Synthesis and biological characterization of monocyte-derived neutrophil chemotactic factor.

abstract：:A potent inhibitor of a dual-specificity protein phosphatase, VHR (vaccinia H1 related), was isolated during a screening of microbial metabolites. This inhibitor was identified as 4-isoavenaciolide (4-iA), and was determined to irreversibly inhibit VHR phosphatase activity with a 50% inhibitory concentration of 1.2 mi...

journal_title：FEBS letters

authors： Ueda K,Usui T,Nakayama H,Ueki M,Takio K,Ubukata M,Osada H

更新日期：2002-08-14 00:00:00

abstract：:Creatine kinases (CK) play a prominent role in cell energy distribution through an energy shuttle between mitochondria and other organelles. Human brain CK was cloned and overexpressed in COS-7 cells. We then deleted His-65 and/or Pro-66 situated near the center of a flexible loop as shown by X-ray crystallography on ...

journal_title：FEBS letters

authors： Mourad-Terzian T,Steghens JP,Min KL,Collombel C,Bozon D

更新日期：2000-06-09 00:00:00

abstract：:The orientation of the transition dipole moments in the ground state and the M-intermediate of bacteriorhodopsin were determined by time-resolved and steady-state polarized absorption spectroscopy on samples of oriented immobilized purple membranes. The angle between the transition dipole moment and the membrane norma...

journal_title：FEBS letters

authors： Otto H,Heyn MP

更新日期：1991-11-18 00:00:00

abstract：:We used site-directed mutagenesis to modify the Bacillus thuringiensis cry3A gene in amino acid residues 350-354. Two mutant toxins, A1 (R(345)A,Y(350)F,Y(351)F) and A2 (R(345)A,DeltaY(350), DeltaY(351)), showed significantly improved toxicity against Tenebrio molitor (yellow mealworm). The mutant toxin A1 was also mo...

journal_title：FEBS letters

authors： Wu SJ,Koller CN,Miller DL,Bauer LS,Dean DH

更新日期：2000-05-12 00:00:00

abstract：:Escherichia coli phosphofructokinase-2 (Pfk-2) is a homodimer whose subunits consist of a large domain and an additional beta-sheet that provides the interfacial contacts between the subunits, creating a beta-barrel flattened-like structure with the adjacent subunit's beta-sheet. To determine how the structural organi...

journal_title：FEBS letters

authors： Baez M,Babul J

更新日期：2009-06-18 00:00:00

abstract：:An experimental procedure is described for the reproducible production of hybridoma cells using the electrofusion technique. High yields can be obtained when fusion is performed in isotonic inositol solutions containing Ca2+ and Mg2+ in a ratio of 1:5 in the millimolar range. The hybridoma cells are transferred 10 min...

journal_title：FEBS letters

authors： Vienken J,Zimmermann U

更新日期：1985-03-25 00:00:00

abstract：:In the mouse macrophage-like cell line RAW 264, vacuolar-type (H(+))-ATPase (V-ATPase) inhibitors, bafilomycin A(1) and concanamycin A, increased the level of cyclooxygenase (COX)-2 protein and its mRNA. The V-ATPase inhibitor-induced expression of COX-2 was suppressed by inhibitors of c-jun N-terminal kinase (JNK) an...

journal_title：FEBS letters

authors： Kamachi F,Yanai M,Ban HS,Ishihara K,Hong J,Ohuchi K,Hirasawa N

更新日期：2007-10-02 00:00:00

abstract：:Bispecific miniantibodies were constructed by genetically fusing the C(H)1 domain of an IgG1 to the C-terminus of a single-chain Fv fragment (scFv-425), specific for the EGF receptor, and fusing the C(L) domain of a kappa light chain to the C-terminus of a scFv specific for CD2 (scFv-M1). An efficient dicistronic gene...

journal_title：FEBS letters

authors： Müller KM,Arndt KM,Strittmatter W,Plückthun A

更新日期：1998-01-30 00:00:00

abstract：:Biological activities of five synthetic lipid A analogues (D-glucosamine derivatives) were examined to elucidate the structure required for expression of the biological activities of endotoxin. Proclotting enzyme of horseshoe crab activation, interferon-inducing and tumor necrosis factor-inducing activities were signi...

journal_title：FEBS letters

authors： Matsuura M,Kojima Y,Homma JY,Kubota Y,Yamamoto A,Kiso M,Hasegawa A

更新日期：1984-02-27 00:00:00

abstract：:Amylin, a 37 amino acid product of pancreatic beta-cells, inhibits insulin-stimulated radioglucose incorporation into muscle glycogen. In the current study, we exercised rats and then prelabelled the glycogen pool by infusing [3-3H]glucose along with glucose and insulin. Subsequent amylin administration increased the ...

