Abstract:
:The study of the three-dimensional organization of chromatin has recently gained much focus in the context of novel techniques for detecting genome-wide contacts using next-generation sequencing. These chromosome conformation capture-based methods give a deep topological insight into the architecture of the genome inside the nucleus. Several recent studies observe a compartmentalization of chromatin interactions into spatially confined domains. This structural feature of interphase chromosomes is not only supported by conventional studies assessing the interaction data of millions of cells, but also by analysis on the level of a single cell. We first present and examine the different models that have been proposed to elucidate these topological domains in eukaryotes. Then we show that a model which relies on the dynamic formation of loops within domains can account for the experimentally observed contact maps. Interestingly, the topological domain structure is not only found in mammalian genomes, but also in bacterial chromosomes.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Hofmann A,Heermann DWdoi
10.1016/j.febslet.2015.04.021subject
Has Abstractpub_date
2015-10-07 00:00:00pages
2958-65issue
20 Pt Aeissn
0014-5793issn
1873-3468pii
S0014-5793(15)00259-8journal_volume
589pub_type
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