Escherichia coli tRNA 2-selenouridine synthase (SelU) converts S2U-RNA to Se2U-RNA via S-geranylated-intermediate.

abstract：:Localisation of mRNA encoding the four AMPA glutamate receptor subunits in the rat adrenal gland was investigated using in situ hybridisation. GluR1 is found in the zona glomerulosa of the cortex, GluR3 in the remaining parts of the cortex, GluR2 in adrenal medullary cells and GluR4 at a very low level in the zona glo...

journal_title：FEBS letters

authors： Kristensen P

更新日期：1993-10-11 00:00:00

abstract：:In order to identify physiological activators of proteinase-activated receptor-2 (PAR-2), a peptide chloromethane inhibitor (biotinyl-Ser-Lys-Gly-Arg-CH2Cl) based on the cleavage site for activation of PAR-2 was synthesised and tested with 12 trypsin-like serine proteinases. The second-order rate constant (ki/Ki) for ...

journal_title：FEBS letters

authors： Fox MT,Harriott P,Walker B,Stone SR

更新日期：1997-11-17 00:00:00

abstract：:The oleosin glycine-rich protein genes Atgrp-6, Atgrp-7, and Atgrp-8 occur in clusters in the Arabidopsis genome and are expressed specifically in the tapetum cells. The cis-regulatory regions involved in the tissue-specific gene expression were investigated by fusing different segments of the gene cluster to the uidA...

journal_title：FEBS letters

authors： Franco LO,de O Manes CL,Hamdi S,Sachetto-Martins G,de Oliveira DE

更新日期：2002-04-24 00:00:00

abstract：:Tyrosyl radicals can catalyze LDL oxidation. In addition to their LDL oxidizing ability, superoxide (O2.-)/nitric oxide (NO.) generate phenoxyl radicals when reacting with tyrosine. Therefore we tested if tyrosine can act as a pro-oxidant in O2.-/NO.-initiated LDL oxidation. When LDL was exposed to O2.-/NO., tyrosine ...

journal_title：FEBS letters

authors： Kapiotis S,Hermann M,Held I,Mühl A,Gmeiner B

更新日期：1997-06-09 00:00:00

abstract：:We present here a new set of aminoglycoside-arginine conjugates (AACs) that are either site-specific or per-arginine conjugates of paromomycin, neamine, and neomycin B as well as their structure-activity relationships. Their binding constants (KD) for TAR and RRE RNAs, measured by fluorescence anisotropy, revealed dep...

journal_title：FEBS letters

authors： Lapidot A,Vijayabaskar V,Litovchick A,Yu J,James TL

更新日期：2004-11-19 00:00:00

abstract：:Structural analogs of adenosine 5'-acylphosphates, which are intermediates of the reaction catalyzed by acyl-CoA synthetases, were synthesized by condensing primary alcohols with AMP to examine the inhibitory effects on the lignoceroyl-CoA and palmitoyl-CoA synthetase activities. Hexadecyl, octadecyl, eicosyl, docosyl...

journal_title：FEBS letters

authors： Shiraishi T,Tezuka K,Uda Y

更新日期：1994-10-03 00:00:00

abstract：:Carbohydrate chains of C1-inhibitor were identified with a binding assay using different lectins. Lectins from Sambucus nigra (SNA) and Maackia amurensis (MAA) that are specific for sialic acids bound to C1-inhibitor. Lectin from Datura stramonium (DSA) reacted also with the inhibitor indicating complex and hybrid sug...

journal_title：FEBS letters

authors： Schoenberger OL

更新日期：1992-12-21 00:00:00

abstract：:In cardiac cells the effects of n-3 PUFAs on the whole genome are still unknown despite their recognized cardioprotective effects and ability to modulate gene expression. We have evaluated the effects of n-3 PUFAs supplementation on the global gene expression profile in cultured neonatal rat cardiomyocytes, detecting ...

journal_title：FEBS letters

authors： Bordoni A,Astolfi A,Morandi L,Pession A,Danesi F,Di Nunzio M,Franzoni M,Biagi P,Pession A

更新日期：2007-03-06 00:00:00

abstract：:The mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes syndrome (MELAS) is a rare congenital disorder of mitochondrial DNA. Five single nucleotide substitutions within the human mitochondrial tRNALeu(UUR) gene have been reported to be associated with MELAS. Here, we provide in vitro evide...

journal_title：FEBS letters

authors： Hao R,Yao YN,Zheng YG,Xu MG,Wang ED

更新日期：2004-12-03 00:00:00

abstract：:The polyketide synthase associated with the biosynthesis of enediyne-containing calicheamicin contains a putative phosphopantetheinyl transferase (PPTase) domain. By cloning and expressing the C-terminal region of the polyketide synthase and in vitro phosphopantetheinylation assay, we found that the PPTase domain exhi...