journal_title：FEBS letters

authors： Young AA,Carlo P,Smith P,Wolfe-Lopez D,Pittner R,Wang MW,Rink T

更新日期：1993-11-22 00:00:00

abstract：:The dependence of protein splicing on conserved residues of the Cne PRP8 intein was assessed by alanine scanning mutagenesis in a foreign protein context. Corroboration was obtained for the involvement of residues at the splice junctions and of the conserved threonine and histidine of motif B. Five additional residues...

journal_title：FEBS letters

authors： Pearl EJ,Tyndall JD,Poulter RT,Wilbanks SM

更新日期：2007-06-26 00:00:00

abstract：:32P-Labelled washed rabbit platelets were incubated with 0.6 nM platelet activating factor (PAF-acether), giving a full aggregation and release response within 30-60 s. The major phospholipid changes observed under these conditions were: (1) An increased labelling of phosphatidic acid (PA) within 10 s and of phosphati...

journal_title：FEBS letters

authors： Mauco G,Chap H,Douste-Blazy L

更新日期：1983-03-21 00:00:00

abstract：:1-Farnesylpyridinium (FPy), an analog of isoprenoid farnesol, initially induced morphological changes similar to those of typical apoptosis in human leukemia HL-60 cells but FPy-treated cells were characterized by the absolute absence of final apoptotic events such as fragmentation into apoptotic bodies. FPy-induced c...

journal_title：FEBS letters

authors： Hamada M,Nishio K,Doe M,Usuki Y,Tanaka T

更新日期：2002-03-13 00:00:00

abstract：:Schizosaccharomyces pombe cells take up D-gluconate, as an alternative carbon source for growth, during glucose starvation or when cultured on glycerol-containing medium. Gluconate uptake is not detectable while cells are growing logarithmically on glucose. The addition of D-glucose as well as its non-metabolizable an...

journal_title：FEBS letters

authors： Caspari T,Urlinger S

更新日期：1996-10-21 00:00:00

abstract：:The effects of insulin and IGF-I on the cell surface quantities of GLUT1, GLUT3 and GLUT4 glucose transporters in L6 myotubes were determined with the exofacial bis-mannose phololabel (ATB-BMPA). In basal cells, an equal molar quantity of each transporter isoform was found at the cell surface. Insulin stimulated the t...

journal_title：FEBS letters

authors： Wilson CM,Mitsumoto Y,Maher F,Klip A

更新日期：1995-07-10 00:00:00

abstract：:Several protein kinase C (PKC) isozymes were analyzed by immunoblot and immunocytochemistry in cultures of hippocampal neurones at several stages of differentiation. Our findings reveal the existence of two distinct patterns of expression. Firstly, conventional PKC isozymes alpha, beta and gamma, that are expressed at...

journal_title：FEBS letters

authors： Tejero-Díez P,Rodríguez-Sánchez P,Díez-Guerra FJ

更新日期：1995-04-24 00:00:00

abstract：:Aquaporin-4, a predominant water channel in the central nervous system, has two isoforms, M1 and M23, whose transcripts are driven by distinct promoters. Using a reporter assay, we found that a fragment located between exons 0 and 1 of the mouse aquaporin-4 gene, which had been thought to be the promoter for M23, lack...

journal_title：FEBS letters

authors： Abe Y,Goda W,Ikeshima-Kataoka H,Yasui M

更新日期：2017-12-01 00:00:00

abstract：:The surprising result that the spanning domain causes retention of proteins in the Golgi stack poses the question as to the actual mechanism. Here we present a simple model that might have general applicability. ...

journal_title：FEBS letters

authors： Nilsson T,Slusarewicz P,Hoe MH,Warren G

更新日期：1993-09-06 00:00:00

abstract：:In this study we identify the molecules involved in the MAPK signal transduction pathway (Ras, Raf-1, Mek, Mek-P and MAPK) in highly purified endosomal fractions isolated from rat liver. Biochemical analysis shows that only the early-sorting endocytic compartment contains activated Raf-1 and Mek. Finally, the exogenou...

journal_title：FEBS letters

authors： Pol A,Calvo M,Enrich C

更新日期：1998-12-11 00:00:00

abstract：:A tropomyosin-binding protein (app. Mr 17000) was detected in equine platelet preparations by a gel overlay technique. Its isolation, amino acid and partial sequence analyses have shown it to be histone H2B. As with a similar protein from pig platelet preparations [der Terrossian et al. (1983) FEBS Lett. 152, 202-206]...

