Abstract:
:The black-eyed pea trypsin and chymotrypsin inhibitor (BTCI) is a member of the Bowman-Birk protease inhibitor (BBI) family. The three-dimensional model of the BTCI-chymotrypsin complex was built based on the homology to Bowman-Birk inhibitors with known structures. An extensive theoretical and experimental study of these known structures has been performed. The model confirms the ideas about Bowman-Birk inhibitor structure-function relations and agrees well with our experimental data (circular dichroism, IR and light scattering). The electrostatic potentials at the enzyme-inhibitor contact surface reveal a pattern of complementary electrostatic potentials from which mutations can be inferred that could give these inhibitors an altered specificity.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
de Freitas SM,de Mello LV,da Silva MC,Vriend G,Neshich G,Ventura MMdoi
10.1016/s0014-5793(97)00419-5subject
Has Abstractpub_date
1997-06-09 00:00:00pages
121-7issue
2eissn
0014-5793issn
1873-3468pii
S0014-5793(97)00419-5journal_volume
409pub_type
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