Abstract:
:The Ncx gene encodes a homeobox containing transcription factor that belongs to the Hox11 gene family. We determined specific Ncx protein binding consensus DNA sequences. Optimal Ncx binding sequences were 5'-CGGTAATTGG-3' (TAAT core) and 5'-CGGTAAGTGG-3' (TAAG core), which coincided with the Hox11 binding sequence. Both Ncx and Hox11 could bind to the TAAT and the TAAG core oligonucleotide in vitro. However, they could efficiently transactivate the reporter plasmid linked to the TAAT core sequence but not to the TAAG core sequence. Thus, Ncx and Hox11 act as transcriptional activators via their target sequence, 5'-CGGTAATTGG-3'.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Shimizu H,Kang M,Iitsuka Y,Ichinose M,Tokuhisa T,Hatano Mdoi
10.1016/s0014-5793(00)01651-3keywords:
subject
Has Abstractpub_date
2000-06-23 00:00:00pages
170-4issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(00)01651-3journal_volume
475pub_type
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