Role of mitochondria and C-terminal membrane anchor of Bcl-2 in Bax induced growth arrest and mortality in Saccharomyces cerevisiae.

abstract：:The placenta comprises a highly specialized trophoblast layer, which arises from the embryo and differentiates during embryonic development to perform specialized functions, e.g., synthesis of pregnancy-associated hormones, growth factors and cytokines. As there is no evidence of maternal acute-phase protein transplac...

journal_title：FEBS letters

authors： Kovacevic A,Hammer A,Sundl M,Pfister B,Hrzenjak A,Ray A,Ray BK,Sattler W,Malle E

更新日期：2006-01-09 00:00:00

abstract：:Chemotaxis induction is a major effect evoked by stimulation of the chemokine receptor CXCR4 with its sole ligand CXCL12. We now report that treatment of CHP-100 human neuroepithelioma cells with the glucosylceramide synthase (GCS) inhibitor DL-threo-1-phenyl-2-hexadecanoylamino-3-pyrrolidino-1-propanol inhibits CXCR4...

journal_title：FEBS letters

authors： Limatola C,Massa V,Lauro C,Catalano M,Giovanetti A,Nuccitelli S,Spinedi A

更新日期：2007-06-12 00:00:00

abstract：:Transmembrane helices (TMHs) in membrane proteins can be orientated with their N-terminus towards the cytoplasm (Nin), or facing the non-cytoplasmic side (Nout). Most membrane proteins are inserted co-translationally into membranes, aided by Sec-type translocons. Since the final orientation of Nin- and Nout-orientated...

journal_title：FEBS letters

authors： Cymer F,Ismail N,von Heijne G

更新日期：2014-05-21 00:00:00

abstract：:We solved the crystal structure of a functionally uncharacterized protein, HP0902, from Helicobacter pylori. Its structure demonstrated an all-β cupin fold that cannot bind metal ions due to the absence of a metal-binding histidine that is conserved in many metallo-cupins. In contrast, isothermal titration calorimetry...

journal_title：FEBS letters

authors： Sim DW,Kim JH,Kim HY,Jang JH,Lee WC,Kim EH,Park PJ,Lee KH,Won HS

更新日期：2016-09-01 00:00:00

abstract：:A Trp-free alphaA-crystallin mutant (W9F) was prepared by site-directed mutation. This mutant appears to be identical to the wild-type in terms of conformation (secondary and tertiary structures). W9F was labeled with a sulfhydryl-specific fluorescent probe, 2-(4'-maleimidylanilino) naphthalene-6-sulfonate (MIANS), an...

journal_title：FEBS letters

authors： Sun TX,Akhtar NJ,Liang JJ

更新日期：1998-07-03 00:00:00

abstract：:The two tryptophan residues, Trp-248 and Trp-330, in tryptophan indole-lyase (tryptophanase) from E. coli have been separately mutated to phenylalanine using site-directed mutagenesis. Both single tryptophan mutant enzymes have full catalytic activity, but exhibit different fluorescence and near-UV circular dichroism ...

journal_title：FEBS letters

authors： Phillips RS,Gollnick P

更新日期：1990-07-30 00:00:00

abstract：:32P-Labelled tRNA was isolated from uninfected and phage phi 149-infected Vibrio cholerae cells. These tRNA preparations were then hybridised with DNA isolated from phage phi 149. Significant hybridisation was observed only with tRNA from phage phi 149-infected cells. This strongly suggests that infection of classical...

journal_title：FEBS letters

authors： Ghosh RK,Guhathakurta I

更新日期：1983-10-03 00:00:00

abstract：:When a high density of alpha1-subunit glycine receptor (GlyR) is expressed in Xenopus oocytes, two populations of channels can be distinguished according to their apparent affinity for glycine which differs 5- to 6-fold. To compare the open pore diameter of these channels, the relative permeability of formate with res...

journal_title：FEBS letters

authors： Maammar M,Rodeau JL,Taleb O

更新日期：1997-09-01 00:00:00

abstract：:Subcellular fractionation revealed that a significant fraction of total sphingosine kinase, the enzyme that phosphorylates sphingosine to form the bioactive lipid metabolite sphingosine-1-phosphate, resides in the nuclei of Swiss 3T3 cells, localized to both the nuclear envelope and the nucleoplasm. Platelet-derived g...

journal_title：FEBS letters

authors： Kleuser B,Maceyka M,Milstien S,Spiegel S

更新日期：2001-08-10 00:00:00

abstract：:The complexity of the proteasome gene family in higher plants was investigated by identification and sequencing cDNA clones from the Arabidopsis thaliana database showing homologies to 20S proteasome subunits. We identified plant counterparts for each of the 14 proteasomal subunit subfamilies. Moreover, several of the...

