Abstract:
:We describe the creation of cell adhesion mediated by cell surface engineering. The Flt3-ligand was fused to a membrane anchor made of the diphtheria toxin translocation domain. The fusion protein was attached to the surface of a cell by an acid pulse. Contact with another cell expressing the receptor Flt3 lead to its activation. This activity involved direct cell-cell contact. A mean force of 20 nN was needed to separate functionalized cells after 5 min of contact. Overall, we showed that it is possible to promote specific cell-cell adhesion by attaching protein ligands at the surface of cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Perier A,Gourier C,Pichard S,Husson J,Lajeunesse E,Babon A,Menez A,Gillet Ddoi
10.1016/j.febslet.2007.10.055subject
Has Abstractpub_date
2007-11-27 00:00:00pages
5480-4issue
28eissn
0014-5793issn
1873-3468pii
S0014-5793(07)01123-4journal_volume
581pub_type
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