Chaos in DNA partition during the last mitoses of the proliferative life-span of human fibroblasts.

abstract：:Mutations in the nuclear matrix protein Matrin 3 (MATR3) have been identified in amyotrophic lateral sclerosis and myopathy. To investigate the mechanisms underlying MATR3 mutations in neuromuscular diseases and efficiently screen for modifiers of MATR3 toxicity, we generated transgenic MATR3 flies. Our findings indic...

journal_title：FEBS letters

authors： Zhao M,Kao CS,Arndt C,Tran DD,Cho WI,Maksimovic K,Chen XXL,Khan M,Zhu H,Qiao J,Peng K,Hong J,Xu J,Kim D,Kim JR,Lee J,van Bruggen R,Yoon WH,Park J

更新日期：2020-09-01 00:00:00

abstract：:To investigate the role of lipopolysaccharide (LPS) in hepatocyte activation, we examined the expression of Toll-like receptor 4 (TLR4), the putative receptor for LPS in human hepatocytes. TLR4 mRNA and protein expression was confirmed in human hepatocytes. Stimulation of human hepatocytes with LPS results in rapid de...

journal_title：FEBS letters

authors： Migita K,Abiru S,Nakamura M,Komori A,Yoshida Y,Yokoyama T,Daikoku M,Ueki T,Takii Y,Yano K,Yastuhashi H,Eguchi K,Ishibashi H

更新日期：2004-07-02 00:00:00

abstract：:We report a new member of the von Willebrand factor A-domain protein superfamily, WARP (for von Willebrand factor A-domain-related protein). The full-length mouse WARP cDNA is 2.3 kb in size and predicts a protein of 415 amino acids which contains a signal sequence, a VA-like domain, two fibronectin type III-like repe...

journal_title：FEBS letters

authors： Fitzgerald J,Tay Ting S,Bateman JF

更新日期：2002-04-24 00:00:00

abstract：:The accumulation of protoporphyrin IX (Proto IX) in light-sensitive mutants of Escherichia coli was detected by spectrofluorimetry. Fluorescence emission and excitation spectra were recorded from extracts of bacterial cells. Proto IX clearly accumulated in cells with mutations in the visA (hemH) gene but not in the wi...

journal_title：FEBS letters

authors： Miyamoto K,Nishimura K,Masuda T,Tsuji H,Inokuchi H

更新日期：1992-10-05 00:00:00

abstract：:The degree of conservation of HeLa interphase chromatin nonhistone antigens among the nonhistones of isolated metaphase chromosomes was determined with immunological procedures. Proteins were separated on SDS-polyacrylamide gels and electrophoretically transferred to diazophenylthioether (DPT)-paper, which was then ov...

journal_title：FEBS letters

authors： Adolph KW

更新日期：1984-01-09 00:00:00

abstract：:Fibroblasts were exposed to various concentrations of hydrogen peroxide and the removal of oxidized proteins was followed by determining protein-bound carbonyls. Fibroblasts are able to increase the turnover of metabolically radiolabeled proteins after treatment with hydrogen peroxide. It was demonstrated for the firs...

journal_title：FEBS letters

authors： Sitte N,Merker K,Grune T

更新日期：1998-12-04 00:00:00

abstract：:There is considerable epidemiological evidence that tea drinking lowers the risk of heart disease. However, the mechanism by which tea can be protective is unknown. Hamsters were fed a normal or high cholesterol diet for 2 weeks and drank green or black tea ad libitum. The plasma lipid profile was significantly improv...

journal_title：FEBS letters

authors： Vinson JA,Dabbagh YA

更新日期：1998-08-14 00:00:00

abstract：:High-molecular-weight [14C]hyaluronate was incubated with cultured fibroblasts from human uterine cervix and skin, and then the depolymerization of the hyaluronate was investigated. [14C]Hyaluronate in the medium of skin fibroblasts was depolymerized into a constant molecular weight (M(r) about 40,000), whereas that o...

journal_title：FEBS letters

authors： Tanaka K,Nakamura T,Ikeya H,Higuchi T,Tanaka A,Morikawa A,Saito Y,Takagaki K,Endo M

更新日期：1994-06-20 00:00:00

abstract：:The cDNA coding for mature human alpha 1-proteinase inhibitor (alpha 1-PI) has been inserted into a variety of yeast expression vectors. Yeast cells transformed with these plasmids were then assayed for the production of mature, unglycosylated alpha 1-PI. The production level is optimal when the recombinant plasmid ca...

journal_title：FEBS letters

authors： Hoylaerts M,Weyens A,Bollen A,Harford N,Cabezón T

更新日期：1986-08-11 00:00:00

abstract：:The spike (S) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is an important viral structural protein. Based on bioinformatics analysis, 10 antigenic peptides derived from the S protein sequence were selected and synthesized. The antigenicity and immunoreactivity of all the peptides were tested in...

