Skeletal muscle UCP2 and UCP3 gene expression in a rat cancer cachexia model.


:Rats bearing the Yoshida AH-130 ascites hepatoma showed an increased expression of both uncoupling protein-2 (UCP2) (194%) and UCP3 (189%) mRNA levels in skeletal muscle 7 days after tumour inoculation. Interestingly, an even greater increase was observed in mRNA for both UCP2 (278%) and UCP3 (797%) in the pair-fed animals, suggesting that the increase in gene expression was the result of the anorexia associated with tumour burden. The results constitute the first report of UCP2 and UCP3 gene expression during cancer cachexia and agree to their possible role in the increase of energy expenditure associated with tumour growth.




FEBS letters


Sanchís D,Busquets S,Alvarez B,Ricquier D,López-Soriano FJ,Argilés JM




Has Abstract


1998-10-09 00:00:00














  • Structure and thermal stability of the extracellular fragment of human transferrin receptor at extracellular and endosomal pH.

    abstract::Fourier transform infrared spectroscopy has been used to study the solution structure and thermal stability of the extracellular fragment of human transferrin receptor (tfRt) at extracellular and endosomal pH. At extracellular pH tfRt is composed of 56% alpha-helix, 19% beta-sheet and 14% turns. Upon acidification to ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hadden JM,Bloemendal M,Haris PI,van Stokkum IH,Chapman D,Srai SK

    更新日期:1994-08-22 00:00:00

  • The cytostatic activity of 5-(1-azidovinyl)-2'-deoxyuridine (AzVDU) against herpes simplex virus thymidine kinase gene-transfected FM3A cells is due to inhibition of thymidylate synthase and enhanced by UV light (lambda = 254 nm) exposure.

    abstract::5-(1-Azidovinyl)-2'-deoxyuridine (AzVDU) and a series of 5-[1-azido-2-halogenoethyl]-derivatives of beta-D-arabinofuranosyluracil (AU) proved markedly inhibitory to the replication of herpes simplex virus type 1 (HSV-1) and varicella zoster virus (VZV), but not thymidine kinase (TK)-deficient HSV-1 and VZV strains. No...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Balzarini J,Andrei G,Kumar R,Knaus EE,Wiebe LI,De Clercq E

    更新日期:1995-10-02 00:00:00

  • Between the ground- and M-state of bacteriorhodopsin the retinal transition dipole moment tilts out of the plane of the membrane by only 3 degrees.

    abstract::The orientation of the transition dipole moments in the ground state and the M-intermediate of bacteriorhodopsin were determined by time-resolved and steady-state polarized absorption spectroscopy on samples of oriented immobilized purple membranes. The angle between the transition dipole moment and the membrane norma...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Otto H,Heyn MP

    更新日期:1991-11-18 00:00:00

  • Trypanosomatid hydrogen peroxide [corrected] metabolism.

    abstract::The rate of whole cell H2O2 metabolism in several salivarian and stercorarian trypanosomes and Leishmania species was measured. These cells metabolized H2O2 at rates between 2.3 and 48.2 nmol/10(8) cells per min depending upon the species employed. H2O2 metabolism was largely insensitive to NaN3, implying that typical...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Penketh PG,Kennedy WP,Patton CL,Sartorelli AC

    更新日期:1987-09-14 00:00:00

  • Primary and post-irradiation inactivation of the sulfhydryl enzyme malate synthase: correlation of protective effects of additives.

    abstract::The presence of additives during X-irradiation of malate synthase led to radioprotective effects against primary and post-irradiation inactivation. Pronounced effects were provided by typical scavengers, sulfhydryl reagents and specific ligands (substrates, products, analogues). The results show that scavenging and sp...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Durchschlag H,Zipper P

    更新日期:1988-09-12 00:00:00

  • The interplay between structure and function in intrinsically unstructured proteins.

    abstract::Intrinsically unstructured proteins (IUPs) are common in various proteomes and occupy a unique structural and functional niche in which function is directly linked to structural disorder. The evidence that these proteins exist without a well-defined folded structure in vitro is compelling, and justifies considering th...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Tompa P

    更新日期:2005-06-13 00:00:00

  • Molecular action of the type 1 ribosome-inactivating protein saporin 5 on Vicia sativa ribosomes.

