit glycine receptor (GlyR) is expressed in Xenopus oocytes, two populations of channels can be distinguished according to their apparent affinity for glycine which differs 5- to 6-fold. To compare the open pore diameter of these channels, the relative permeability of formate with res...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Maammar M,Rodeau JL,Taleb O

    更新日期:1997-09-01 00:00:00

  • Stimulation of nuclear sphingosine kinase activity by platelet-derived growth factor.

    abstract::Subcellular fractionation revealed that a significant fraction of total sphingosine kinase, the enzyme that phosphorylates sphingosine to form the bioactive lipid metabolite sphingosine-1-phosphate, resides in the nuclei of Swiss 3T3 cells, localized to both the nuclear envelope and the nucleoplasm. Platelet-derived g...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kleuser B,Maceyka M,Milstien S,Spiegel S

    更新日期:2001-08-10 00:00:00

  • The 20S proteasome gene family in Arabidopsis thaliana.

    abstract::The complexity of the proteasome gene family in higher plants was investigated by identification and sequencing cDNA clones from the Arabidopsis thaliana database showing homologies to 20S proteasome subunits. We identified plant counterparts for each of the 14 proteasomal subunit subfamilies. Moreover, several of the...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Parmentier Y,Bouchez D,Fleck J,Genschik P

    更新日期:1997-10-27 00:00:00

  • Oligodeoxynucleotide analogs with 5'-linked anthraquinone.

    abstract::We report here the synthesis of novel 5'-linked oligodeoxynucleotides, both normal phosphodiester and phosphorothioate analogs, in which a covalently attached group at the 5'-terminus is an anthraquinone. These compounds represent a new class of antisense compounds in which the base sequence of the oligodeoxynucleotid...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mori K,Subasinghe C,Cohen JS

    更新日期:1989-06-05 00:00:00

  • The carbon and energy sources of the non-photosynthetic plastid in the malaria parasite.

    abstract::The malaria parasite harbours an indispensable plastid known as the 'apicoplast'. The apicoplast's exact role remains uncertain, but it houses components involved in fatty acid, isoprenoid and haem biosyntheses. These pathways offer opportunities to develop anti-malarials. In the absence of photosynthesis, how apicopl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lim L,Linka M,Mullin KA,Weber AP,McFadden GI

    更新日期:2010-02-05 00:00:00

  • Mitochondrial dysfunction and nuclear-mitochondrial shuttling of TERT are involved in cell proliferation arrest induced by G-quadruplex ligands.

    abstract::G-quadruplex ligands DODC and TMPyP4 have different binding modes to quadruplex structure and cause cell proliferation arrest. Here we showed that DODC was more efficient in cell growth inhibition than TMPyP4. Both G-quadruplex ligands induced nuclear-cytoplasmic shuttling and accumulation of TERT in mitochondria. Thi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zhuang XY,Yao YG

    更新日期:2013-06-05 00:00:00

  • Transbilayer reorientation of platelet-activating factor in the erythrocyte membrane.

    abstract::Platelet-activating factor (PAF) is a phospholipid, intermediate in its structure between diacyl- and lysophospholipids. The reorientation of this highly bioactive compound to the inner membrane layer of human erythrocytes after its primary incorporation into the outer membrane layer has been studied. Reorientation wa...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schneider E,Haest CW,Deuticke B

    更新日期:1986-03-31 00:00:00

  • Interferon gamma-dependent transactivation of epidermal growth factor receptor.

    abstract::The present report provides evidence that, in A431 cells, interferon gamma (IFNgamma) induces the rapid (within 5 min), and reversible, tyrosine phosphorylation of the epidermal growth factor receptor (EGFR). IFNgamma-induced EGFR transactivation requires EGFR kinase activity, as well as activity of the Src-family tyr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Burova E,Vassilenko K,Dorosh V,Gonchar I,Nikolsky N

    更新日期:2007-04-03 00:00:00

  • Solubilization of sarcoplasmic reticulum membranes by sodium dodecylsulphate. A Fourier-transform infrared spectroscopic study.