journal_title：FEBS letters

authors： Stewart DI,Golosinska K,Smillie LB

更新日期：1983-06-27 00:00:00

abstract：:Periplasmic adaptor proteins are essential components of bacterial tripartite multidrug efflux pumps. Here we report the 2.35 Å resolution crystal structure of the BesA adaptor from the spirochete Borrelia burgdorferi solved using selenomethionine derivatized protein. BesA shows the archetypal linear, flexible, multi-...

journal_title：FEBS letters

authors： Greene NP,Hinchliffe P,Crow A,Ababou A,Hughes C,Koronakis V

更新日期：2013-09-17 00:00:00

abstract：:Y RNAs are approximately 100 nucleotide long conserved cytoplasmic non-coding RNAs, which produce smaller RNA fragments during apoptosis. Here we show that these smaller RNA molecules are also produced in non-stressed cells and in a range of human cancerous and non-cancerous cell types. Recent reports have speculated ...

journal_title：FEBS letters

authors： Nicolas FE,Hall AE,Csorba T,Turnbull C,Dalmay T

更新日期：2012-04-24 00:00:00

abstract：:The covalent binding of pyridoxal 5'-phosphate (PLP) to human serum albumin (HSA) is important in the regulation of PLP metabolism. In plasma, PLP is bound to HSA at a single high-affinity and at two or more nonspecific sites. To characterize the primary PLP binding site, HSA was incubated with [3H] PLP, and the Schif...

journal_title：FEBS letters

authors： Bohney JP,Fonda ML,Feldhoff RC

更新日期：1992-02-24 00:00:00

abstract：:The cyclic AMP phosphodiesterases type 4 (PDE4s) are expressed in a cell specific manner, with intracellular targeting directed by unique N-terminal anchor domains. All long form PDE4s are phosphorylated and activated by PKA phosphorylation within their upstream conserved region 1 (UCR1). Here, we identify and charact...

journal_title：FEBS letters

authors： Byrne AM,Elliott C,Hoffmann R,Baillie GS

更新日期：2015-03-12 00:00:00

abstract：:Recent studies have revealed the presence of an atypical progesterone receptor on the plasma membrane of a relatively small subpopulation of human sperm cells. In this study, we show that the receptor is present in many cells in a latent form and that the receptor function in these cells is switched on asynchronously ...

journal_title：FEBS letters

authors： Mendoza C,Tesarik J

更新日期：1993-09-06 00:00:00

abstract：:Read-through or mutations of a stop codon resulting in translation of the 3'-UTR produce potentially toxic C-terminally extended proteins. However, quality control mechanisms for such proteins are poorly understood in mammalian cells. Here, a comprehensive analysis of the 3'-UTRs of genes associated with hereditary di...

journal_title：FEBS letters

authors： Hashimoto S,Nobuta R,Izawa T,Inada T

更新日期：2019-04-01 00:00:00

abstract：:Two hybrid proteins between Pseudomonas aeruginosa exotoxin A (PE) and poliovirus protease 2Apro have been generated. One hybrid protein contains the poliovirus 2Apro sequence replacing the region of PE corresponding to amino acids 413-607. The other hybrid contains in addition the transforming growth factor sequence....

journal_title：FEBS letters

authors： Novoa I,Feduchi E,Carrasco L

更新日期：1994-11-21 00:00:00

abstract：:To better understand the mechanism of intracellular Ca2+ mobilization, mouse oocytes were micro-injected with 'caged'-inositol-1,4,5 triphosphate caged-InsP3) together with the Ca2+ indicator Fluo-3 to directly induce and monitor Ca2+ redistribution. Photo-released InsP3 elicits [Ca2+]i changes exhibiting several kine...

journal_title：FEBS letters

authors： Peres A

更新日期：1990-11-26 00:00:00

abstract：:Open reading frame 67 of Bombyx mori nucleopolyhedrovirus (BmORF67) is a homologue of Autographa californica multiple NPV ORF81. The gene is conserved among all baculoviruses and is thus considered a baculovirus core gene. The transcript of BmORF67 was detected at 18-72 h post-infection (p.i.). Polyclonal antiserum ra...

journal_title：FEBS letters

authors： Chen HQ,Chen KP,Yao Q,Guo ZJ,Wang LL

更新日期：2007-12-22 00:00:00

abstract：:Detailed structural models of di-cluster seven-iron ferredoxins constitute a valuable resource for folding and stability studies relating the metal cofactors' role in protein stability. The here reported, hemihedric twinned crystal structure at 2.0 A resolution from Acidianus ambivalens ferredoxin, shows an integral 1...

journal_title：FEBS letters

authors： Frazão C,Aragão D,Coelho R,Leal SS,Gomes CM,Teixeira M,Carrondo MA

更新日期：2008-03-05 00:00:00