Mutational analysis of the role of calcium ions in the Lactobacillus reuteri strain 121 fructosyltransferase (levansucrase and inulosucrase) enzymes.

abstract：:Macrophage cyclooxygenase-2 (COX-2) transcription is mediated through the collaboration of different promoter elements. Here, the role of an overlapping cyclic AMP responsive element (CRE)/E-box was investigated. Nuclear proteins bound both the CRE and E-box, which synergized with other promoter elements to induce COX...

journal_title：FEBS letters

authors： Mestre JR,Rivadeneira DE,Mackrell PJ,Duff M,Stapleton PP,Mack-Strong V,Maddali S,Smyth GP,Tanabe T,Daly JM

更新日期：2001-05-11 00:00:00

abstract：:The cytochrome bc1 complex is a key component of the mitochondrial respiratory chains of many eukaryotic microorganisms that are pathogenic for plants or humans, such as fungi responsible for crop diseases and Plasmodium falciparum, which causes human malaria. Cytochrome bc1 is an enzyme that contains two (ubi)quinone...

journal_title：FEBS letters

authors： Fisher N,Meunier B,Biagini GA

更新日期：2020-09-01 00:00:00

abstract：:The less polar fluorescent analogue of ATP, 2'(3')-trinitrophenyl-5'-triphosphate bound to rat liver fatty acid-binding protein with high affinity (Kd 6.3 x 10(-6) M) and 1:1 molar stoichiometry. This probe bound to the fatty acid binding site of the protein and was displaced by oleic acid and oleoyl CoA. High concent...

journal_title：FEBS letters

authors： Sheridan M,Wilton DC

更新日期：1992-12-21 00:00:00

abstract：:The Methanocaldococcus jannaschii (formerly Methanococcus jannaschii) protein Mj0968 has been reported to represent a soluble P-type ATPase [Ogawa et al., FEBS Lett. 471 (2000) 99-102]. In this study, we report that the heterologously expressed Mj0968-His(10) protein exhibits high rates of phosphatase activity, wherea...

journal_title：FEBS letters

authors： Bramkamp M,Gassel M,Herkenhoff-Hesselmann B,Bertrand J,Altendorf K

更新日期：2003-05-22 00:00:00

abstract：:A novel GTP binding protein (G protein) alpha subunit cDNA was isolated from a T cell leukemia cell line, Jurkat, utilizing polymerase chain reaction (PCR). The predicted amino acid sequence of this G protein alpha subunit showed the highest identity (96.6%) to bovine cone cell-specific transducin (Tc alpha). The orga...

journal_title：FEBS letters

authors： Kubo M,Hirano T,Kakinuma M

更新日期：1991-10-21 00:00:00

abstract：:Horseradish peroxidase in the presence of hydrogen peroxide (HRP/H2O2) oxidizes a carcinogenic non-aminoazo dye, 1-phenylazo-2-hydroxynaphthalene (Sudan I) to the ultimate carcinogen, which binds to calf thymus DNA. The principal product of Sudan I oxidation by the HRP/H2O2 system is the benzenediazonium ion. Minor pr...

journal_title：FEBS letters

authors： Stiborová M,Asfaw B,Anzenbacher P

更新日期：1988-05-23 00:00:00

abstract：:Rats bearing the Yoshida AH-130 ascites hepatoma showed an increased expression of both uncoupling protein-2 (UCP2) (194%) and UCP3 (189%) mRNA levels in skeletal muscle 7 days after tumour inoculation. Interestingly, an even greater increase was observed in mRNA for both UCP2 (278%) and UCP3 (797%) in the pair-fed an...

journal_title：FEBS letters

authors： Sanchís D,Busquets S,Alvarez B,Ricquier D,López-Soriano FJ,Argilés JM

更新日期：1998-10-09 00:00:00

abstract：:Leonor Michaelis spent the years of 1922-1926 as Professor of Biochemistry of the Aichi Medical College (now Graduate School of Medicine, Nagoya University) in Nagoya, Japan. Michaelis succeeded in gathering many bright young biochemists from all over Japan into his laboratory, and made tremendous contributions to the...

journal_title：FEBS letters

authors： Nagatsu TT

更新日期：2013-09-02 00:00:00

abstract：:So far, various approaches for phylogenetic analysis have been developed. Almost all of them put stress on analyzing nucleic acid sequences or protein primary structures. In this paper, we take the physicochemical properties of amino acids into account and introduce the hydropathy profile of amino acids into phylogene...

journal_title：FEBS letters

authors： Liu N,Wang T

更新日期：2006-10-02 00:00:00

abstract：:The oxidoreductase ERp57 is a component of the major histocompatibility complex (MHC) class I peptide-loading complex. ERp57 can interact directly with MHC class I molecules, however, little is known about which of the cysteine residues within the MHC class I molecule are relevant to this interaction. MHC class I mole...