    abstract::The type 1 ribosome-inactivating protein (RIP) saporin 5 isolated from seeds of Saponaria officinalis L. strongly inhibited translation carried out by Vicia sativa L. purified ribosomes. The toxin multidepurinated V. sativa rRNA, which upon treatment with acid aniline releases several RNA fragments including an RNA fr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Iglesias R,Arias FJ,Rojo MA,Escarmis C,Ferreras JM,Girbés T

    更新日期:1993-07-05 00:00:00

  • Processing of complex between urokinase and its type-2 inhibitor on the cell surface. A possible regulatory mechanism of urokinase activity.

    abstract::Complexes between the urokinase-type plasminogen activator (uPA) and its type-2 inhibitor (PAI-2) are bound by a cell-surface receptor for uPA and rapidly cleaved into two fragments of 70 and 22 kDa. The 70-kDa fragment contains the active site of uPA and PAI-2, while the 22-kDa species was identified as the amino ter...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ragno P,Montuori N,Vassalli JD,Rossi G

    更新日期:1993-06-01 00:00:00

  • The RacGAP protein FilGAP is a negative regulator of chemokine-promoted lymphocyte migration.

    abstract::Rho family small GTPases regulate lymphocyte migration induced by chemokines. However, how lymphocyte migration is regulated by Rho GTPases remains to be elucidated. Here, we identified FilGAP, a Rac-specific GAP, as a negative regulator of lymphocyte polarization and migration. Depletion of FilGAP in mouse pro-B BAF ...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Iida T,Saito K,Katagiri K,Kinashi T,Ohta Y

    更新日期:2016-05-01 00:00:00

  • Selective inhibition of lignoceroyl-CoA synthetase by adenosine 5'-alkylphosphates.

    abstract::Structural analogs of adenosine 5'-acylphosphates, which are intermediates of the reaction catalyzed by acyl-CoA synthetases, were synthesized by condensing primary alcohols with AMP to examine the inhibitory effects on the lignoceroyl-CoA and palmitoyl-CoA synthetase activities. Hexadecyl, octadecyl, eicosyl, docosyl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Shiraishi T,Tezuka K,Uda Y

    更新日期:1994-10-03 00:00:00

  • Activation of Na,K-ATPase by an endogenous peptide, PEC-60.

    abstract::Several peptidic and non-peptidic factors can modulate Na,K-ATPase activity, among them mainly inhibitors of this enzyme, ouabain being the most effective. In a very few cases only, activation of Na,K-ATPase by endogenous factors has been recorded. We have investigated the effect on Na,K-ATPase of a novel regulatory p...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kairane C,Zilmer M,Mutt V,Sillard R

    更新日期:1994-05-23 00:00:00

  • Location of tolerated insertions/deletions in the structure of the maltose binding protein.

    abstract::In a previous study [(1987) J. Mol. Biol. 194, 663-673], we isolated ten insertion/deletion mutants (indels) of the maltose binding protein for which the maltose binding constant was only a little or not at all affected. In this paper, we have localized these mutations in the recently solved three-dimensional structur...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Betton JM,Martineau P,Saurin W,Hofnung M

    更新日期:1993-06-28 00:00:00

  • Proteins as strongly correlated protonic systems.

    abstract::Determination of the protonation state of enzymes is a challenging problem in computational biophysics largely due to the vast number of possible protonic configurations. The protonation state dynamics of respiratory complex I was investigated via Monte Carlo and asynchronous dynamics simulations and a novel eigenvect...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Couch V,Stuchebrukhov A

    更新日期:2012-03-09 00:00:00

  • Analysis of the activation state of alpha4beta1 integrin in human B cell lines derived from myeloma, leukemia or lymphoma.

    abstract::Myeloma cells specifically localize in the bone marrow and rarely circulate in blood. To study whether this immobilization could be partially explained by the presence of constitutively activated integrins, particularly alpha4beta1, we used the activation reporter HUTS-21 anti-beta1 mAb. These analyses showed that bet...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: García-Gila M,Cabañas C,García-Pardo A

    更新日期:1997-12-01 00:00:00

  • A common evolutionary origin of two elementary enzyme folds.

