Absence of a unique relationship between active transport of lactose and protonmotive force in E. coli.


:The relationship between active transport of lactose via the lactose permease and the protonmotive force has been determined in E. coli cells using either the respiratory chain inhibitor cyanide or protonophores to decrease the protonmotive force progressively. In contradiction with the prediction of the delocalized chemiosmotic theory, two different relationships were obtained depending on the method used.




FEBS letters


Ghazi A,Delamourd L,Shechter E




Has Abstract


1986-12-15 00:00:00














  • Generation of Gla-domainless FVIIa by cathepsin G-mediated cleavage.

    abstract::Coagulation factor VII contains ten gamma-carboxyglutamic acid residues in the N-terminal region (Gla-domain) which are essential for the hemostatic function of FVII. The present study shows that granulocyte cathepsin G degrades the Gla-domain of FVIIa in vitro. Characterization of the truncated FVIIa by SDS-PAGE and ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nicolaisen EM,Petersen LC,Thim L,Jacobsen JK,Christensen M,Hedner U

    更新日期:1992-07-20 00:00:00

  • Crystal structure of intracellular family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium.

    abstract::The white-rot fungus Phanerochaete chrysosporium has two intracellular beta-glucosidases (BGL1A and BGL1B) belonging to glycoside hydrolase (GH) family 1. BGL1B effectively hydrolyzes cellobiose and cellobionolactone, but BGL1A does not. We have determined the crystal structure of BGL1A in substrate-free and gluconola...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nijikken Y,Tsukada T,Igarashi K,Samejima M,Wakagi T,Shoun H,Fushinobu S

    更新日期:2007-04-03 00:00:00

  • Drug-resistant malaria: molecular mechanisms and implications for public health.

    abstract::Resistance to antimalarial drugs has often threatened malaria elimination efforts and historically has led to the short-term resurgence of malaria incidences and deaths. With concentrated malaria eradication efforts currently underway, monitoring drug resistance in clinical settings complemented by in vitro drug susce...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Petersen I,Eastman R,Lanzer M

    更新日期:2011-06-06 00:00:00

  • Rescue of a neutralizing anti-viral antibody fragment from an intracellular polyclonal repertoire expressed in mammalian cells.

    abstract::The intracellular expression of recombinant antibodies in mammalian cells is an experimental strategy to inhibit in vivo the function of selected molecules. However, one limitation of this technology is represented by the unpredictable behaviour of antibodies, under conditions of intracellular expression. For this rea...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gargano N,Cattaneo A

    更新日期:1997-09-15 00:00:00

  • Fibronectin is a hypoxia-independent target of the tumor suppressor VHL.

    abstract::The von Hippel-Lindau (VHL) tumor suppressor gene regulates the extracellular matrix by controlling fibronectin deposition. To identify novel VHL target genes, we subjected mRNA from VHL-deficient RCC cells (786-0-pRC) and a transfectant re-expressing wildtype VHL (786-0-VHL) to differential expression profiling. Amon...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bluyssen HA,Lolkema MP,van Beest M,Boone M,Snijckers CM,Los M,Gebbink MF,Braam B,Holstege FC,Giles RH,Voest EE

    更新日期:2004-01-02 00:00:00

  • Peptidyl-prolyl cis-trans isomerase activity as studied by dynamic proton NMR spectroscopy.

    abstract::Recently the identity of the peptidyl-prolyl cis-trans isomerase (PPIase), which accelerates the cis/trans isomerization of prolyl peptide bonds and cyclophilin, the binding protein for the immunosuppressive drug Cyclosporin A (CsA), was discovered. The PPIase catalysis toward the substrate Suc-Ala-Phe-Pro-Phe-pNA has...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hübner D,Drakenberg T,Forsén S,Fischer G

    更新日期:1991-06-17 00:00:00

  • Inhibition of serum complement haemolytic activity by lipid vesicles containing phosphatidylserine.

    abstract::The effect of artificial model membranes on the complement system was investigated. Incubation of the model membranes with human serum resulted in consumption of complement haemolytic activity when phosphatidylserine-containing vesicles were used. The activation of the complement system appeared to proceed through the...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Comis A,Easterbrook-Smith SB

    更新日期:1986-03-03 00:00:00

  • Characterization of cytosolic malic enzyme in human tumor cells.