journal_title：FEBS letters

authors： Antoniou AN,Santos SG,Campbell EC,Lynch S,Arosa FA,Powis SJ

更新日期：2007-05-15 00:00:00

abstract：:The concept of critical period plasticity in rat visual cortex has been studied in terms of changes in the level of filamentous actin and of the 200 kDa neurofilament polypeptide. Our results suggest that the postnatal developmental profile of filamentous actin is affected by visual experience, as a consequence of eye...

journal_title：FEBS letters

authors： Nona SN,Trowell SC,Cronly-Dillon JR

更新日期：1985-07-01 00:00:00

abstract：:Heat shock (44 degrees C for 10 min) or ATP depletion by an uncoupler (CCCP for 20 min) is shown to result in stimulation of hsp68/70 synthesis in Ehrlich tumor cells. After 3 h of recovery, the cells become thermotolerant. Surprisingly, repeated ATP depletion caused by CCCP or rotenone (a respiratory inhibitor) treat...

journal_title：FEBS letters

authors： Gabai VL,Kabakov AE

更新日期：1993-08-02 00:00:00

abstract：:cDNAs encoding the isoform I alpha of the cGMP-dependent protein kinase were isolated from a bovine trachea smooth muscle cDNA library constructed in lambda gt10. The deduced protein sequence is identical with the protein sequence obtained by Edman degradation of the bovine lung enzyme [(1984) Biochemistry 23, 4207-42...

journal_title：FEBS letters

authors： Wernet W,Flockerzi V,Hofmann F

更新日期：1989-07-17 00:00:00

abstract：:The effects of nine drugs on the CD spectra of a synthetic calcium binding analog of site III of rabbit skeletal troponin C, can generally be divided into 3 groups: (1) that consisting of haloperidol, benperidol, molindone and promethazine, had no effect on the CD spectrum or calcium sensitivity of the apopeptide; (2)...

journal_title：FEBS letters

authors： Reid RE,Gariépy J,Hodges RS

更新日期：1983-04-05 00:00:00

abstract：:We used a xenograft model to investigate whether the aryl hydrocarbon receptor deletion construct CDelta553 suppresses tumor growth. HeLa cells that were infected with CDelta553 expressing adenovirus (Ad553) formed very small tumors whereas the control adenovirus-infected cells formed large tumors at day 15. CDelta553...

journal_title：FEBS letters

authors： Wang D,Faridi JS,Li Y,Chan WK

更新日期：2009-09-17 00:00:00

abstract：:Heterotrimeric G proteins were purified from bovine brain by immunoaffinity chromatography on immobilized anti G protein monoclonal antibody 3C2. Release of betagamma subunits was effectuated by exposure of immobilized trimeric G proteins to MgAlF4. The resultant betagamma subunits were pure and biologically active. F...

journal_title：FEBS letters

authors： Rehm A,Ploegh HL

更新日期：1997-02-03 00:00:00

abstract：:The multidrug resistance protein (MRP) is an ATP-dependent transport protein for organic anions, as well as neutral or positively charged anticancer agents. In this study we report that dinitrophenyl-S-glutathione increases ATPase activity in plasma membrane vesicles prepared from the MRP-overexpressing cell line GLC4...

journal_title：FEBS letters

authors： Hooijberg JH,Broxterman HJ,Heijn M,Fles DL,Lankelma J,Pinedo HM

更新日期：1997-08-18 00:00:00

abstract：:A single protein from human leukocytes possesses both 5-lipoxygenase and leukotriene A4 (LTA4) synthase activities. It has been reported that LTA4 production is more efficient when the enzyme utilizes arachidonic acid, than when 5-HPETE is exogenously supplied as substrate. In the present study, human leukocyte homoge...

journal_title：FEBS letters

authors： Puustinen T,Scheffer MM,Samuelsson B

更新日期：1987-06-15 00:00:00

abstract：:(-)-Epigallocatechin-3-O-gallate (EGCG) exhibits anti-tumor activity mediated via the 67-kDa laminin receptor (67LR). In this study, we found that 67LR protein levels are reduced by exposure to low O(2) levels (5%), without affecting the expression of HIF-1α. We also found that EGCG-induced anti-cancer activity is abr...

journal_title：FEBS letters

authors： Tsukamoto S,Yamashita S,Kim YH,Kumazoe M,Huang Y,Yamada K,Tachibana H

更新日期：2012-09-21 00:00:00

abstract：:The photoreduction of the quinone (Q) pool in the photosynthetic membrane of the purple bacterium Rhodobacter sphaeroides was investigated by steady-state and time-resolved Fourier transform infrared difference spectroscopy. The results are consistent with the existence of a homogeneous Q pool inside the chromatophore...

