Abstract:
:Antibodies were raised, in rabbits, against an arachidonate- and U46619-binding protein purified from calf platelets. Spectral measurements and immunodiffusion experiments were employed to follow conformational responses of the protein in relation to platelet activation. Upon treatment with the platelet agonists, arachidonate and PGH2, as well as the common haem ligands, imidazole and CN-, the purified protein had its Soret band red-shifted, with hypochromicity, but the protein saturated with the agonists, not with the haem ligands, showed altered antigenic properties in immunodiffusion experiments. In an analogous manner activation of gel-filtered calf platelets with high concentrations of ADP and A23187, as well as by cold, had Soret bands of extracts of sonicated platelets red-shifted, with hypochromicity; concomitantly, antigenically different conformations of the protein appeared in Triton X-100 extracts of the activated platelets. A protein immunologically related to the platelet protein was detected in Triton X-100 extracts of calf neutrophils. It is suggested that conformational changes of the protein induced by arachidonate or prostaglandin endoperoxides or H2O2 formed in different compartments during platelet activation by different stimuli may be a biochemical mechanism of stimulus-response coupling and that similar mechanisms might operate in other cell types.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Krishnan LK,Jamaluddin MPdoi
10.1016/0014-5793(87)81347-9subject
Has Abstractpub_date
1987-02-23 00:00:00pages
213-9issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(87)81347-9journal_volume
212pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The roles of 2'-OH groups in the binding of mRNA to human ribosomes were studied using site-directed cross-linking. We found that both mRNA and mDNA analogues bearing a cross-linker can modify ribosomal proteins (rps) S3e and S2e at the mRNA entry site independently on tRNA presence, but only mRNA analogues were capab...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.09.003
更新日期:2012-10-19 00:00:00
abstract::Spectral variants of the green fluorescent protein (GFP) have been extensively used as reporters to image molecular interactions in living cells by fluorescence resonance energy transfer (FRET). However, those GFP variants which are the most efficient donor acceptor pairs for FRET measurements show a high degree of sp...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03508-1
更新日期:2002-11-06 00:00:00
abstract::We synthesized the zwitterionic amphiphile cholesterylphosphorylethylpyridinium. This substance activated protein kinase C (PKC) in a micelle-based assay, but in a vesicle assay it was inhibitory. An analog of this compound, in which the pyridine ring is saturated and the nitrogen methylated, showed similar behaviour ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80629-u
更新日期:1992-06-15 00:00:00
abstract::The regulation by insulin and carbohydrates of the gene expression of three key enzymes involved in glucose metabolism was studied in the liver of the Wistar fatty rat, a model of obese non-insulin-dependent diabetes mellitus. A high glucose or fructose diet, or insulin administration caused a similar magnitude of inc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80982-z
更新日期:1993-08-09 00:00:00
abstract::Members of the Trp protein family have been suggested as the structural basis of store-operated cation conductances. With this study, we provide evidence for the expression of three isoforms of Trp (hTrp1, 3 and 4) in human umbilical vein endothelial cells (HUVEC). The role of Trp proteins in store regulation of endot...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01212-5
更新日期:1998-10-16 00:00:00
abstract::The absorption frequencies of the C = O and C = C (neutral state) and of the C...O (semiquinone state) stretching vibrations of QB have been assigned by FTIR spectroscopy, using native and site-specifically 1-, 2-, 3- and 4-13C-labelled ubiquinone-10 (UQ10) reconstituted at the QB binding site of Rhodobacter sphaeroid...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00805-j
更新日期:1995-08-14 00:00:00
abstract::The monoclonal antibody 791T /36, prepared against a human osteogenic sarcoma cell line, 791T , reacts with a variety of human tumours and also mitogen-stimulated PBMN cells. The target antigen as expressed upon 791T cells is a monomeric plasma membrane-associated glycoprotein with an apparent Mr of 72000. By quantita...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80454-8
更新日期:1984-06-04 00:00:00