journal_title：FEBS letters

authors： Parmentier Y,Bouchez D,Fleck J,Genschik P

更新日期：1997-10-27 00:00:00

abstract：:We report here the synthesis of novel 5'-linked oligodeoxynucleotides, both normal phosphodiester and phosphorothioate analogs, in which a covalently attached group at the 5'-terminus is an anthraquinone. These compounds represent a new class of antisense compounds in which the base sequence of the oligodeoxynucleotid...

journal_title：FEBS letters

authors： Mori K,Subasinghe C,Cohen JS

更新日期：1989-06-05 00:00:00

abstract：:The malaria parasite harbours an indispensable plastid known as the 'apicoplast'. The apicoplast's exact role remains uncertain, but it houses components involved in fatty acid, isoprenoid and haem biosyntheses. These pathways offer opportunities to develop anti-malarials. In the absence of photosynthesis, how apicopl...

journal_title：FEBS letters

authors： Lim L,Linka M,Mullin KA,Weber AP,McFadden GI

更新日期：2010-02-05 00:00:00

abstract：:G-quadruplex ligands DODC and TMPyP4 have different binding modes to quadruplex structure and cause cell proliferation arrest. Here we showed that DODC was more efficient in cell growth inhibition than TMPyP4. Both G-quadruplex ligands induced nuclear-cytoplasmic shuttling and accumulation of TERT in mitochondria. Thi...

journal_title：FEBS letters

authors： Zhuang XY,Yao YG

更新日期：2013-06-05 00:00:00

abstract：:Platelet-activating factor (PAF) is a phospholipid, intermediate in its structure between diacyl- and lysophospholipids. The reorientation of this highly bioactive compound to the inner membrane layer of human erythrocytes after its primary incorporation into the outer membrane layer has been studied. Reorientation wa...

journal_title：FEBS letters

authors： Schneider E,Haest CW,Deuticke B

更新日期：1986-03-31 00:00:00

abstract：:The present report provides evidence that, in A431 cells, interferon gamma (IFNgamma) induces the rapid (within 5 min), and reversible, tyrosine phosphorylation of the epidermal growth factor receptor (EGFR). IFNgamma-induced EGFR transactivation requires EGFR kinase activity, as well as activity of the Src-family tyr...

journal_title：FEBS letters

authors： Burova E,Vassilenko K,Dorosh V,Gonchar I,Nikolsky N

更新日期：2007-04-03 00:00:00

abstract：:In order to improve our understanding of membrane protein solubilization by sodium dodecylsulphate, sarcoplasmic reticulum vesicles have been treated with this surfactant at different detergent: protein mole ratios. Effects on Ca2(+)-ATPase activity, membrane protein solubilization, and protein conformation have been ...

journal_title：FEBS letters

authors： Prado A,Muga A,Castresana J,Goñi FM,Arrondo JL

更新日期：1990-09-03 00:00:00

abstract：:The temperate phage phi H of the extremely halophilic archaebacterium Halobacterium salinarium encodes a repressor, Rep, which in the immune state represses the production of an early lytic transcript, denoted T4. Rep acts at the transcriptional level by blocking the promoter for T4. The promoter for the rep gene itse...

journal_title：FEBS letters

authors： Stolt P,Zillig W

更新日期：1994-05-16 00:00:00

abstract：:A 32 kDa phospholipase A2 inhibitory protein was isolated from pig thyroid gland after calcium precipitation and fast protein liquid anion-exchange chromatography. SDS-polyacrylamide gel electrophoresis revealed the purity of the protein. The protein activity was assessed by the inhibition of pancreatic phospholipase ...

journal_title：FEBS letters

authors： Antonicelli F,Rothhut B,Martiny L,Aguie-Aguie G,Lambert B,Bellon G,Russo-Marie F,Jacquemin C,Haye B

更新日期：1988-08-01 00:00:00

abstract：:Maurotoxin (MTX) is a 34-residue toxin that has been isolated from the venom of the chactidae scorpion Scorpio maurus palmatus, and characterized. Together with Pi1 and HsTx1, MTX belongs to a family of short-chain four-disulfide-bridged scorpion toxins acting on potassium channels. However, contrary to other members ...

journal_title：FEBS letters

authors： Carlier E,Fajloun Z,Mansuelle P,Fathallah M,Mosbah A,Oughideni R,Sandoz G,Di Luccio E,Geib S,Regaya I,Brocard J,Rochat H,Darbon H,Devaux C,Sabatier JM,de Waard M

更新日期：2001-02-02 00:00:00

abstract：:The tendency of DNA to form fibers upon condensation with counterions is reviewed. It is shown that chromatin fibers may acquire a relatively constant diameter of about 30 nm simply as an optimal size achieved upon neutralization of DNA, without requiring a repetitive internal structure. Thus the size of chromatin fib...

