Abstract:
:Members of the Trp protein family have been suggested as the structural basis of store-operated cation conductances. With this study, we provide evidence for the expression of three isoforms of Trp (hTrp1, 3 and 4) in human umbilical vein endothelial cells (HUVEC). The role of Trp proteins in store regulation of endothelial membrane conductances was tested by expression of an N-terminal fragment of hTrp3 (N-TRP) which exerts a dominant negative effect on Trp channel function presumably due to suppression of channel assembly. Depletion of intracellular Ca2+ stores with IP3 (100 microM) or thapsigargin (100 nM) induced a substantial cation conductance in sham-transfected HUVEC as well as in HUVEC transfected with hTrp3. In contrast, HUVEC transfected with N-TRP failed to exhibit store-operated currents. Our results suggest the involvement of Trp related proteins in the store-operated cation conductance of human vascular endothelial cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Groschner K,Hingel S,Lintschinger B,Balzer M,Romanin C,Zhu X,Schreibmayer Wdoi
10.1016/s0014-5793(98)01212-5subject
Has Abstractpub_date
1998-10-16 00:00:00pages
101-6issue
1-2eissn
0014-5793issn
1873-3468pii
S0014-5793(98)01212-5journal_volume
437pub_type
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