Abstract:
:Open reading frame 67 of Bombyx mori nucleopolyhedrovirus (BmORF67) is a homologue of Autographa californica multiple NPV ORF81. The gene is conserved among all baculoviruses and is thus considered a baculovirus core gene. The transcript of BmORF67 was detected at 18-72 h post-infection (p.i.). Polyclonal antiserum raised to a His-BmORF67 fusion protein recognized BmORF67 in infected cell lysates from 24 to 72 h p.i., suggesting that BmORF67 is a late gene. BmORF67 was not detected either in budded viruses or occlusion-derived virus. Immunofluoresence analysis showed that the protein located in the cytoplasm and interacted with host protein actin A3. In conclusion, BmORF67 is a late protein localized in the cytoplasm of infected cells that interacts with host protein.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Chen HQ,Chen KP,Yao Q,Guo ZJ,Wang LLdoi
10.1016/j.febslet.2007.11.059subject
Has Abstractpub_date
2007-12-22 00:00:00pages
5836-42issue
30eissn
0014-5793issn
1873-3468pii
S0014-5793(07)01210-0journal_volume
581pub_type
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