abstract::Activated p38gamma MAP kinase exhibited significant basal ATPase activity in the absence of a kinase substrate, and addition of a phosphoacceptor substrate increased k(cat)/K(m)20-fold. AMP-PCP was competitive with ATP binding and non-competitive with phosphoacceptor substrate binding. The nucleotide binding site affi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01488-x
更新日期:1999-11-19 00:00:00
abstract::13CO NMR chemical shifts and 12CO infrared stretching frequencies have been measured for cytochrome P-450cam-CO in the presence of D-camphor and of various camphor analogues. A linear correlation between both parameters delta (13C) and v(CO) was found indicating that the steric and electrostatic interactions acting on...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00377-l
更新日期:1995-05-08 00:00:00
abstract::The covalently closed circular DNA (cccDNA) of hepatitis B virus (HBV) is a viral center molecule for HBV infection and persistence. However, the cellular restriction factors of HBV cccDNA are not well understood. Here, we show that TGF-β can induce nuclear viral cccDNA degradation and hypermutation via activation-ind...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12058
更新日期:2016-02-01 00:00:00
abstract::The sialic acid of complex N-glycans can be biochemically engineered by substituting the physiological precursor N-acetylmannosamine with non-natural N-acylmannosamines. The Kv3.1 glycoprotein, a neuronal voltage-gated potassium channel, contains sialic acid. Western blots of the Kv3.1 glycoprotein isolated from trans...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.09.021
更新日期:2011-10-20 00:00:00
abstract::In this study, we have identified 876 polymorphism sites in 145 complete or partial genomes of SARS-CoV available in the NCBI GenBank. One hundred and seventy-four of these sites existed in two or more SARS-CoV genome sequences. According to the sequence polymorphism, all SARS-CoVs can be divided into three groups: (I...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.07.075
更新日期:2005-09-12 00:00:00
abstract::Amyloid precursor protein (APP) gene expression was investigated in primary cultures of neurons, astrocytes, microglial cells and oligodendrocytes. Neurons from various rat brain regions, as well as oligodendrocytes, contained RNA encoding APP695, while astrocytes and microglial cells expressed high levels of RNAs for...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80706-m
更新日期:1992-08-03 00:00:00
abstract::Activator protein 1 (AP1) is a complex of Fos and Jun, and it regulates the transcription of genes possessing the AP1-binding sequence. The purpose of this study was to detect living cells that express AP1 after stimulation with a tumor promoter. The Fos and Jun components of AP1 were induced rapidly and transiently i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00487-5
更新日期:1996-06-10 00:00:00
abstract::Bovine tryptophanyl-tRNA synthetase (TrpRS, E.C.6.1.1.2) is unable to catalyze in vitro formation of Ap4A in contrast to some other aminoacyl-tRNA synthetases. However, in the presence of L-tryptophan, ATP-Mg2+ and ADP the enzyme catalyzes the Ap3A synthesis via adenylate intermediate. Ap3A (not Ap4A) may serve as a s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00764-0
更新日期:1994-08-22 00:00:00
abstract::Advanced glycation end products (AGE) are known to serve as ligands for the scavenger receptors such as SR-A, CD36 and SR-BI. In the current study, we examined whether AGE is recognized by lectin-like oxidized low density lipoprotein receptor-1 (LOX-1). Cellular binding experiments revealed that AGE-bovine serum album...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03325-7
更新日期:2002-01-30 00:00:00
abstract::We solved the crystal structure of a functionally uncharacterized protein, HP0902, from Helicobacter pylori. Its structure demonstrated an all-β cupin fold that cannot bind metal ions due to the absence of a metal-binding histidine that is conserved in many metallo-cupins. In contrast, isothermal titration calorimetry...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12332
更新日期:2016-09-01 00:00:00
abstract::Mammalian color vision is mediated by light-sensitive pigments in retinal cone cells. Biochemical studies on native mammalian cone visual pigments are seriously hampered by their low levels and instability. We describe a novel approach for their functional expression, employing the baculovirus system in combination wi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)01064-2
更新日期:1996-10-28 00:00:00
abstract::The hepatitis B viral X protein (HBx) is a multifunctional protein that plays a role in the activation of the NF-kappaB signaling pathway. In this study, we demonstrate that HBx up-regulates expression of TANK-binding kinase-1 (TBK1) at the transcriptional level. Increased levels of TBK1 protein then induce the phosph...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.11.091
