Abstract:
:Targeting gene therapy vectors to abundant receptors on airway epithelia may allow a significant enhancement of gene delivery and thereby be of particular importance for the gene therapy of cystic fibrosis. Alpha9beta1-integrins are highly expressed throughout the human airway epithelia in vivo, irrespective of any particular clinical status. Aiming to improve the targeting of our non-viral integrin-mediated gene transfer systems to airway epithelia, we searched for a short tenascin C-derived peptide which would bind to these integrins. By utilizing recombinant bacteriophages that display overlapping regions of the third fibronectin type III repeat of tenascin C (TNfn3), we were able to localize its alpha9beta1-integrin binding site to the B-C loop of TNfn3. A synthetic Pro-Leu-Ala-Glu-Ile-Asp-Gly-Ile-Glu-Leu-Thr-Tyr peptide (PLAEIDGIELTY) was shown to displace alpha9beta1-integrin-expressing cells completely from binding to TNfn3. This peptide, therefore, may prove useful both for the examination of the functional importance of alpha9beta1-integrins in vivo and the development of gene therapy vectors or drugs targeting these integrins.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Schneider H,Harbottle RP,Yokosaki Y,Kunde J,Sheppard D,Coutelle Cdoi
10.1016/s0014-5793(98)00612-7subject
Has Abstractpub_date
1998-06-16 00:00:00pages
269-73issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(98)00612-7journal_volume
429pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The solution conformation of the cardiac stimulatory sea anemone polypeptide anthopleurin-A has been characterised using distance geometry and restrained molecular dynamics calculations. A set of 253 approximate interproton distance restraints and 14 peptide backbone torsion angle restraints derived from two-dimension...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80931-1
更新日期:1988-11-07 00:00:00
abstract::The inhibition of electron transport by unnatural (-)-antimycin A3 was examined with rat liver mitochondria and compared with that of natural (+)-antimycin A3. (-)-Antimycin A3 inhibited respiration about 1/100th as strongly as natural (+)-antimycin A3. (-)-Antimycin A3 binding to the cytochrome bc1 complex did not se...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80834-p
更新日期:1991-11-04 00:00:00
abstract::Leonor Michaelis spent the years of 1922-1926 as Professor of Biochemistry of the Aichi Medical College (now Graduate School of Medicine, Nagoya University) in Nagoya, Japan. Michaelis succeeded in gathering many bright young biochemists from all over Japan into his laboratory, and made tremendous contributions to the...
journal_title:FEBS letters
pub_type: 传,历史文章,杂志文章
doi:10.1016/j.febslet.2013.04.020
更新日期:2013-09-02 00:00:00
abstract::Creutzfeldt-Jakob disease is a slow, infectious, progressive neurological disorder which results in human dementia. Synaptic membranes from various brain regions of guinea pigs infected with Creutzfeldt-Jakob disease show increased guanyl nucleotide- or 5-hydroxytryptamine-mediated activation of adenylate cyclase. Thi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)81205-4
更新日期:1986-03-17 00:00:00
abstract::Four 12.2-12.6 kDa small heat-shock proteins (sHSPs) of Caenorhabditis elegans are the smallest known members of the sHSP family. They essentially comprise the characteristic C-terminal 'alpha-crystallin domain' of the sHSPs, having a very short N-terminal region, and lacking a C-terminal tail. Recombinant Hsp12.2 and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00917-x
更新日期:1998-08-21 00:00:00
abstract::Extensive data on the glycosaminoglycan (GAG) composition and the collagen fibril diameter distribution have been collected for a diverse range of connective tissues. It is shown that tissues with the smallest diameter collagen fibrils (mass-average diameter less than 60 nm) have high concentrations of hyaluronic acid...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(82)81060-0
更新日期:1982-11-22 00:00:00
abstract::Inborn errors of the urea cycle, liver malfunction and drug-induced hepatotoxicity are causes of life-threatening encephalopathies arising from hyperammonemia. L-Carnitine prevented entirely ammonia toxicity in mice when injected intraperitoneally 30 min before a lethal dose of ammonium acetate. Survival depends on th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80106-4
更新日期:1984-01-30 00:00:00
abstract::In HCT116 colorectal cancer cells, HeLa cervical cancer cells and HuH-7 hepatoma cells, miR-223 is expressed at a low level. Through infection with lentivirus containing miR-223 precursor, miR-223 [corrected] was overexpressed in all these cells. Interestingly, the expression levels of FOXO1 mRNA and protein, and phos...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.02.050