journal_title：FEBS letters

authors： Lu W,Wu XD,Shi MD,Yang RF,He YY,Bian C,Shi TL,Yang S,Zhu XL,Jiang WH,Li YX,Yan LC,Ji YY,Lin Y,Lin GM,Tian L,Wang J,Wang HX,Xie YH,Pei G,Wu JR,Sun B

更新日期：2005-04-11 00:00:00

abstract：:Uptake of cholesterol by isolated rat hepatocytes in a serum-free medium was remarkably enhanced by dispersion with several kinds of phosphatidylcholine. Of the various phosphatidylcholines tested, dilinoylphosphatidylcholine had the strongest accelerating effect, while dipalmitoylphosphatidylcholine was the weakest. ...

journal_title：FEBS letters

authors： Nagamura Y,Sasaki K,Ito M,Saito K,Ogitsu N,Ishiguro I

更新日期：1986-07-14 00:00:00

abstract：:In human brain the Abeta peptide is produced mainly by neurons and the overexpression of amyloid precursor protein (APP) that involves an increase in Abeta secretion, has been observed in some areas of the Alzheimer's disease patients brain. We have generated two stably transfected human neuroblastoma lines which over...

journal_title：FEBS letters

authors： Recuero M,Serrano E,Bullido MJ,Valdivieso F

更新日期：2004-07-16 00:00:00

abstract：:A selective PKC inhibitor, UCN-01, was shown to exhibit anti-tumor activity in vitro and in vivo. We investigated UCN-01 with respect to isozyme-specific PKC inhibition using purified recombinant or rabbit brain PKC isozymes, cPKC alpha, beta and gamma, nPKC delta, epsilon and eta, and a PKC zeta. Of the PKC isozymes ...

journal_title：FEBS letters

authors： Mizuno K,Noda K,Ueda Y,Hanaki H,Saido TC,Ikuta T,Kuroki T,Tamaoki T,Hirai S,Osada S

更新日期：1995-02-13 00:00:00

abstract：:The internalisation of metabotropic glutamate receptor (mGluR1alpha) and its splice variant (mGluR1beta), in response to agonist and phorbol esters (PMA), has been studied. Both mGluR1alpha and mGluR1beta exhibit a similar rate of internalisation following PMA treatment, with a shift in their distribution from plasma ...

journal_title：FEBS letters

authors： Ciruela F,McIlhinney RA

更新日期：1997-11-24 00:00:00

abstract：:MDNCF is a human monocyte-derived, 72-residue chemotactic peptide, which has sequence similarity with members of a family of pro-inflammatory cytokines. The peptide was synthesized by the solid-phase method, and is identical to the natural peptide in amino acid composition, sequence and chemotactic potency. MDNCF form...

journal_title：FEBS letters

authors： Tanaka S,Robinson EA,Yoshimura T,Matsushima K,Leonard EJ,Appella E

更新日期：1988-08-29 00:00:00

abstract：:We describe here a 70 kDa transcription factor A1F-B, which preferentially binds to an element encompassing a CCAAT motif on the rat aldolase B promoter. Comparison of binding specificities, relative molecular masses, and subunit compositions with those of other known CCAAT-binding factors indicated that A1F-B is a no...

journal_title：FEBS letters

authors： Tsutsumi K,Ito K,Yabuki T,Ishikawa K

更新日期：1993-04-19 00:00:00

abstract：:Genetic factors seem to play a significant role in susceptibility to systemic lupus erythematosus (SLE). We previously described the amino acid polymorphism (Val14Met) within the IFN-gamma receptor 1 (IFN-gammaRI), and that the frequency of the Metl4 allele in SLE patients was significantly higher than that of the hea...

journal_title：FEBS letters

authors： Nakashima H,Inoue H,Akahoshi M,Tanaka Y,Yamaoka K,Ogami E,Nagano S,Arinobu Y,Niiro H,Otsuka T,Niho Y

更新日期：1999-06-18 00:00:00

abstract：:The CA microsatellite repeat marker, D7S522, is located at the center of a approximately 1000 kb smallest common deleted region that is lost in many forms of human cancer. It has been proposed that a putative tumor suppressor gene lies in close proximity to D7S522, within this smallest common deleted region. However, ...

journal_title：FEBS letters

authors： Engelman JA,Zhang XL,Lisanti MP

更新日期：1999-04-09 00:00:00

abstract：:Among DNA repair pathways, nucleotide excision repair (NER) is able to recognize and process a wide variety of DNA lesions. The NER mechanism can be summarized in two stages: incision/excision of the lesion and DNA repair synthesis. Here, we have assessed the repair synthesis activity of protein extracts from differen...

journal_title：FEBS letters

authors： Coudoré F,Calsou P,Salles B

更新日期：1997-09-15 00:00:00

abstract：:Prefibrillar cytotoxicity was suggested as a common amyloid characteristic. We showed two types of albebetin prefibrillar oligomers are formed during incubation at pH 7.3. Initial round-shaped oligomers consist of 10-15 molecules determined by atomic force microscopy, do not bind thioflavin-T and do not affect viabili...