journal_title：FEBS letters

authors： Murugan E,Liang ZX

更新日期：2008-04-02 00:00:00

abstract：:All acetylcholine receptor subunit genes contain E boxes and are blocked by membrane depolarization. We have used transfected C2C12 myogenic cells to investigate the response, to electrical stimulation and KCl, of wildtype and mutant regulatory regions of the chick acetylcholine receptor alpha, gamma and delta subunit...

journal_title：FEBS letters

authors： Su CT,Huang CF,Schmidt J

更新日期：1995-06-12 00:00:00

abstract：:Oligopeptidase B (OpdB) from Trypanosoma brucei is a candidate therapeutic target in African trypanosomiasis. OpdB is an atypical serine peptidase, since activity is inhibited by thiol-blocking reagents and enhanced by reducing agents. We have identified C256 as the reactive cysteine residue that mediates OpdB inhibit...

journal_title：FEBS letters

authors： Morty RE,Shih AY,Fülöp V,Andrews NW

更新日期：2005-04-11 00:00:00

abstract：:Cytotoxic effects were observed following 4 h incubation of human leukaemic cells with the iron chelator 1-hydroxypyridine-2-thione (omadine). Its cytotoxic activity was comparable to that of the cytotoxic drug doxorubicin. At the same concentration two other effective iron chelators, desferrioxamine and 1,2-dimethyl-...

journal_title：FEBS letters

authors： Kontoghiorghes GJ,Piga A,Hoffbrand AV

更新日期：1986-08-18 00:00:00

abstract：:Phosphorylation of histone variant H2AX at serine 139, named gammaH2AX, has been widely used as a sensitive marker for DNA double-strand breaks (DSBs). gammaH2AX is required for the accumulation of many DNA damage response (DDR) proteins at DSBs. Thus it is believed to be the principal signaling protein involved in DD...

journal_title：FEBS letters

authors： Yuan J,Adamski R,Chen J

更新日期：2010-09-10 00:00:00

abstract：:The myo-inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ mobilization in single saponin-permeabilized and fura-2-loaded parietal cells was analysed by a fluorescence digital image processor. When the cells were incubated with ATP, free cytoplasmic Ca2+ concentration [( Ca2+]i) increased in some restricted cytoplasmic r...

journal_title：FEBS letters

authors： Tsunoda Y,Yodozawa S,Tashiro Y

更新日期：1988-04-11 00:00:00

abstract：:The -proteobacterium Wolinella succinogenes grows anaerobically by respiratory nitrite ammonification but not by denitrification. Nevertheless, it is capable of N(2)O reduction to N(2). Recently, the genome sequence of W. succinogenes revealed a nos gene cluster with intriguing features encoding a new type of N(2)O re...

journal_title：FEBS letters

authors： Simon J,Einsle O,Kroneck PM,Zumft WG

更新日期：2004-07-02 00:00:00

abstract：:Using the patch-clamp technique, we demonstrate here the opening of K+ channels evoked by the actively transported amino acid L-alanine in isolated Necturus enterocytes. These channels had a conductance of about 30 pS and their activation was dependent on transmembrane electrical potential and cytosolic Ca2+. ...

journal_title：FEBS letters

authors： Sheppard DN,Giraldez F,Sepúlveda FV

更新日期：1988-07-18 00:00:00

abstract：:The uncoupling protein-3 (UCP3) is a mitochondrial protein expressed mainly in skeletal muscle. Among several hypotheses for its physiological function, UCP3 has been proposed to prevent excessive production of reactive oxygen species. In the present study, we evaluated the effect of an oxidative stress induced by hyp...

journal_title：FEBS letters

authors： Flandin P,Donati Y,Barazzone-Argiroffo C,Muzzin P

更新日期：2005-06-20 00:00:00

abstract：:The bchM gene of Rhodobacter sphaeroides has been sequenced and then overexpressed in E. coli producing a protein of M(r) approximately. 27,500. Cell-free extracts of the transformed E. coli strain are able to methylate added Mg protoporphyrin, resulting in the formation of Mg protoporphyrin monomethyl ester. The iden...

journal_title：FEBS letters

authors： Gibson LC,Hunter CN

更新日期：1994-09-26 00:00:00

abstract：:The alpha3/beta4 subtype of neuronal nicotinic acetylcholine receptor (nAChR) was stably expressed in human embryonic kidney (HEK) 293 cells that co-expressed a voltage-gated Ca2+ channel. alpha3/beta4-nAChR-expressing clones were identified using the fura-2 Ca2+ imaging technique, and were further characterised by si...

journal_title：FEBS letters

authors： Stetzer E,Ebbinghaus U,Storch A,Poteur L,Schrattenholz A,Kramer G,Methfessel C,Maelicke A

