Abstract:
:The solution conformation of the cardiac stimulatory sea anemone polypeptide anthopleurin-A has been characterised using distance geometry and restrained molecular dynamics calculations. A set of 253 approximate interproton distance restraints and 14 peptide backbone torsion angle restraints derived from two-dimensional 1H-NMR spectra at 500 MHz were used as input for these calculations. 13 structures generated by either metric matrix or variable target function distance geometry calculations were refined using energy minimisation and restrained molecular dynamics. The resulting structures contain a region of twisted antiparellel beta-sheet to which two separate regions of unordered chain are linked by three disulphide bonds. Two loops, one including Pro-41 and the other encompassing residues 10-18, are poorly defined by the NOE data.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Torda AE,Mabbutt BC,van Gunsteren WF,Norton RSdoi
10.1016/0014-5793(88)80931-1subject
Has Abstractpub_date
1988-11-07 00:00:00pages
266-70issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(88)80931-1journal_volume
239pub_type
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