journal_title：FEBS letters

authors： Subirana JA

更新日期：1992-05-11 00:00:00

abstract：:The substrate specificity of purified PKC-alpha, -beta and -gamma has been investigated. A series of synthetic peptides based upon the sequence surrounding serine-7 in glycogen synthase were generated and used to determine the basic residue requirements of these PKC isotypes. While PKC-alpha and -beta are indistinguis...

journal_title：FEBS letters

authors： Marais RM,Nguyen O,Woodgett JR,Parker PJ

更新日期：1990-12-17 00:00:00

abstract：:Bisdioxopiperazine anti-cancer agents are catalytic inhibitors of topoisomerase II which by unknown means lock the enzyme in a closed clamp form and inhibit its ATPase activity. In order to demarcate a putative pharmacophore, we here describe a novel Tyr165Ser mutation in the enzyme's Walker A ATP binding site leading...

journal_title：FEBS letters

authors： Wessel I,Jensen LH,Renodon-Corniere A,Sorensen TK,Nitiss JL,Jensen PB,Sehested M

更新日期：2002-06-05 00:00:00

abstract：:Tau is reversibly hyperphosphorylated in the mouse brain by starvation or cold water swimming. Here, we report tau phosphorylation in the hippocampus of normal mouse after ether anesthesia, known to trigger typical stress reactions. Robust phosphorylation of tau was observed immediately and 10min after ether vapor exp...

journal_title：FEBS letters

authors： Ikeda Y,Ishiguro K,Fujita SC

更新日期：2007-03-06 00:00:00

abstract：:The X-ray crystal structures of the complexes formed with bovine trypsin and the N alpha-tosylated piperidides of m-amidino-, p-amidino- and p-guanidino-D,L-phenylalanine (3-TAPAP, 4-TAPAP and 4-TGPAP) were determined with data to 1.8 A resolution. The L-stereoisomer of 3-TAPAP binds as a compact entity into the activ...

journal_title：FEBS letters

authors： Turk D,Stürzebecher J,Bode W

更新日期：1991-08-05 00:00:00

abstract：:Many genes are regulated by the intracellular calcium, protein kinase C (PKC) and protein kinase A (PKA) pathways and it has been shown that these pathways synergize in some cell types, whereas they antagonize in others. Here we show that the calcium and PKC pathways suppress the effects mediated by the PKA pathway in...

journal_title：FEBS letters

authors： Döbbeling U,Berchtold MW

更新日期：1996-08-05 00:00:00

abstract：:The enzymatic degradation of the closely related insoluble polysaccharides; cellulose (β(1-4)-linked glucose) by cellulases and chitin (β(1-4)-linked N-acetylglucosamine) by chitinases, is of large biological and economical importance. Processive enzymes with different inherent directionalities, i.e. attacking the pol...

journal_title：FEBS letters

authors： Hamre AG,Schaupp D,Eijsink VG,Sørlie M

更新日期：2015-07-08 00:00:00

abstract：:The Hippo-Warts pathway defines a novel signaling cascade involved in organ size control and tumor suppression. However, the developmental function of this pathway is less understood. Here we report that the Caenorhabditis elegans homolog of Warts, Ce-wts-1, plays important roles during worm development. The null alle...

journal_title：FEBS letters

authors： Cai Q,Wang W,Gao Y,Yang Y,Zhu Z,Fan Q

更新日期：2009-10-06 00:00:00

abstract：:The Ca(2+)-activated photoprotein, aequorin, contains six tryptophan residues and has a bioluminescence emission maximum at 465 nm. On converting the six tryptophan residues to phenylalanine, the mutant aequorins exhibited varied luminescence activities and spectra, but one mutant, with tryptophan-86 replaced by pheny...

journal_title：FEBS letters

authors： Ohmiya Y,Ohashi M,Tsuji FI

更新日期：1992-04-20 00:00:00

abstract：:Anomalously high values of photoinduced absorption changes were revealed in the antenna of photosynthetic purple bacteria. They were found to be 4-16 times greater at the bleaching peak of the antenna than at the bleaching peak of the BChl dimer of the reaction center. This is direct proof of excitation delocalization...

journal_title：FEBS letters

authors： Novoderezhkin VI,Razjivin AP

更新日期：1995-07-17 00:00:00

abstract：:A central step in xanthone biosynthesis is the formation of the C13 skeleton, i.e. an intermediate benzophenone. Biosynthesis of 2,3',4,6-tetrahydroxybenzophenone from m-hydroxybenzoyl-CoA and malonyl-CoA was shown in cell-free extracts from cultured cells of Centaurium erythraea. The enzyme catalyzing this reaction w...

journal_title：FEBS letters

authors： Beerhues L

更新日期：1996-04-01 00:00:00