Abstract:
:The pluripotent nature of human embryonic stem cells (hESC) has attracted great interest in using them as a source of cells or tissue in cell therapy. However, in order to be used in regenerative medicine, the pluripotent hESC lines should be established and propagated according to good manufacturing practice quality requirements. The cultures should be animal substance free in order to exclude the risk of infections and immunogenity. They should also be genetically and epigenetically normal. The detailed molecular mechanisms of their pluripotency are still not defined. Using human feeder cells, a medium containing only human proteins, the mechanical isolation of the inner cell mass and mechanical passaging of hESC, is a safe option until a functional defined medium containing physiological concentrations of regulatory factors is available.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Skottman H,Dilber MS,Hovatta Odoi
10.1016/j.febslet.2006.03.083subject
Has Abstractpub_date
2006-05-22 00:00:00pages
2875-8issue
12eissn
0014-5793issn
1873-3468pii
S0014-5793(06)00410-8journal_volume
580pub_type
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