    abstract::The (beta alpha)(8)-barrel is the most frequent and most versatile fold among enzymes [Höcker et al., Curr. Opin. Biotechnol. 12 (2001) 376-381; Wierenga, FEBS Lett. 492 (2001) 193-198]. Structural and functional evidence suggests that (beta alpha)(8)-barrels evolved from an ancestral half-barrel, which consisted of f...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Höcker B,Schmidt S,Sterner R

    更新日期:2002-01-16 00:00:00

  • Catechin derivatives: specific inhibitor for caspases-3, 7 and 2, and the prevention of apoptosis at the cell and animal levels.

    abstract::Tea-catechin derivatives are shown to inhibit activities of caspases-3, 2 and 7 in vitro, and prevented experimental apoptosis at the cell and animal levels. Epigallo-catechin-gallate showed the strongest inhibition at 1 x 10(-7)M to these caspases, but cysteine cathepsins and caspase-8 were not inhibited. Caspase-3 i...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Katunuma N,Ohashi A,Sano E,Ishimaru N,Hayashi Y,Murata E

    更新日期:2006-02-06 00:00:00

  • Atomic force microscopy of DNA molecules.

    abstract::DNA-cytochrome c complexes adsorbed on carbon-coated mica surfaces were directly imaged by atomic force microscopy in air using commercially available cantilevers, with a routine resolution of 6 nm. Images of M13 phage DNA and M13-DNA polymerase complex are also shown. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yang J,Takeyasu K,Shao Z

    更新日期:1992-04-20 00:00:00

  • Overlapping CRE and E-box promoter elements can independently regulate COX-2 gene transcription in macrophages.

    abstract::Macrophage cyclooxygenase-2 (COX-2) transcription is mediated through the collaboration of different promoter elements. Here, the role of an overlapping cyclic AMP responsive element (CRE)/E-box was investigated. Nuclear proteins bound both the CRE and E-box, which synergized with other promoter elements to induce COX...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mestre JR,Rivadeneira DE,Mackrell PJ,Duff M,Stapleton PP,Mack-Strong V,Maddali S,Smyth GP,Tanabe T,Daly JM

    更新日期:2001-05-11 00:00:00

  • Studies on the interaction of the dye, stains-all, with individual calcium-binding domains of calmodulin.

    abstract::We show that the calcium-mimic dye, Stains-all, is a convenient probe to study the structural features of the individual calcium-binding sites of calmodulin (CaM) and related calcium-binding proteins (CaBP). These peptides bind the dye in their calcium-binding sites, and induce a circular dichroism (CD) band in the bo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sharma Y,Gopalakrishna A,Balasubramanian D,Fairwell T,Krishna G

    更新日期:1993-07-12 00:00:00

  • Bacterial expression of an active tyrosine kinase from a protein A/truncated c-src fusion protein.

    abstract::The carboxy-terminal half of the c-src protein fused to the protein A moiety was expressed in bacteria. The protein A/truncated c-src fusion protein, which does not have SH2 and SH3 domains, is found in the periplasmic space allowing for a simple one-step purification and demonstrated high efficiency in autophosphoryl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Saya H,Lee PS,Nishi T,Izawa I,Nakajima M,Gallick GE,Levin VA

    更新日期:1993-07-26 00:00:00

  • Foot-and-mouth disease virus leader proteinase: a papain-like enzyme requiring an acidic environment in the active site.

    abstract::Foot-and-mouth disease virus leader proteinase (L(pro)), a papain-like cysteine proteinase, has six acidic amino acids between 4 A and 11 A of the catalytic dyad of Cys51 and His148. In contrast, in papain and related enzymes, only one acidic residue lies within this distance. We have examined by site-directed mutagen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kronovetr J,Skern T

    更新日期:2002-09-25 00:00:00

  • Na,K-ATPase labelled with 5-iodoacetamidofluorescein: E2-E1 conformational transition induced by different nucleotides.

    abstract::A conformational transition between E2 and E1 forms of Na, K-ATPase induced by different nucleotides has been studied under steady state conditions using the enzyme labelled with 5-iodoacetamidofluorescein. In the presence of K+ the plot of fluorescence as a function of [ATP], [ADP] or [CTP] (in a range of 5 microM-12...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lopina OD,Pindel EV,Boldyrev AA

    更新日期:1990-06-18 00:00:00

  • Identification of an optimal Ncx binding sequence required for transcriptional activation.

