Solution structure of an atypical WW domain in a novel beta-clam-like dimeric form.

abstract：:Golgin45 plays important roles in Golgi stack assembly and is known to bind both the Golgi stacking protein GRASP55 and Rab2 in the medial-Golgi cisternae. In this study, we sought to further characterize the cisternal adhesion complex using a proteomics approach. We report here that Acyl-CoA binding domain containing...

journal_title：FEBS letters

authors： Yue X,Bao M,Christiano R,Li S,Mei J,Zhu L,Mao F,Yue Q,Zhang P,Jing S,Rothman JE,Qian Y,Lee I

更新日期：2017-09-01 00:00:00

abstract：:Proteins involved in protein translocation across the membrane of the endoplasmic reticulum assemble into different oligomeric complexes depending on their state of function. To analyse such membrane protein complexes we fractionated proteins of mammalian rough microsomes and analysed them using blue native PAGE and i...

journal_title：FEBS letters

authors： Wang L,Dobberstein B

更新日期：1999-09-03 00:00:00

abstract：:Nuclear pore complexes (NPC) form nuclear pores that cross the nuclear envelope and allow molecules to transport between the nucleus and the cytoplasm. We solved the crystal structure of human Nup43 (hNUP43), an important component in the Nup107 subcomplex of NPC. hNup43 adopts a seven-bladed β-propeller fold. We conf...

journal_title：FEBS letters

authors： Xu C,Li Z,He H,Wernimont A,Li Y,Loppnau P,Min J

更新日期：2015-10-24 00:00:00

abstract：:Streptomyces lividans 1326-9, a bldA+ strain, and its bldA39 mutant derivative J1725 were transformed with a cosmid containing the pur cluster, which determines the puromycin biosynthetic pathway from Streptomyces alboniger. bldA+ transformants produced puromycin in typical amounts, whereas bldA39 transformants did so...

journal_title：FEBS letters

authors： Tercero JA,Espinosa JC,Jiménez A

更新日期：1998-01-16 00:00:00

abstract：:The gap junctional intercellular communication mediated by Cx43 plays indispensable roles in both germ line development and postnatal folliculogenesis. In this study, we focused on the effect of follicle-stimulating hormone (FSH) on the Cx43 protein in rat primary granulosa cells and found that FSH stimulation elevate...

journal_title：FEBS letters

authors： Yogo K,Ogawa T,Akiyama M,Ishida N,Takeya T

更新日期：2002-11-06 00:00:00

abstract：:Phosphorylation of histone variant H2AX at serine 139, named gammaH2AX, has been widely used as a sensitive marker for DNA double-strand breaks (DSBs). gammaH2AX is required for the accumulation of many DNA damage response (DDR) proteins at DSBs. Thus it is believed to be the principal signaling protein involved in DD...

journal_title：FEBS letters

authors： Yuan J,Adamski R,Chen J

更新日期：2010-09-10 00:00:00

abstract：:G-quadruplex structures in the 5' UTR of mRNAs have been shown to suppress translation. However, all experiments published so far made use of artificial reporter systems. Here, we investigate for the first time the biological effects of the genomic disruption of a G-quadruplex by CRISPR/Cas9. In melanoma cells lacking...

journal_title：FEBS letters

authors： Serikawa T,Eberle J,Kurreck J

更新日期：2017-11-01 00:00:00

abstract：:Glycogen phosphorylase b at concentrations close to those found in skeletal muscle interacts with sarcoplasmic reticulum membranes, but not with liposomes made of lipids extracted from these membranes, and is inhibited upon binding to the membrane. The interaction of glycogen phosphorylase with the sarcoplasmic reticu...

journal_title：FEBS letters

authors： Cuenda A,Centeno F,Gutierrez-Merino C

更新日期：1991-06-03 00:00:00

abstract：:Binding of vitronectin (VN) to Neisseria gonorrhoeae expressing the heparan sulfate proteoglycan (HSPG) specific Opa50 protein was recently shown to trigger bacterial internalization into distinct epithelial cell lines. We have investigated the role of VN-binding integrin receptors and protein kinase C (PKC) in VN-tri...

journal_title：FEBS letters

authors： Dehio M,Gómez-Duarte OG,Dehio C,Meyer TF

更新日期：1998-03-06 00:00:00

abstract：:The amino acid sequences of two ribonucleases from a callus cell culture of Panax ginseng were determined. The two sequences differ at 26% of the amino acid positions. Homology was found with a large family of intracellular pathogenesis-related proteins, food allergens and tree pollen allergens from both dicotyledonou...

journal_title：FEBS letters

authors： Moiseyev GP,Fedoreyeva LI,Zhuravlev YN,Yasnetskaya E,Jekel PA,Beintema JJ