    abstract::Cytosolic NADP(+)-dependent malic enzyme (ME) from human tumor cells was characterized in detail and compared to ME from normal human tissues produced in recombinant E. coli. Kinetic properties, size as seen in SDS gels, and HPLC elution profiles of tryptic digests of human 'normal cell' ME and NADP(+)-ME from tumor c...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Loeber G,Dworkin MB,Infante A,Ahorn H

    更新日期:1994-05-16 00:00:00

  • Ceramide-1-phosphate promotes cell survival through activation of the phosphatidylinositol 3-kinase/protein kinase B pathway.

    abstract::In this report, we show for the first time that ceramide-1-phosphate (C1P) stimulates the phosphatidylinositol 3-kinase (PI3-K)/protein kinase B (PKB) pathway, which is a major mechanism whereby growth factors promote cell survival. Also, C1P induced IkappaB phosphorylation, and enhanced the DNA binding activity of th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gómez-Muñoz A,Kong JY,Parhar K,Wang SW,Gangoiti P,González M,Eivemark S,Salh B,Duronio V,Steinbrecher UP

    更新日期:2005-07-04 00:00:00

  • The amino acid sequence of the zinc-requiring beta-lactamase II from the bacterium Bacillus cereus 569.

    abstract::The amino acid sequence of the zinc-requiring beta-lactamase II from Bacillus cereus strain 569 has been determined. It consists of a single polypeptide chain of 227 residues. It is the only example so far fully characterized of a class B beta-lactamase, and is structurally and mechanistically distinct from both the w...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ambler RP,Daniel M,Fleming J,Hermoso JM,Pang C,Waley SG

    更新日期:1985-09-23 00:00:00

  • Tyrosine-rich acidic matrix protein (TRAMP) is a tyrosine-sulphated and widely distributed protein of the extracellular matrix.

    abstract::Tyrosine-rich acidic matrix protein (TRAMP; 22 kDa extracellular matrix protein; dermatopontin) is a protein that co-purifies with lysyl oxidase and with dermatan sulphate proteoglycans, with possible functions in cell-matrix interactions and matrix assembly. Using a rabbit polyclonal antiserum raised against porcine ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Forbes EG,Cronshaw AD,MacBeath JR,Hulmes DJ

    更新日期:1994-09-12 00:00:00

  • Co-transcription of Rhizobium meliloti lysyl-tRNA synthetase and glutamyl-tRNA synthetase genes.

    abstract::An open reading frame encoding a putative polypeptide very similar to several lysyl-tRNA synthetases was found 10 nucleotides downstream of Rhizobium meliloti gltX encoding glutamyl-tRNA synthetase. Expression of this gene complemented a mutation in lysS of Escherichia coli and led to the overexpression of a polypepti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pelchat M,Gagnon Y,Laberge S,Lapointe J

    更新日期:1999-04-16 00:00:00

  • A strategy for the generation of non-aggregating mutants of Anthozoa fluorescent proteins.

    abstract::Recently, we cloned several fluorescent proteins of different colors homologous to Aequorea victoria green fluorescent protein, which have great biotechnological potential as in vivo markers of gene expression. However, later investigations revealed severe drawbacks in the use of novel fluorescent proteins (FPs), in p...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yanushevich YG,Staroverov DB,Savitsky AP,Fradkov AF,Gurskaya NG,Bulina ME,Lukyanov KA,Lukyanov SA

    更新日期:2002-01-30 00:00:00

  • Proteolytic processing of the hepatocyte growth factor/scatter factor receptor by furin.

    abstract::The hepatocyte growth factor/scatter factor (HGF/SF) receptor consists of an alpha- and a beta-subunit, which are derived from a single-chain precursor by endoproteolytic processing. The precursor is not proteolytically processed in LoVo colon carcinoma cells. The uncleaved receptor immunopurified from the cells was c...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Komada M,Hatsuzawa K,Shibamoto S,Ito F,Nakayama K,Kitamura N

    更新日期:1993-08-09 00:00:00

  • Molecular dynamics simulation of four-alpha-helix bundles that bind the anesthetic halothane.

    abstract::The mutation of a single leucine residue (L38) to methionine (M) is known experimentally to significantly increase the affinity of the synthetic four-alpha-helix bundle (Aalpha(2))(2) for the anesthetic halothane. We present a molecular dynamics study of the mutant (Aalpha(2)-L38M)(2) peptide, which consists of a dime...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Davies LA,Zhong Q,Klein ML,Scharf D