    abstract::The Ncx gene encodes a homeobox containing transcription factor that belongs to the Hox11 gene family. We determined specific Ncx protein binding consensus DNA sequences. Optimal Ncx binding sequences were 5'-CGGTAATTGG-3' (TAAT core) and 5'-CGGTAAGTGG-3' (TAAG core), which coincided with the Hox11 binding sequence. B...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Shimizu H,Kang M,Iitsuka Y,Ichinose M,Tokuhisa T,Hatano M

    更新日期:2000-06-23 00:00:00

  • Using an in vivo phagemid system to identify non-compatible loxP sequences.

    abstract::The site-specific recombination system of bacteriophage P1 is composed of the Cre recombinase that recognizes a 34-bp loxP site. The Cre/loxP system has been extensively used to manipulate eukaryotic genomes for functional genomic investigations. The creation of additional heterologous loxP sequences potentially expan...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Siegel RW,Jain R,Bradbury A

    更新日期:2001-06-15 00:00:00

  • The 49-kDa subunit of NADH-ubiquinone oxidoreductase (Complex I) is involved in the binding of piericidin and rotenone, two quinone-related inhibitors.

    abstract::Piericidin is a potent inhibitor of the mitochondrial and bacterial type I NADH-ubiquinone oxidoreductases (Complex I) and is considered to bind at or close to the ubiquinone binding site(s) of the enzyme. Piericidin-resistant mutants of the bacterium Rhodobacter capsulatus have been isolated and the present work demo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Darrouzet E,Issartel JP,Lunardi J,Dupuis A

    更新日期:1998-07-10 00:00:00

  • Kinetic properties of cobalt--iron hybrid hemoglobins.

    abstract::The replacement of O2 with CO was studied on cobalt-iron hemoglobin hybrids. Both proto- and mesocobalt hemes were used for the reconstitution. In the oxy quaternary conformation no difference is observed between alpha- and beta-subunits when only proto hemes are present in the hybrid (k4 = 30 s-1, k'4/l'4 = 2.5). If ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Oertle M,Winterhalter KH,Di Iorio EE

    更新日期:1983-03-07 00:00:00

  • Inhibition of the mitochondrial cyclosporin A-sensitive permeability transition pore by the arginine reagent phenylglyoxal.

    abstract::The mitochondrial permeability transition pore, a cyclosporin A-sensitive channel, is controlled by the transmembrane electric potential difference across the inner membrane. Here, we show that treatment of rat liver mitochondria with the arginine reagent phenylglyoxal inhibits the permeability transition pore trigger...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Eriksson O,Fontaine E,Petronilli V,Bernardi P

    更新日期:1997-06-16 00:00:00

  • Phosphorylation of smooth muscle myosin light chain by five different kinases.

    abstract::Phosphorylation of the 20 kDa myosin light chain from smooth muscle by five different kinases was investigated. Three of the kinases (myosin light chain kinase, phosphorylase kinase, and cAMP-dependent protein kinase) phosphorylate serine residues, the fourth (casein-kinase-2) mainly threonine, and the fifth (glycogen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Singh TJ,Akatsuka A,Huang KP

    更新日期:1983-08-08 00:00:00

  • D-Aspartate stimulation of testosterone synthesis in rat Leydig cells.

    abstract::D-Aspartate increases human chorionic gonadotropin-induced testosterone production in purified rat Leydig cells. L-Aspartate, D-,L-glutamate or D-,L-asparagine could not substitute for D-aspartate and this effect was independent of glutamate receptor activation. Testosterone production was enhanced only in cells cultu...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nagata Y,Homma H,Lee JA,Imai K

    更新日期:1999-02-12 00:00:00

  • Molecular cloning of the canine angiotensin II receptor. An AT1-like receptor with reduced affinity for DuP753.

    abstract::Canine aortic strip studies revealed insensitivity of angiotensin II (AII)-induced aortic contraction to inhibition by the non-peptide antagonist DuP753 (pKB = 6.7 +/- 0.1). In order to determine the origin of this phenomenon we cloned the canine homologue of the AT1 AII receptor. Expression of this cDNA in COS-7 cell...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Burns L,Clark KL,Bradley J,Robertson MJ,Clark AJ

    更新日期:1994-04-25 00:00:00