Abstract:
:Integrin avidity is regulated by changes in the conformation of the heterodimer and cluster formation. We measured cell adhesion by integrin alpha2beta1 (CHO-alpha2) to collagen at short contact times (0.5-60s) by single cell force spectroscopy (SCFS). The adhesion increased rapidly with contact time and was further strengthened by the addition of 12-O-tetradecanoylphorbol-13-acetate (TPA), a protein kinase C (PKC) and integrin activator. TPA also improved the strength of adhesive units. Furthermore, changes in membrane nanotube properties indicated better coupling of integrins to the cell cytoskeleton. We conclude that in addition to increasing integrin avidity TPA strengthens integrin-cytoskeletal linkage.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tulla M,Helenius J,Jokinen J,Taubenberger A,Müller DJ,Heino Jdoi
10.1016/j.febslet.2008.09.022subject
Has Abstractpub_date
2008-10-15 00:00:00pages
3520-4issue
23-24eissn
0014-5793issn
1873-3468pii
S0014-5793(08)00756-4journal_volume
582pub_type
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