    更新日期:2000-07-28 00:00:00

  • Identification and cloning of a novel cellular protein Naf1, Nef-associated factor 1, that increases cell surface CD4 expression.

    abstract::The nef gene of human and simian immunodeficiency virus is a key factor in acquired immunodeficiency syndrome pathogenesis and virus replication. Several Nef-induced phenomena, including the down-regulation of CD4 molecule, have been previously reported. In this study, we have identified and cloned a novel cellular pr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fukushi M,Dixon J,Kimura T,Tsurutani N,Dixon MJ,Yamamoto N

    更新日期:1999-01-08 00:00:00

  • Transcriptional regulation of murine NADP(+)-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase-synthetase.

    abstract::The cytosolic NADP(+)-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase is ubiquitously expressed in all mouse tissues and cell lines examined. Northern analyses of the RNA indicated that there is an extensive variation in the levels of mRNA in ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Peri KG,MacKenzie RE

    更新日期:1991-12-02 00:00:00

  • Purification and functional reconstitution of a truncated human Na(+)/glucose cotransporter (SGLT1) expressed in E. coli.

    abstract::A truncated human Na(+)/glucose cotransporter (C(5), residues 407-664) was expressed and purified from Escherichia coli using a GST fusion vector and glutathione affinity chromatography. The truncated transporter (C(5)) was cleaved from GST-C(5) by Factor Xa proteolysis and purified by gel filtration chromatography. U...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Panayotova-Heiermann M,Leung DW,Hirayama BA,Wright EM

    更新日期:1999-10-15 00:00:00

  • Expression of serotonin receptor mRNAs in blood vessels.

    abstract::Using RT-PCR we distinguished mRNAs for all known G-protein coupled serotonin receptors expressed in various rat and porcine blood vessels. Nearly all vessels expressed 5HT1D beta, 5-HT2A, 5-HT2B, 5-HT4, and 5-HT7 receptor mRNA to different extents. New splice variants of the porcine 5-HT4 receptor were observed. Simi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ullmer C,Schmuck K,Kalkman HO,Lübbert H

    更新日期:1995-08-21 00:00:00

  • Breakdown and synthesis of polyphosphoinositides in fMetLeuPhe-stimulated neutrophils.

    abstract::The interconversions of the inositol-containing lipids (PI, PI-P and PI-P2) and their products (DG, inositol phosphates and PA) in human and rabbit neutrophils stimulated with fMetLeuPhe and PMA have been examined. PMA causes only the phosphorylation of PI to PI-P whereas fMetLeuPhe causes phosphorylation of both PI a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cockcroft S,Barrowman MM,Gomperts BD

    更新日期:1985-02-25 00:00:00

  • Bidirectional communication between sensory neurons and osteoblasts in an in vitro coculture system.

    abstract::Recent studies have revealed that the sensory nervous system is involved in bone metabolism. However, the mechanism of communication between neurons and osteoblasts is yet to be elucidated. In this study, we investigated the signaling pathways between sensory neurons of the dorsal root ganglion (DRG) and the osteoblas...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Kodama D,Hirai T,Kondo H,Hamamura K,Togari A

    更新日期:2017-02-01 00:00:00

  • Beta-glucosidase activity towards a bile acid glucoside in human liver.

    abstract::Human liver contains hydrolytic activity toward 3 beta-glucosido-chenodeoxycholic acid. This beta-glucosidase activity, localized predominantly in the microsomal fraction, was optimally active in the presence of divalent metal ions close to pH 5.0 and was inhibited by EDTA. Kinetic parameters and other catalytic prope...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Matern H,Gartzen R,Matern S

    更新日期:1992-12-14 00:00:00

  • Purification of the skeletal muscle protein Endopin 1B and characterization of the genes encoding Endopin 1A and 1B isoforms.

    abstract::In the present work, a new endopin-like serpin designed mEndopin 1B was purified from bovine muscle. Biochemical characterizations (amino acid sequencing and Maldi-Tof mass spectrometry peptide mapping) demonstrated that the purified protein is different from the previously described Endopin 1, renamed mEndopin 1A. Th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Herrera-Mendez CH,Brémaud L,Coulis G,Pélissier P,Sentandreu MA,Aubry L,Delourme D,Chambon C,Maftah A,Leveziel H,Ouali A