更新日期:2010-02-05 00:00:00
abstract::By analyzing human embryonic kidney 293 cell transfectants stably overexpressing various types of phospholipase A2 (PLA2), we have shown that polyunsaturated fatty acids (PUFAs) preferentially activate type IIA secretory PLA2 (sPLA2-IIA)-mediated arachidonic acid (AA) release from interleukin-1 (IL-1)-stimulated cells...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00702-4
更新日期:1999-06-18 00:00:00
abstract::Molybdenum is an essential micronutrient required by plants. The mechanism of molybdenum uptake in plants is poorly understood, however, evidence has suggested that sulfate transporters may be involved. The sulfate transporter from Stylosanthes hamata, SHST1, restored growth of the sulfate transport yeast mutant, YSD1...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.03.045
更新日期:2008-04-30 00:00:00
abstract::Titin is a giant protein which extends from Z-line to M-line in striated muscles. We report here the purification of a 150-kDa titin fragment, obtained after V8 protease treatment of myofibrils. This polypeptide was located at the N1-line level, in a titin part known to exhibit stiff properties correlated to an associ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00572-9
更新日期:1998-06-05 00:00:00
abstract::We report for the first time the identification of 25 microRNAs from tissues originating from chicken embryo and adult chicken. Most of the cloned microRNAs are expressed in both adult chickens and chicken embryos. Fourteen were identified without any prior prediction. One microRNA, miR-757, is thought to be chicken-s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.05.044
更新日期:2006-06-26 00:00:00
abstract::The acronym CK2 denotes a highly pleiotropic Ser/Thr protein kinase whose over-expression correlates with neoplastic growth. A vexed question about the enigmatic regulation of CK2 concerns the actual existence in living cells of the catalytic (alpha and/or alpha') and regulatory beta-subunits of CK2 not assembled into...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.06.031
更新日期:2006-07-10 00:00:00
abstract::This study describes evidence for the absorption of flavonoids and their presence in human plasma in the glycosylated form by HPLC analysis with photodiode array detection. Rutin and other quercetin glycosides, phloridzin, as well as an anthocyanin are detected simultaneously. In addition, a compound eluting with the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01442-1
更新日期:1997-01-13 00:00:00
abstract::Sperm beta-acrosin activity is inhibited by suramin, a polysulfonated naphthylurea compound with therapeutic potential as a combined antifertility agent and microbicide. A kinetic analysis of enzyme inhibition suggests that three and four molecules of suramin bind to one molecule of ram and boar beta-acrosins respecti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00488-5
更新日期:2003-06-05 00:00:00
abstract::In rat brain astroglia-enriched cultures long-term treatment with interleukin-1beta induces NO release and stimulation of soluble guanylyl cyclase. The cGMP formed is recovered in the extracellular medium but not in the cell monolayer. The interleukin-1beta effect is mediated by type I receptor and potentiated by inte...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03003-4
更新日期:2001-11-02 00:00:00
abstract::The aim of this study was to investigate the distribution of a calcium-inhibitable adenylyl cyclase type VI (type VI AC) in the central nervous system using an antiserum directed against the N-terminus of type VI AC. Our results indicate that type VI AC immunoreactivity is generally expressed in many brain regions wit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01098-9
更新日期:1998-09-25 00:00:00
abstract::Mouse embryonic stem (ES) cells homozygous for disruption of the MSK1 gene had no detectable MSK1 activity. However, their activators (extracellular signal related kinase (ERK)1/ERK2) were stimulated normally in mitogen- and stress-activated protein kinase (MSK)1-/- and wild type cells in response to tetradecanoylphor...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02031-7
更新日期:2000-09-29 00:00:00
abstract::Fructose 2,6-bisphosphate has been claimed to be both a substrate analogue and an allosteric inhibitor of fructose-1,6-bisphosphatase. The results reported here show that fructose 2,6-bisphosphate can be both an inhibitor and an activator of the enzyme, depending on the substrate concentration. This biphasic behaviour...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80126-x
更新日期:1984-02-27 00:00:00