更新日期:2012-04-05 00:00:00
abstract::Polyclonal antibodies have been raised against two synthetic peptides reproducing the 48-64 and 353-369 sequences of CSK, a protein tyrosine kinase implicated in the down-regulation of src-related protein kinases. Both antibodies specifically recognize recombinant CSK and a CSK-related 49 kDa protein tyrosine kinase p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81212-5
更新日期:1992-11-30 00:00:00
abstract::Dinucleotide polyphosphates (ApnA) have emerged as signaling molecules in rapidly dividing cells. The presence and role of Ap5A in the heart remain unknown. Here, we report that the myocardium contains abundant amounts of diadenosine 5',5"-P1,P5-pentaphosphate (Ap5A), a member of the ApnA family. Ischemia induced 10-f...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00114-8
更新日期:1998-02-27 00:00:00
abstract::We have previously shown that Rap1 as well as its guanine nucleotide exchange factor Epac1 increases cell-cell junction formation. Here, we show that activation of Epac1 with the exchange protein directly activated by cAMP (Epac)-specific cAMP analog 8CPT-2'O-Me-cAMP (007) resulted in a tightening of the junctions and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.07.080
更新日期:2005-09-12 00:00:00
abstract::Interactions between Trypanosoma cruzi and the extracellular matrix play an important role in cellular invasion. Here we show that T. cruzi increases the levels of thrombospondin-1 (TSP-1) expression in host cells during early infection. Stable RNA interference of host cell TSP-1 knocks down the levels of TSP-1 transc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.03.054
更新日期:2006-04-17 00:00:00
abstract::The effect on cytosolic Ca2+ concentration ([Ca2+]i) of cAMP analogues and the adenylate cyclase-stimulating agents forskolin, isoproterenol and glucagon has been examined in an insulin-secreting beta-cell line (HIT T-15) using fura 2. All these manipulations of the cAMP messenger system promoted a rise in [Ca2+]i whi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80884-0
更新日期:1987-08-10 00:00:00
abstract::We have examined the activity of protein disulfide isomerase (PDI) and glutaredoxin (Grx) 1, 2 and 3 from Escherichia coli to catalyze the cleavage of glutathionylated ribonuclease A (RNase-SG) by 1 mM GSH to yield reduced RNase. Apparent Km values for RNase-SG were similar, 2-10 microM, for Grx 1, 3 and PDI but Grx I...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01698-6
更新日期:1999-01-25 00:00:00
abstract::Secretoneurin is a recently characterized neuropeptide present in the primary amino acid sequence of secretogranin II. We investigated the proteolytic processing of secretogranin II by prohormone convertases in vivo in a cellular system using the vaccinia virus system. Both PC1 and PC2 can cleave the secretogranin II ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00127-u
更新日期:1995-03-06 00:00:00
abstract::Malignant mesothelioma (MM) is a fatal type of cancer. We studied the role of the redox-active protein thioredoxin-1 (Trx-1) in apoptosis induced in MM cells and their non-malignant counterparts (Met-5A) by alpha-tocopheryl succinate (alpha-TOS) and TNF-related apoptosis-inducing ligand (TRAIL). MM cells were suscepti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.04.019
更新日期:2006-05-15 00:00:00
abstract::The functional molecular masses of the vacuolar membrane H+-ATPase in Saccharomyces cerevisiae under two kinetic conditions for ATP hydrolysis were measured by radiation inactivation. When vacuolar membrane vesicles were exposed to gamma-rays from 60Co, the activities catalyzing a single-cycle and multi-cycles of ATP ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80571-x
更新日期:1989-02-27 00:00:00
abstract::The thermal stability of PQQ glucose dehydrogenases (PQQGDHs) which were chimeras with more than 95% made up of the N-terminal region of Escherichia coli PQQGDH and the rest made up of the C-terminal region of Acinetobacter calcoaceticus PQQGDH was investigated. Among the chimeric PQQGDHs, E97A3 (E. coli 97% and A. ca...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00418-9
更新日期:1995-05-15 00:00:00
abstract::Various vertebrates use ultraviolet (UV) vision for such basic behaviors as mating, foraging, and predation. We have successfully interchanged the color-sensitivities of the mouse UV pigment and the human blue pigment by introducing forward and reverse mutations at five sites. This unveils for the first time the gener...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02269-9