更新日期：1997-04-28 00:00:00

abstract：:Lactate dehydrogenase (LDH) and yeast alcohol dehydrogenase ( YADH ) are inactivated when attacked by hydroxy free radicals (OH). Organic molecules with a high rate constant of reaction with OH such as ascorbate or urate can compete with the enzymes for these strongly oxidising radicals. However, although 10(-3)M asco...

journal_title：FEBS letters

authors： Kittridge KJ,Willson RL

更新日期：1984-05-07 00:00:00

abstract：:The effect of galanin (GAL) on basal and phorbol-12,13-dibutyrate (PDBu) induced protein phosphorylation in rat ventral hippocampal miniprisms was investigated. GAL (0.5, 1 and 2 microM) inhibited PDBu stimulation in a concentration-dependent manner without altering basal protein phosphorylation. This inhibitory effec...

journal_title：FEBS letters

authors： La Porta C,Bianchi R,Sozzani S,Bartfai T,Consolo S

更新日期：1992-03-23 00:00:00

abstract：:Antimycin, 2-nonyl-4-hydroxyquinoline N-oxide and funiculosin induce O.2(-) generation by submitochondrial particles oxidizing succinate, whereas KCN, mucidin, myxothiazol or 2,3-dimercaptopropanol inhibit O.2(-) generation. Thenoyltrifluoroacetone does not induce superoxide production by itself but slightly stimulate...

journal_title：FEBS letters

authors： Ksenzenko M,Konstantinov AA,Khomutov GB,Tikhonov AN,Ruuge EK

更新日期：1983-05-02 00:00:00

abstract：:Photosystem I (PSI) complex of Anabaena variabilis ATCC 29413 consists of at least 11 subunits, 9 of which are resolved by high resolution gel electrophoresis. N-terminal amino acid sequences of the four subunits with molecular masses of 6.8, 5.2, 4.8 and 3.5 kDa were determined. Based on the sequence homology, the 3....

journal_title：FEBS letters

authors： Ikeuchi M,Nyhus KJ,Inoue Y,Pakrasi HB

更新日期：1991-08-05 00:00:00

abstract：:Hepatitis B virus X protein is a major factor in the HBV-induced disease developments. Stromal cell-derived factor-1 is a small cytokine that is strongly chemotactic for lymphocytes. We explored the role of HBx on recruitment of HBV-induced virus-nonspecific immune cells into liver. Immune cell recruitment and SDF-1 e...

journal_title：FEBS letters

authors： Cho HK,Kim SY,Seong JK,Cheong J

更新日期：2014-03-03 00:00:00

abstract：:The covalent conjugation of oligonucleotides to antibody Fab' fragments was optimized by using oligonucleotides modified with a hexaethylene linker arm bearing three amino groups. One oligonucleotide was coupled to antibody of one specificity and a complementary oligonucleotide to antibody of a second specificity. The...

journal_title：FEBS letters

authors： Chaudri ZN,Bartlet-Jones M,Panayotou G,Klonisch T,Roitt IM,Lund T,Delves PJ

更新日期：1999-04-30 00:00:00

abstract：:Lung surfactant secretion is a highly regulated process. Our previous studies have shown that VAMP-2 is essential for surfactant secretion. In the present study we investigated the role of miR-206 in surfactant secretion through VAMP-2. VAMP-2 was confirmed to be a target of miR-206 by 3'-untranslational region (3'-UT...

journal_title：FEBS letters

authors： Zhang H,Guo Y,Mishra A,Gou D,Chintagari NR,Liu L

更新日期：2015-01-02 00:00:00

abstract：:Criteria for the design of peptide vaccines to prevent AIDS are presented. The best vaccine candidates contain both B and T lymphocyte-defined epitopes in regions conserved in sequence between viral isolates. We propose that attention should focus on proteins specified by the gag and, possibly, pol genes in addition t...

journal_title：FEBS letters

authors： Sternberg MJ,Barton GJ,Zvelebil MJ,Cookson J,Coates AR

更新日期：1987-06-29 00:00:00

abstract：:Trypsin and chymotrypsin have specificity pockets of essentially the same geometry, yet trypsin is specific for basic while chymotrypsin for bulky hydrophobic residues at the P1 site of the substrate. A model by Steitz, Henderson and Blow suggested the presence of a negative charge at site 189 as the major specificity...

journal_title：FEBS letters

authors： Venekei I,Szilágyi L,Gráf L,Rutter WJ

更新日期：1996-01-29 00:00:00

abstract：:Mature dermal glands of Xenopus laevis contain storage granules with a characteristic ellipsoid shape. A few major proteins are present in these granules, including two with the same amino-terminal sequence and apparent molecular masses of 26 and 28 kDa. Using antibodies against these proteins, positive clones were is...

