Abstract:
:Both HIV infection per se and antiretroviral drugs might contribute to oxidative stress and mitochondrial dysfunctions. In this study we assess zidovudine, stavudine and didanosine on U937 and CEM cell lines. All these drugs induced apoptosis and increased intracellular hydrogen peroxide but not superoxide anions. The addition of acetyl-l-carnitine (ALC) was able to prevent the pro-oxidant effect of the drugs tested. Supplementation with ALC, deficient in certain cohorts of HIV-infected individuals, especially on high active antiretroviral therapy regimen, has been associated with favourable effects. These data suggest that one of these effects could be a direct anti-oxidant action.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Ferraresi R,Troiano L,Roat E,Nemes E,Lugli E,Nasi M,Pinti M,Calvani M,Iannuccelli M,Cossarizza Adoi
10.1016/j.febslet.2006.11.016subject
Has Abstractpub_date
2006-12-11 00:00:00pages
6612-6issue
28-29eissn
0014-5793issn
1873-3468pii
S0014-5793(06)01332-9journal_volume
580pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The gatC, gatA and gatB genes encoding the three subunits of glutamyl-tRNA(Gln) amidotransferase from Acidithiobacillus ferrooxidans, an acidophilic bacterium used in bioleaching of minerals, have been cloned and expressed in Escherichia coli. As in Bacillus subtilis the three gat genes are organized in an operon-like...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02600-x
更新日期:2001-07-06 00:00:00
abstract::A latent MAP kinase kinase kinase activity previously detected after chromatography of PC12 cell extracts on Mono Q [(1992) FEBS Lett. 314, 461-465] has been identified as B-Raf by immunoblotting and immunoprecipitation experiments and by its specific activation with B-Raf antibodies. B-Raf is phosphorylated after sti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00723-3
更新日期:1994-08-15 00:00:00
abstract::Acetylcholinesterase (AChE) is likely to have roles other than the hydrolysis of acetylcholine, e.g., related to developmental processes like neurite outgrowth, differentiation and adhesion. Here, we investigated whether AChE can function as a heterophilic cell adhesion molecule and searched for proteins interacting w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.08.078
更新日期:2004-10-08 00:00:00
abstract::A new assay for the calcium channel activity of annexin V was developed. The calcium-sensitive fluorescence indicator, fura-2, was incorporated into large unilamellar vesicles (LUV). After establishing a calcium gradient across the liposomal membranes, native or mutated annexin V was added. The resulting calcium influ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81507-v
更新日期:1993-02-08 00:00:00
abstract::This study was undertaken to demonstrate the unique specificity of the chemokine receptor CXCR4 antagonist AMD3100. Calcium flux assays with selected chemokine/cell combinations, affording distinct chemokine receptor specificities, revealed no interaction of AMD3100 with any of the chemokine receptors CXCR1 through CX...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03143-5
更新日期:2002-09-11 00:00:00
abstract::Lipids are essential eukaryotic cellular constituents. Lipid metabolism has a strong impact on cell physiology, and despite good progress in this area, many important basic questions remain unanswered concerning the functional diversity of lipid species and on the mechanisms that cells employ to sense and adjust their...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2012.07.033
更新日期:2012-08-31 00:00:00
abstract::Single rat hepatocytes microinjected with aequorin show free Ca oscillations when stimulated with Ca(2+)-mobilizing hormones. We show here that an inhibitor of diacylglycerol kinase (R 59 022) and an analogue of native diacylglycerol (diC8) inhibit free Ca oscillations induced by phenylephrine and vasopressin. These r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81409-f
更新日期:1992-11-02 00:00:00
abstract::Engrailed-2 (En-2) belongs to an evolutionarily conserved family of DNA binding homeodomain-containing proteins that are expressed in mammalian brain during development. Here, we demonstrate that serine 267 in the homeodomain of En-2 is phosphorylated by protein kinase A (PKA) in forskolin-treated COS-7 cells. Further...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.05.009
更新日期:2004-06-18 00:00:00