更新日期:2000-12-08 00:00:00
abstract::Methylation of cytosine-residues in the sequence CpG affects the expression of many genes and generally correlates with reduced transcription. The ribosomal genes of Xenopus laevis were among the first genes to be studied with respect to their DNA methylation, and a loss of methylation during embryonic development cor...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01291-1
更新日期:1994-12-19 00:00:00
abstract::Recombinant cell lines expressing D2 and D3 dopamine receptor isoforms have been used to study coupling of these receptors to G-proteins from the effects of GTP to reduce agonist binding affinities in agonist/[3H]spiperone competition experiments. D2(short) expressed in Ltk- cells, D2(long) expressed in Ltk- or CHO ce...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81168-y
更新日期:1993-01-11 00:00:00
abstract::The complete amino acid sequence of prostaglandin endoperoxide synthase from sheep vesicular gland has been deduced by cloning and sequence analysis of DNA complementary to its messenger RNA. The results were confirmed by digestion of the enzyme with carboxypeptidase Y and by automated Edman degradation of the intact ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80847-0
更新日期:1988-04-25 00:00:00
abstract::We characterized the promoter activity of a medaka fish intestinal guanylyl cyclase gene, OlGC6, by assay of enzyme activity in response to various promoter-luciferase fusion gene constructs introduced into CACO-2 cells and medaka fish embryos. A transient transfection assay of the various fusion gene constructs showe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00002-4
更新日期:2003-02-11 00:00:00
abstract::Alix and its homologs are involved in various phenomena such as endosomal protein-sorting and adaptation to stress conditions. In this study, we found that development of Dictyostelium discoideum Alix (DdAlix) deletion mutant (alx-) cells was impaired in alkaline pH environments. The fruiting body formation efficiency...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.02.036
更新日期:2005-03-14 00:00:00
abstract::A recombinant antigen (p22-NS3), possessing putative HCV nucleocapsid protein (p22) and non-structural protein 3 (NS3) epitopes, was heavily expressed in E. coli and purified. The p22-NS3 purified recombinant antigen strongly reacts with sera containing human antibodies directed against p22 and NS3 providing a startin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80128-h
更新日期:1993-06-21 00:00:00
abstract::The nitration of tyrosine residues in protein occurs through the action of reactive oxygen and nitrogen species and is considered a marker of oxidative stress under pathological conditions. The most active nitrating species so far identified is peroxynitrite, the product of the reaction between nitric oxide and supero...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02726-0
更新日期:2001-08-17 00:00:00
abstract::Previously determined protein structures have been analysed, in order to find folding motifs similar to that proposed by NMR spectroscopy, for the N-terminal, 14 kDa fragment of the Ada protein. The analyses reveal only limited similarities with the NMR-derived structural data and strongly suggest that this region of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81352-z
更新日期:1993-06-01 00:00:00
abstract::More than 40 years have passed since the original publication by Hayflick and Moorhead led to the concept of the 'Hayflick limit' of the maximum number of divisions which somatic cells undergo in vitro. This concept is still regarded as a fundamental characteristic of species longevity. Here we want to emphasize anoth...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(03)00298-9
更新日期:2003-04-24 00:00:00
abstract::Activation of the signal transducer and activator of transcription 3 (STAT3) in response to interleukin-6 (IL-6) type cytokines involves both phosphorylation of Tyr705, which enables dimerization, nuclear translocation and DNA binding, as well as ser727 phosphorylation. Here, we describe that the 65 C-terminal amino a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02354-7
更新日期:2001-04-20 00:00:00
abstract::The sequences of rat testis carbonyl reductase (rCR1) and rat ovary carbonyl reductase (rCR2) are 98% identical, differing only at amino acids 140, 141, 143, 235 and 238. Despite such strong sequence identity, we find that rCR1 and rCR2 have different catalytic constants for metabolism of menadione and 4-benzoyl-pyrid...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.11.049
更新日期:2006-01-09 00:00:00