Abstract:
:Pigeon liver malic enzyme (EC 1.1.1.40) has a double dimer quaternary structure. The NADP+ analogs, aminopyridine adenine dinucleotide phosphate and nicotinamide-1,N6-ethenoadenosine dinucleotide phosphate, bind to the enzyme anti-cooperatively. In the presence of non-cooperative competing ligand NADP+, the binding parameter Hill coefficients of these analogues changed very little. Binding of L-malate with enzyme-AADP+ complex first enhanced then reduced the nucleotide fluorescence. Two L-malate binding sites, with Kd values of 23-30 and 270-400 microM, respectively. for the tight and weak binding sites were postulated. A hybrid model between the sequential and pre-existing asymmetrical models was proposed for the pigeon liver malic enzyme.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Lee HJ,Chang GGdoi
10.1016/0014-5793(90)80837-9subject
Has Abstractpub_date
1990-12-17 00:00:00pages
175-9issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(90)80837-9journal_volume
277pub_type
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