abstract::Fas ligand (FasL) binds Fas (CD95) to induce apoptosis or activate other signaling pathways. In addition, FasL transduces bidirectional or 'reverse signals'. The intracellular domain of FasL contains consensus sequences for phosphorylation and an extended proline rich region, which regulate its surface expression thro...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.08.025
更新日期:2007-09-18 00:00:00
abstract::Three single-stranded RNA transcripts synthesized in vitro by a virion-associated RNA polymerase of bacteriophage phi 6 were sequenced at their 5'- and 3'-termini. The sequences agreed with those of the + strands of the 3 double-stranded RNA segments [FEBS Lett. (1982) 141,111-115]. The results show that the transcrip...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(82)80815-6
更新日期:1982-11-08 00:00:00
abstract::Increased expression of low voltage-activated, T-type Ca(2+) channels has been correlated with a variety of cellular events including cell proliferation and cell cycle kinetics. The recent cloning of three genes encoding T-type alpha(1) subunits, alpha(1G), alpha(1H) and alpha(1I), now allows direct assessment of thei...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01832-9
更新日期:2000-07-28 00:00:00
abstract::Treatment of yeast phosphoglycerate kinase (PGK) with trypsin results in a fourfold increase in the Vmax of this enzyme, without affecting the Km. This activation is shown to be due to the removal of the C-terminal lysine residue. The C-terminal sequence folds back over the N-terminal domain and contacts the extreme N...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00348-1
更新日期:1996-04-29 00:00:00
abstract::A bovine anterior pituitary polypeptide that stimulates the replication of rat and human adipocyte precursors has been purified. Its Mr is 44 000-53 000 and its isoelectric point is 9.8-10.3. While pituitary basic fibroblast growth factor is equally mitogenic on adipocyte precursors and skin fibroblasts, the polypepti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80651-6
更新日期:1983-03-21 00:00:00
abstract::The carboxy-terminal half of the c-src protein fused to the protein A moiety was expressed in bacteria. The protein A/truncated c-src fusion protein, which does not have SH2 and SH3 domains, is found in the periplasmic space allowing for a simple one-step purification and demonstrated high efficiency in autophosphoryl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80174-s
更新日期:1993-07-26 00:00:00
abstract::Islet amyloid polypeptide (IAPP) is the 37-amino acid peptide subunit of amyloid found in pancreatic islets of type 2 diabetic patients and in insulinomas. Recently, we isolated the human gene encoding IAPP [(1988) FEBS Lett. 239, 227-232]. We now report the nucleotide sequences of a human insulinoma cDNA encoding a c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81260-8
更新日期:1989-04-10 00:00:00
abstract::Keratins form an intracellular keratin filament network in keratinocytes. Point mutations in the epidermal keratins could lead to the disruption of keratin filament formation, developing skin diseases such as epidermolytic hereditary palmoplantar keratoderma (EHPPK). We found a G to A transition in keratin 9 (K9) cDNA...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00393-6
更新日期:1996-05-20 00:00:00
abstract::Superoxide reacts with nitric oxide to form peroxynitrite, a potent oxidising agent which may contribute to tissue damage in pathological situations such as inflammation and ischaemia/reperfusion. One mechanism by which oxidative stress damages tissues is the induction of a specific Cyclosporin A-sensitive mitochondri...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00461-7
更新日期:1994-05-30 00:00:00
abstract::Previous electrophysiological experiments characterized the Sec61 complex, which provides the aqueous path for entry of newly-synthesized polypeptides into the mammalian endoplasmic reticulum, as a highly dynamic channel that, once activated by precursor proteins, fluctuates between main open states with mean conducta...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.06.032
更新日期:2009-07-21 00:00:00
abstract::The gas phase of the soil plays an important role in plant growth and development. We investigated the effect of rhizospheric NO as a signalling compound for N uptake of beech roots. Following exposure to NO, ammonium and glutamine uptake into roots were determined using (15)N-labelling, and gene expression of selecte...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.07.052
更新日期:2009-09-03 00:00:00