journal_title：FEBS letters

authors： Zamotin V,Gharibyan A,Gibanova NV,Lavrikova MA,Dolgikh DA,Kirpichnikov MP,Kostanyan IA,Morozova-Roche LA

更新日期：2006-05-01 00:00:00

abstract：:The role of the RNA-binding protein human antigen R (HuR) in hepatocarcinogenesis is still elusive. By employing short hairpin (sh)RNA-dependent knockdown approach, we demonstrate that lymphotoxin α (LTα) is a target of posttranscriptional gene regulation by HuR in hepatocellular carcinoma (HepG2) cells. Consequently,...

journal_title：FEBS letters

authors： Steinmeyer N,Doller A,Biyanee A,Brauss T,Schmid T,Pfeilschifter J,Eberhardt W

更新日期：2015-07-08 00:00:00

abstract：:DNA complementary to the rat hypothalamic mRNA coding for the corticotropin-releasing factor precursor (prepro-CRF) has been cloned by screening a cDNA library with a human genomic DNA probe. Nucleotide sequence analysis of the cloned cDNA has revealed that rat prepro-CRF consists of 187 amino acid residues including ...

journal_title：FEBS letters

authors： Jingami H,Mizuno N,Takahashi H,Shibahara S,Furutani Y,Imura H,Numa S

更新日期：1985-10-21 00:00:00

abstract：:SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)1H-imi dazole) is widely used as a specific inhibitor of p38 mitogen-activated protein kinase (MAPK). Here, we report that SB203580 activates the serine/threonine kinase Raf-1 in quiescent smooth muscle cells in a dose-dependent fashion. The concentr...

journal_title：FEBS letters

authors： Kalmes A,Deou J,Clowes AW,Daum G

更新日期：1999-02-05 00:00:00

abstract：:In the present study we have examined the levels and phosphorylation state of the insulin receptor and insulin receptor substrate 1 (IRS-1) as well as the association between IRS-1 and phosphatidylinositol 3-kinase (PI 3-kinase) in the liver and muscle of rats treated with glucagon. There was a decrease in the insulin...

journal_title：FEBS letters

authors： Saad MJ,Hartmann LG,de Carvalho DS,Galoro CA,Brenelli SL,Carvalho CR

更新日期：1995-08-14 00:00:00

abstract：:The purinergic P2X(7) receptor is an ATP-receptor channel predominantly expressed in immune cells. P2X(7) has been cloned from human, rat and mouse. Here we report cloning of the Xenopus laevis P2X(7) receptor (xP2X(7)). xP2X(7) is only about 50% identical to the mammalian homologues, shows a broad tissue expression p...

journal_title：FEBS letters

authors： Paukert M,Hidayat S,Gründer S

更新日期：2002-02-27 00:00:00

abstract：:Both cisplatin and the estrogen receptor (ER) are known to bend DNA. The influence of the bending of sequences by the d(GpG)cisPt adduct binding of ER to estrogen response element (ERE)-like sequences was examined. Three ERE-like oligonucleotides with different affinities for ER and which include a GG in the linker se...

journal_title：FEBS letters

authors： Massaad-Massade L,Massaad C,Legendre F,Bas V,Chottard J,Beaune P,Barouki R

更新日期：2000-01-21 00:00:00

abstract：:The modification of homeodomain-interacting protein kinase 2 (HIPK2) by small ubiquitin-like modifier 1 (SUMO-1) plays an important role in its targeting into the promyelocytic leukemia body, as well as in its differential interaction with binding partner, but the desumoylation of HIPK2 by SUMO-specific proteases is l...

journal_title：FEBS letters

authors： Kim YH,Sung KS,Lee SJ,Kim YO,Choi CY,Kim Y

更新日期：2005-11-07 00:00:00

abstract：:We have developed a high-throughput sequencing approach that enables us to determine terminal telomere sequences from tens of thousands of individual Schizosaccharomyces pombe telomeres. This method provides unprecedented coverage of telomeric sequence complexity in fission yeast. S. pombe telomeres are composed of mo...

journal_title：FEBS letters

authors： Bennett HW,Liu N,Hu Y,King MC

更新日期：2016-12-01 00:00:00

abstract：:Communication between endothelial and smooth muscle cells (SMCs) contributes to atherosclerosis induced by atherogenic factors, such as oxide LDL. Asymmetric dimethylarginine (ADMA), a newly found cardiovascular risk factor, accumulates in the culture medium of oxide LDL (oxLDL)-treated endothelial cells and positivel...

journal_title：FEBS letters

authors： Sun L,Zhang T,Yu X,Xin W,Lan X,Zhang D,Huang C,Du G

更新日期：2011-09-02 00:00:00

abstract：:The site-specific recombination system of bacteriophage P1 is composed of the Cre recombinase that recognizes a 34-bp loxP site. The Cre/loxP system has been extensively used to manipulate eukaryotic genomes for functional genomic investigations. The creation of additional heterologous loxP sequences potentially expan...

journal_title：FEBS letters

authors： Siegel RW,Jain R,Bradbury A

更新日期：2001-06-15 00:00:00