    abstract::In order to improve our understanding of membrane protein solubilization by sodium dodecylsulphate, sarcoplasmic reticulum vesicles have been treated with this surfactant at different detergent: protein mole ratios. Effects on Ca2(+)-ATPase activity, membrane protein solubilization, and protein conformation have been ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Prado A,Muga A,Castresana J,Goñi FM,Arrondo JL

    更新日期:1990-09-03 00:00:00

  • Transcription of the halophage phi H repressor gene is abolished by transcription from an inversely oriented lytic promoter.

    abstract::The temperate phage phi H of the extremely halophilic archaebacterium Halobacterium salinarium encodes a repressor, Rep, which in the immune state represses the production of an early lytic transcript, denoted T4. Rep acts at the transcriptional level by blocking the promoter for T4. The promoter for the rep gene itse...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Stolt P,Zillig W

    更新日期:1994-05-16 00:00:00

  • Purification and characterization of phospholipase A2 inhibitory proteins from pig thyroid gland.

    abstract::A 32 kDa phospholipase A2 inhibitory protein was isolated from pig thyroid gland after calcium precipitation and fast protein liquid anion-exchange chromatography. SDS-polyacrylamide gel electrophoresis revealed the purity of the protein. The protein activity was assessed by the inhibition of pancreatic phospholipase ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Antonicelli F,Rothhut B,Martiny L,Aguie-Aguie G,Lambert B,Bellon G,Russo-Marie F,Jacquemin C,Haye B

    更新日期:1988-08-01 00:00:00

  • Disulfide bridge reorganization induced by proline mutations in maurotoxin.

    abstract::Maurotoxin (MTX) is a 34-residue toxin that has been isolated from the venom of the chactidae scorpion Scorpio maurus palmatus, and characterized. Together with Pi1 and HsTx1, MTX belongs to a family of short-chain four-disulfide-bridged scorpion toxins acting on potassium channels. However, contrary to other members ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Carlier E,Fajloun Z,Mansuelle P,Fathallah M,Mosbah A,Oughideni R,Sandoz G,Di Luccio E,Geib S,Regaya I,Brocard J,Rochat H,Darbon H,Devaux C,Sabatier JM,de Waard M

    更新日期:2001-02-02 00:00:00

  • Order and disorder in 30 nm chromatin fibers.

    abstract::The tendency of DNA to form fibers upon condensation with counterions is reviewed. It is shown that chromatin fibers may acquire a relatively constant diameter of about 30 nm simply as an optimal size achieved upon neutralization of DNA, without requiring a repetitive internal structure. Thus the size of chromatin fib...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Subirana JA

    更新日期:1992-05-11 00:00:00

  • Studies on the primary sequence requirements for PKC-alpha, -beta 1 and -gamma peptide substrates.

    abstract::The substrate specificity of purified PKC-alpha, -beta and -gamma has been investigated. A series of synthetic peptides based upon the sequence surrounding serine-7 in glycogen synthase were generated and used to determine the basic residue requirements of these PKC isotypes. While PKC-alpha and -beta are indistinguis...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Marais RM,Nguyen O,Woodgett JR,Parker PJ

    更新日期:1990-12-17 00:00:00

  • Human small cell lung cancer NYH cells resistant to the bisdioxopiperazine ICRF-187 exhibit a functional dominant Tyr165Ser mutation in the Walker A ATP binding site of topoisomerase II alpha.

    abstract::Bisdioxopiperazine anti-cancer agents are catalytic inhibitors of topoisomerase II which by unknown means lock the enzyme in a closed clamp form and inhibit its ATPase activity. In order to demarcate a putative pharmacophore, we here describe a novel Tyr165Ser mutation in the enzyme's Walker A ATP binding site leading...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wessel I,Jensen LH,Renodon-Corniere A,Sorensen TK,Nitiss JL,Jensen PB,Sehested M

    更新日期:2002-06-05 00:00:00

  • Ether stress-induced Alzheimer-like tau phosphorylation in the normal mouse brain.