abstract::The phosphatidylinositol 4,5-bisphosphate (PtdIns-P2)-regulated actin-binding protein gelsolin and most phosphoinositide-specific phospholipases C (PLCs) comprise a basic amino acid motif ((K/R)xxxKxK(K/R); x denotes any amino acid) which was previously suggested to represent a PtdInsP2-binding site commonly present i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00464-k
更新日期:1995-05-29 00:00:00
abstract::Several recombinant proteins in inclusion bodies expressed in Escherichia coli have been measured by Fourier transform infrared and solid-state nuclear magnetic resonance spectra to provide the secondary structural characteristics of the proteins from hyperthermophilic archaeon Pyrococcus horikoshii OT3 (hyperthermoph...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01441-8
更新日期:2004-01-16 00:00:00
abstract::The crystal structure of metagenome-derived LC9-RNase H1 was determined. The structure-based mutational analyses indicated that the active site motif of LC9-RNase H1 is altered from DEDD to DEDN. In this motif, the location of the second glutamate residue is moved from αA-helix to β1-strand immediately next to the fir...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.03.020
更新日期:2013-05-02 00:00:00
abstract::RNA binding motif protein 5 (RBM5) is a candidate tumor suppressor gene. Recent studies showed that RBM5 functions as an alternative splicing regulator of apoptosis-related genes. Here, we identify DHX15 and PRP19, two spliceosome components, as novel RBM5-interacting partners. We then show that the G-patch domain of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.02.052
更新日期:2012-04-05 00:00:00
abstract::Rhizobium sigma vegetative-dependent promoters are different from those of enteric bacteria and have never been characterized before. We report here the biochemical characterization of the ORF1-ntrBC promoter of Rhizobium etli. The minimal promoter region was located by means of a transcriptional fusion and further ch...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00507-8
更新日期:1996-06-10 00:00:00
abstract::The interactions between clavulanate and three class C enzymes have been studied in detail. In all cases, the reactions followed branched pathways where 25-150 turnovers occurred before inactivation was completed. Reactivation rates were quite low. The poor efficiency of clavulanate as a class C inactivator appeared t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80692-n
更新日期:1993-11-22 00:00:00
abstract::Synaptotagmins (Syts) are involved in Ca(2+)-dependent insulin release. However, which Syt isoform is functional in primary beta-cells remains unknown. We demonstrate by electron microscopy of pancreatic islets, the association of Syt 9 with insulin granules. Silencing of Syt 9 by RNA interference adenovirus in islet ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.08.047
更新日期:2005-09-26 00:00:00
abstract::The effective synthesis of the envelope antigen F1 of Y. pestis in E. coli HB101 is mediated by the expression of the caf1M gene. This gene was sequenced, and the protein encoded was found to have a significant homology with the chaperone protein PapD of uropathogenic E. coli. The data presented allow one to suppose C...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80945-y
更新日期:1991-07-29 00:00:00
abstract::The operation of the human red cell glucose transporter has been studied at normal and high hydrostatic pressure to identify the step(s) which involve a volume change. Pressure inhibited zero-trans and equilibrium exchange influx to similar extents, by decreasing the Vmax but not significantly changing the Km. The Bma...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80261-e
更新日期:1992-04-27 00:00:00
abstract::During steady-state, the Pi released in the medium is derived from glucose-6-phosphate which continuously regenerates the ATP hydrolyzed. A membrane potential (delta psi) can be built up in submitochondrial particles using glucose-6-phosphate and hexokinase as an ATP-regenerating system. The energy derived from the me...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81273-o
更新日期:1992-08-17 00:00:00
abstract::In Alzheimer's disease, beta-amyloid peptides (betaA(1-40) and betaA(1-42)) are deposited on the brain cell surfaces as neurotoxic plaques. Some reports indicate that small heat shock proteins, Hsp27 and alphaB-crystallin, colocalize in the plaques, but their functions are not known. Interaction between betaA and alph...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02136-0
更新日期:2000-11-03 00:00:00