更新日期：1996-11-11 00:00:00

abstract：:Post-transcriptional regulation of gene expression by RNA-binding proteins and by small non-coding RNAs plays an important role in cell biology. Our previous results show that in murine skeletal myoblasts, the expression of Pinch-2, a focal adhesion remodeling factor that regulates cell motility, is repressed by an RN...

journal_title：FEBS letters

authors： Boudoukha S,Rivera Vargas T,Dang I,Kropp J,Cuvellier S,Gautreau A,Polesskaya A

更新日期：2014-05-02 00:00:00

abstract：:The gamma subunit of the F1 moiety of the bovine mitochondrial H(+)-ATP synthase is shown to function as a component of the gate. Addition of purified gamma subunit to F0-liposomes inhibits transmembrane proton conduction. This inhibition can be removed by the bifunctional thiol reagent diamide. Immunoblot analysis sh...

journal_title：FEBS letters

authors： Papa S,Guerrieri F,Zanotti F,Fiermonte M,Capozza G,Jirillo E

更新日期：1990-10-15 00:00:00

abstract：:The biological role of phosphoproteins depends upon their degree of phosphorylation in vivo. Methods currently available to measure the degree of phosphorylation of a protein involve indirect procedures to detect the 32P-phosphate incorporation. We report here a direct method to measure relative amounts of phospho- an...

journal_title：FEBS letters

authors： Di Luca M,de Graan PN,De Angelis L,Gispen WH,Cattabeni F

更新日期：1992-04-20 00:00:00

abstract：:Glycogen storage diseases type 1 (GSD 1) are a group of autosomal recessive disorders characterized by impairment of terminal steps of glycogenolysis and gluconeogenesis. Mutations of the glucose-6-phosphatase gene are responsible for the most frequent form of GSD 1, the subtype 1a, while mutations of the glucose-6-ph...

journal_title：FEBS letters

authors： Galli L,Orrico A,Marcolongo P,Fulceri R,Burchell A,Melis D,Parini R,Gatti R,Lam C,Benedetti A,Sorrentino V

更新日期：1999-10-08 00:00:00

abstract：:Zwittermicin A (ZwA) is a hybrid polyketide-non-ribosomal peptide that is thought to be biosynthesized from five proposed building blocks, including the 2,3-diaminopropionate. Candidate genes for de novo biosynthesis of 2,3-diaminopropionate, zwa5A and zwa5B, have been identified in a previous study. In this research,...

journal_title：FEBS letters

authors： Zhao C,Song C,Luo Y,Yu Z,Sun M

更新日期：2008-09-03 00:00:00

abstract：:A 33-kDa protein component of the oxygen-evolving complex in photosystem II is essential for photosynthesis, and it has been believed that mutants with deletion of this 33-kDa protein are not found in higher plants. We report here the first isolation of an Arabidopsis thaliana mutant with a defect in one of the genes ...

journal_title：FEBS letters

authors： Murakami R,Ifuku K,Takabayashi A,Shikanai T,Endo T,Sato F

更新日期：2002-07-17 00:00:00

abstract：:The host-controlled EcoK restriction of unmodified phage lambda was five-fold alleviated in the wild-type Escherichia coli strain K12 carrying the R64 plasmid of the incompatibility group I1. The relevant gene was mapped between the origin of vegetative replication (rep, oriV) and the tet(r) gene about 60 kbp downstre...

journal_title：FEBS letters

authors： Rastorguev SM,Zavilgelsky GB,Tchurikov NA

更新日期：1998-04-10 00:00:00

abstract：:Rab proteins are small GTPases involved in the regulation of membrane traffic. Rab5a has been shown to regulate transport in the early endocytic pathway. Here we report the isolation of cDNA clones encoding two highly related isoforms, Rab5b and Rab5c. The two proteins share with Rab5a all the structural features requ...

journal_title：FEBS letters

authors： Bucci C,Lütcke A,Steele-Mortimer O,Olkkonen VM,Dupree P,Chiariello M,Bruni CB,Simons K,Zerial M

更新日期：1995-06-05 00:00:00

abstract：:The yeast integral plasma membrane protein Ist2 belongs to a group of membrane proteins which are synthesized from localized mRNAs. The protein reaches the plasma membrane via the ER on a route operating independently of the classical secretory pathway. We have identified a complex peptide-sorting signal located at th...

journal_title：FEBS letters

authors： Franz A,Maass K,Seedorf M

更新日期：2007-02-06 00:00:00

abstract：:Biological functions of transcribed spacer regions in eucaryotic pre-rRNAs remain elusive so far. Utilization of the comparative approach to analyse their secondary structure has been impeded by the extensive sequence divergence observed among most of the specimens available to date. However, we have taken advantage o...

journal_title：FEBS letters

authors： Michot B,Despres L,Bonhomme F,Bachellerie JP

更新日期：1993-02-01 00:00:00