    abstract::Tau is reversibly hyperphosphorylated in the mouse brain by starvation or cold water swimming. Here, we report tau phosphorylation in the hippocampus of normal mouse after ether anesthesia, known to trigger typical stress reactions. Robust phosphorylation of tau was observed immediately and 10min after ether vapor exp...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ikeda Y,Ishiguro K,Fujita SC

    更新日期:2007-03-06 00:00:00

  • Geometry of binding of the N alpha-tosylated piperidides of m-amidino-, p-amidino- and p-guanidino phenylalanine to thrombin and trypsin. X-ray crystal structures of their trypsin complexes and modeling of their thrombin complexes.

    abstract::The X-ray crystal structures of the complexes formed with bovine trypsin and the N alpha-tosylated piperidides of m-amidino-, p-amidino- and p-guanidino-D,L-phenylalanine (3-TAPAP, 4-TAPAP and 4-TGPAP) were determined with data to 1.8 A resolution. The L-stereoisomer of 3-TAPAP binds as a compact entity into the activ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Turk D,Stürzebecher J,Bode W

    更新日期:1991-08-05 00:00:00

  • Down-regulation of the protein kinase A pathway by activators of protein kinase C and intracellular Ca2+ in fibroblast cells.

    abstract::Many genes are regulated by the intracellular calcium, protein kinase C (PKC) and protein kinase A (PKA) pathways and it has been shown that these pathways synergize in some cell types, whereas they antagonize in others. Here we show that the calcium and PKC pathways suppress the effects mediated by the PKA pathway in...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Döbbeling U,Berchtold MW

    更新日期:1996-08-05 00:00:00

  • The directionality of processive enzymes acting on recalcitrant polysaccharides is reflected in the kinetic signatures of oligomer degradation.

    abstract::The enzymatic degradation of the closely related insoluble polysaccharides; cellulose (β(1-4)-linked glucose) by cellulases and chitin (β(1-4)-linked N-acetylglucosamine) by chitinases, is of large biological and economical importance. Processive enzymes with different inherent directionalities, i.e. attacking the pol...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hamre AG,Schaupp D,Eijsink VG,Sørlie M

    更新日期:2015-07-08 00:00:00

  • Ce-wts-1 plays important roles in Caenorhabditis elegans development.

    abstract::The Hippo-Warts pathway defines a novel signaling cascade involved in organ size control and tumor suppression. However, the developmental function of this pathway is less understood. Here we report that the Caenorhabditis elegans homolog of Warts, Ce-wts-1, plays important roles during worm development. The null alle...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cai Q,Wang W,Gao Y,Yang Y,Zhu Z,Fan Q

    更新日期:2009-10-06 00:00:00

  • Two excited states in aequorin bioluminescence induced by tryptophan modification.

    abstract::The Ca(2+)-activated photoprotein, aequorin, contains six tryptophan residues and has a bioluminescence emission maximum at 465 nm. On converting the six tryptophan residues to phenylalanine, the mutant aequorins exhibited varied luminescence activities and spectra, but one mutant, with tryptophan-86 replaced by pheny...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ohmiya Y,Ohashi M,Tsuji FI

    更新日期:1992-04-20 00:00:00

  • Excitation delocalization over the whole core antenna of photosynthetic purple bacteria evidenced by non-linear pump-probe spectroscopy.

    abstract::Anomalously high values of photoinduced absorption changes were revealed in the antenna of photosynthetic purple bacteria. They were found to be 4-16 times greater at the bleaching peak of the antenna than at the bleaching peak of the BChl dimer of the reaction center. This is direct proof of excitation delocalization...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Novoderezhkin VI,Razjivin AP

    更新日期:1995-07-17 00:00:00

  • Benzophenone synthase from cultured cells of Centaurium erythraea.

    abstract::A central step in xanthone biosynthesis is the formation of the C13 skeleton, i.e. an intermediate benzophenone. Biosynthesis of 2,3',4,6-tetrahydroxybenzophenone from m-hydroxybenzoyl-CoA and malonyl-CoA was shown in cell-free extracts from cultured cells of Centaurium erythraea. The enzyme catalyzing this reaction w...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Beerhues L

    更新日期:1996-04-01 00:00:00