    更新日期:2006-06-12 00:00:00

  • Primary and secondary structure of the 18 S ribosomal RNA of the insect species Tenebrio molitor.

    abstract::The sequence of the 18 S rRNA of Tenebrio molitor is reported. A detailed secondary structure model for eukaryotic small subunit rRNAs is proposed. The model comprises 48 universal helices that eukaryotic and prokaryotic small subunit rRNAs have in common, plus a number of helices in areas of variable secondary struct...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hendriks L,De Baere R,Van Broeckhoven C,De Wachter R

    更新日期:1988-05-09 00:00:00

  • Two-step mechanism of inhibition of cathepsin B by cystatin C due to displacement of the proteinase occluding loop.

    abstract::Stopped-flow kinetics showed that the inhibition of the lysosomal cysteine proteinase, cathepsin B, by its endogenous inhibitor, cystatin C, occurs by a two-step mechanism, in which an initial, weak interaction is followed by a conformational change. The initial interaction most likely involves binding of the N-termin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nycander M,Estrada S,Mort JS,Abrahamson M,Björk I

    更新日期:1998-01-23 00:00:00

  • Inositol trisphosphate-induced hyperpolarization in rat dorsal root ganglion neurons.

    abstract::Inositol 1,4,5-trisphosphate (1,4,5-InsP3) was perfused into rat dorsal root ganglion (DRG) neurons by whole-cell patch-clamp electrodes, while measuring the membrane potential. This operation evoked a transient (2-3 min) membrane hyperpolarization of about -15 mV (from -42 mV) followed by a depolarization. The membra...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wang Z,Ypey DL,Van Duijn B

    更新日期:1992-06-15 00:00:00

  • Identification of the cardiac ryanodine receptor channel in membrane blebs of sarcoplasmic reticulum.

    abstract::Blebs of the sarcoplasmic reticulum (SR) membrane of heart muscle cells were generated after saponin perforation of the plasma membrane followed by complete hypercontraction of the cell. Although characteristic proteins of the plasma membrane, namely the beta1-adrenoreceptor and Galphai, were stained by monoclonal ant...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Böhle T,Brandt MC,Henn N,Schmidt A,Bloch W,Beuckelmann DJ

    更新日期:2001-09-21 00:00:00

  • The interaction between the catalytic A subunit of calcineurin and its autoinhibitory domain, in the yeast two-hybrid system, is disrupted by cyclosporin A and FK506.

    abstract::The Ca(2+)-calmodulin dependent protein phosphatase, calcineurin, is thought to mediate the action of the two immunosuppressants, cyclosporin A (CsA) and FK506. Calcineurin from all species consists of a catalytic A subunit and a regulatory peptide B, which plays an essential role in catalysis. The enzymatic function ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chaudhuri B,Hämmerle M,Fürst P

    更新日期:1995-01-03 00:00:00

  • Photophysical properties of Prochlorococcus marinus SS120 divinyl chlorophylls and phycoerythrin in vitro and in vivo.

    abstract::Prochlorococcus marinus SS120 is an ecologically important and biochemically intriguing marine cyanobacterium. In addition to divinyl chlorophylls (DV-Chls) a and b it possesses a particular form of phycoerythrin (PE), but no other phycobilins and therefore no complete phycobilisomes. Here, a spectroscopic characteris...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Steglich C,Mullineaux CW,Teuchner K,Hess WR,Lokstein H

    更新日期:2003-10-09 00:00:00

  • Essential structure for full enterotoxigenic activity of heat-stable enterotoxin produced by enterotoxigenic Escherichia coli.

    abstract::Several analogues of heat-stable enterotoxins (STh and STp) produced by enterotoxigenic Escherichia coli were synthesized. Peptides (STh[6-18] and STp[5-17]) consisting of 13 amino acid residues from the Cys residue near the N-terminus to the Cys residue near the C-terminus and linked by three disulfide bonds had the ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yoshimura S,Ikemura H,Watanabe H,Aimoto S,Shimonishi Y,Hara S,Takeda T,Miwatani T,Takeda Y

    更新日期:1985-02-11 00:00:00