journal_title：FEBS letters

authors： Berger H,Kreil G

更新日期：1989-06-05 00:00:00

abstract：:miRNAs play a number of roles in bone, including mediating the pathological effects of inflammation. Here, we found that miR-33a-5p expression was significantly increased after TNF-α treatment during BMP-2-induced osteogenic differentiation of hBMSCs. Luciferase reporter assays and western blotting demonstrated that s...

journal_title：FEBS letters

authors： Mi W,Shi Q,Chen X,Wu T,Huang H

更新日期：2016-02-01 00:00:00

abstract：:hsp25 is a small, growth-related, mammalian stress protein which is highly accumulated in the stationary phase of Ehrlich ascites tumor in vivo. Ehrlich ascites cells cultivated in vitro under conditions of continuous exponential growth express hsp25 only at a low level. These cells were stably transfected with an euk...

journal_title：FEBS letters

authors： Knauf U,Bielka H,Gaestel M

更新日期：1992-09-14 00:00:00

abstract：:Three regions of the alpha chain of Torpedo californica acetylcholine receptor (AChR), corresponding to residues alpha 262-276, alpha 388, 408 and alpha 427-437 were synthesized, purified and characterized. The first two peptides have been proposed to occupy inter-transmembrane regions while the third represented the ...

journal_title：FEBS letters

authors： Atassi MZ,Manshouri T,Yokoi T

更新日期：1988-02-15 00:00:00

abstract：:LncRNAs have a critical role in the regulation of cellular processes such as cancer progression and metastasis. In the present study, we confirmed that TUG1 was overexpressed in bladder cancer tissues and established cell lines. Knockdown of TUG1 inhibited bladder cancer cell metastasis both in vitro and in vivo. Furt...

journal_title：FEBS letters

authors： Tan J,Qiu K,Li M,Liang Y

更新日期：2015-10-07 00:00:00

abstract：:Hepatitis C Virus core protein (HCVc) plays important roles in the development of intrahepatic cholangiocarcinoma (ICC). MicroRNAs (miRNAs) contribute to tumor progression by interacting with downstream target genes. However, the regulation and role of miRNAs in HCV-related intrahepatic cholangiocarcinoma (HCV-ICC) is...

journal_title：FEBS letters

authors： Zeng B,Li Z,Chen R,Guo N,Zhou J,Zhou Q,Lin Q,Cheng D,Liao Q,Zheng L,Gong Y

更新日期：2012-09-21 00:00:00

abstract：:p38, which has been suggested to be a scaffold protein for the assembly of a macromolecular tRNA synthetase complex, contains a leucine zipper-like motif. To understand the importance of the leucine zipper-like motif of p38 (p38LZ) in macromolecular assembly, the p38LZ solution structure was investigated by circular d...

journal_title：FEBS letters

authors： Ahn HC,Kim S,Lee BJ

更新日期：2003-05-08 00:00:00

abstract：:The phosphoglucomutases (PGM) Pgm1, Pgm2, and Pgm3 of the yeast Saccharomyces cerevisiae were tested for their ability to interconvert ribose-1-phosphate and ribose-5-phosphate. The purified proteins were studied in vitro with regard to their kinetic properties on glucose-1-phosphate and ribose-1-phosphate. All tested...

journal_title：FEBS letters

authors： Walther T,Baylac A,Alkim C,Vax A,Cordier H,François JM

更新日期：2012-11-30 00:00:00

abstract：:A method was established for measuring molecule numbers of three different myosin heavy chain (MHC) mRNA isoforms in total RNA preparations. The quantification was based on a combination of primer-directed reverse transcriptase and polymerase chain reactions with 5'-digoxigenin-labeled oligonucleotides, using external...

journal_title：FEBS letters

authors： Peuker H,Pette D

更新日期：1993-03-08 00:00:00

abstract：:The complete amino acid sequence of porcine muscarinic acetylcholine receptor III has been deduced by cloning and sequencing the genomic DNA. The antagonist binding properties of muscarinic acetylcholine receptor III expressed from the cloned DNA in Xenopus oocytes correspond most closely to those of the pharmacologic...

journal_title：FEBS letters

authors： Akiba I,Kubo T,Maeda A,Bujo H,Nakai J,Mishina M,Numa S

更新日期：1988-08-01 00:00:00

abstract：:The remarkable stability of c-myc during oogenesis contrasts with its degradation during the early developmental period in Xenopus laevis. Three evolutionary conserved motifs found in the 3'-untranslated region of Xenopus c-myc RNAs have been analyzed for a possible role in c-myc RNA degradation. No specific degradati...

journal_title：FEBS letters

authors： Tchang F,Vriz S,Méchali M

更新日期：1991-10-21 00:00:00