journal_title：FEBS letters

authors： Mezzetti A,Leibl W,Breton J,Nabedryk E

更新日期：2003-02-27 00:00:00

abstract：:We have investigated whether the expression in Arabidopsis thaliana seeds of a transgene (the Phaseolus vulgaris arcelin (arc)5-I gene) could be enhanced by the simultaneous introduction of an antisense gene for an endogenous seed storage protein (2S albumin). Seeds of plants transformed with both the arc5-I gene and ...

journal_title：FEBS letters

authors： Goossens A,Van Montagu M,Angenon G

更新日期：1999-07-30 00:00:00

abstract：:We have followed with autoradiography the course of DNA synthesis and with cytofluorometry the partition of DNA during mitosis, through the different phases of the life-span of human fibroblasts. Towards the end of the proliferative potential of the cell population, DNA synthesis was disturbed during the late part of ...

journal_title：FEBS letters

authors： Macieira-Coelho A

更新日期：1995-01-23 00:00:00

abstract：:Chimeric proteins composed of ricin toxin A chain (RTA) and staphylococcal protein A (PA) have been produced in E. coli. Constructs consisting of N-terminal RTA and C-terminal PA (RTA-PA) or N-terminal PA and C-terminal (PA-RTA) were capable of binding to immunoglobulin G (via PA) and of specifically depurinating 28 S...

journal_title：FEBS letters

authors： O'Hare M,Brown AN,Hussain K,Gebhardt A,Watson G,Roberts LM,Vitetta ES,Thorpe PE,Lord JM

更新日期：1990-10-29 00:00:00

abstract：:Rat liver fructose 1,6-bisphosphatase can be protected against partial inactivation by N-ethylmaleimide by low concentrations of fructose 2,6-bisphosphate or high concentrations of fructose 1,6-bisphosphate. The partially inactivated enzyme has a much reduced sensitivity to high substrate inhibition and has lost the s...

journal_title：FEBS letters

authors： Meek DW,Nimmo HG

更新日期：1983-08-22 00:00:00

abstract：:The rate of whole cell H2O2 metabolism in several salivarian and stercorarian trypanosomes and Leishmania species was measured. These cells metabolized H2O2 at rates between 2.3 and 48.2 nmol/10(8) cells per min depending upon the species employed. H2O2 metabolism was largely insensitive to NaN3, implying that typical...

journal_title：FEBS letters

authors： Penketh PG,Kennedy WP,Patton CL,Sartorelli AC

更新日期：1987-09-14 00:00:00

abstract：:A cDNA coding for the mouse hepatic triglyceride lipase has been isolated from a mouse liver cDNA library. The nucleotide sequence of the cDNA shows an open reading frame encoding a polypeptide of 510 amino acids that is 91.5% and 86% homologous to rat and human hepatic lipase, respectively. The most drastic protein s...

journal_title：FEBS letters

authors： Chang SF,Netter HJ,Will H

更新日期：1991-09-02 00:00:00

abstract：:LUSH is a soluble odorant-binding protein of the fruit fly Drosophila melanogaster. Mutants not expressing this protein have been reported to lack the avoidance behaviour, exhibited by wild type flies, to high concentrations of ethanol. Very recently, the three-dimensional structure of LUSH complexed with short-chain ...

journal_title：FEBS letters

authors： Zhou JJ,Zhang GA,Huang W,Birkett MA,Field LM,Pickett JA,Pelosi P

更新日期：2004-01-30 00:00:00

abstract：:Autophagy in the yeast is similar to that in mammalian cells. A mutant designated as apg1 (autophagy) defective in accumulation of autophagic bodies in the vacuoles was isolated by selection using a light microscope from a mutagenized proteinase-deficient strain. In the apg1 strain, which has normal vacuolar proteinas...

journal_title：FEBS letters

authors： Tsukada M,Ohsumi Y

更新日期：1993-10-25 00:00:00

abstract：:We have previously shown that unsaturated phosphatidylserines inhibit mitogen-induced T cell activation. We now report that the inhibitory action requires a protein present in bovine and human serum. Partial purification and phospholipase assay show that this protein has phospholipase A activity on phosphatidylserine ...

journal_title：FEBS letters

authors： Bellini F,Bruni A

更新日期：1993-01-18 00:00:00

abstract：:The relationship between active transport of lactose via the lactose permease and the protonmotive force has been determined in E. coli cells using either the respiratory chain inhibitor cyanide or protonophores to decrease the protonmotive force progressively. In contradiction with the prediction of the delocalized c...

journal_title：FEBS letters

authors： Ghazi A,Delamourd L,Shechter E

更新日期：1986-12-15 00:00:00