The depolarization response element in acetylcholine receptor genes is a dual-function E box.

abstract：:To unravel the mechanism of interleukin-13 (IL-13)-specific functions, we sought to identify IL-13 receptor (IL-13R) binding molecules. A novel human IL-13Ralpha1 binding protein (IL13RBP1) has been identified using yeast tri-hybrid system, which was found to encode the same protein as MIP-T3 (microtubule interacting ...

journal_title：FEBS letters

authors： Niu Y,Murata T,Watanabe K,Kawakami K,Yoshimura A,Inoue J,Puri RK,Kobayashi N

更新日期：2003-08-28 00:00:00

abstract：:cAMP levels in Paramecium increased dose dependently after a step increase of [Ca] or [Sr] in the incubation, provided K was present. Two mM Ca or Sr tripled cAMP concentrations within 3 s and induced an increase in forward swimming speed. The increase in cAMP formation was strictly dependent on the Donnan ratio [K]: ...

journal_title：FEBS letters

authors： Schultz JE,Grünemund R,von Hirschhausen R,Schönefeld U

更新日期：1984-02-13 00:00:00

abstract：:Screening of hemorphins in extracts of rat lung, brain, heart and spleen was carried out. The threshold for detection of hemorphins was 0.01 nmol for spleen and 0.05 nmol for other tissues. Both the content and the composition of hemorphins differed significantly in the tissues analyzed. Heart and lung extracts were r...

journal_title：FEBS letters

authors： Yatskin ON,Philippova MM,Blishchenko EYu,Karelin AA,Ivanov VT

更新日期：1998-05-29 00:00:00

abstract：:The influence of the esterification of the 3'(2') hydroxyl group of tRNA on the hydrolysis by venom phosphodiesterase (Crotalus adamanteus) was studied. It was shown that this esterification does not change the rate of hydrolysis. At pH 7-8, no significant differences were observed either when the alpha amino group of...

journal_title：FEBS letters

authors： Yot P,Gueguen P,Chapeville F

更新日期：1968-08-01 00:00:00

abstract：:It has previously been suggested that the mammary cell could produce prolactin (PRL). This hypothesis was investigated by incubation with [35S]methionine-cysteine followed by SDS-PAGE, immunoblotting and autoradiography of immunoprecipitated PRL, and by electron microscopic analysis after incubation without or with cy...

journal_title：FEBS letters

authors： Lkhider M,Delpal S,Le Provost F,Ollivier-Bousquet M

更新日期：1997-01-20 00:00:00

abstract：:Yeasts with disruptions in the genes PYC1 and PYC2 encoding the isoenzymes of pyruvate carboxylase cannot grow in a glucose-ammonium medium (Stucka et al. (1991) Mol. Gen. Genet. 229, 307-315). We have isolated a dominant mutation, BPC1-1, that allows growth in this medium of yeasts with interrupted PYC1 and PYC2 gene...

journal_title：FEBS letters

authors： Blázquez MA,Gamo FJ,Gancedo C

更新日期：1995-12-18 00:00:00

abstract：:Human milk cholesterol esterase (bile salt-activated lipase) plays a role in the dietary uptake of triacylglyceride and cholesteryl ester. The activities toward these substrates are mediated through a unique bile salt-activated mechanism. Previously, it has been proposed that a necessary step in this process is prior ...

journal_title：FEBS letters

authors： Loomes KM,Senior HE

更新日期：1997-04-01 00:00:00

abstract：:Iron regulatory protein 2 (IRP2) is a critical switch for cellular and systemic iron homeostasis. In iron-deficient or hypoxic cells, IRP2 binds to mRNAs containing iron responsive elements (IREs) and regulates their expression. Iron promotes proteasomal degradation of IRP2 via the F-box protein FBXL5. Here, we explor...

journal_title：FEBS letters

authors： Hausmann A,Lee J,Pantopoulos K

更新日期：2011-02-18 00:00:00

abstract：:The involvement of Ca(2+) in the response to high Mn(2+), Co(2+), Ni(2+), Cu(2+), Zn(2+), Cd(2+), and Hg(2+) was investigated in Saccharomyces cerevisiae. The yeast cells responded through a sharp increase in cytosolic Ca(2+) when exposed to Cd(2+), and to a lesser extent to Cu(2+), but not to Mn(2+), Co(2+), Ni(2+), ...

journal_title：FEBS letters

authors： Ruta LL,Popa VC,Nicolau I,Danet AF,Iordache V,Neagoe AD,Farcasanu IC

更新日期：2014-08-25 00:00:00

abstract：:Glucose-fructose oxidoreductase (GFOR), a periplasmic protein of Zymomonas mobilis, is synthesized as a precursor polypeptide with a twin-R signal peptide for Sec-independent protein export in bacteria. In higher plant chloroplasts, twin-R signal peptides are specific targeting signals for the Sec-independent delta pH...

journal_title：FEBS letters

authors： Halbig D,Hou B,Freudl R,Sprenger GA,Klösgen RB

更新日期：1999-03-19 00:00:00

abstract：:In this study, we investigated enzymatic deglycosylation of glucoamylase from Aspergillus awamori X 100/D27, a glycoprotein which has two N-linked and about forty short mannose-bearing O-linked sugars per molecule. O-Linked sugars were modified by treatment with alpha-mannosidase and N-linked sugars were removed using...

journal_title：FEBS letters

authors： Neustroev KN,Golubev AM,Firsov LM,Ibatullin FM,Protasevich II,Makarov AA

更新日期：1993-01-25 00:00:00

abstract：:Fluorescence techniques were used to study conformational changes that occur in inactive E. coli 30 S ribosomal subunits during activation by heating in 12 mM Mg2+. Activation is associated with movement of a fluorophore on the 3'-end of 16 S RNA into a less polar environment and towards a probe on the cysteine thiol ...

journal_title：FEBS letters

authors： Odom OW,Stöffler G,Hardesty B

更新日期：1984-07-23 00:00:00

abstract：:The accumulation of protoporphyrin IX (Proto IX) in light-sensitive mutants of Escherichia coli was detected by spectrofluorimetry. Fluorescence emission and excitation spectra were recorded from extracts of bacterial cells. Proto IX clearly accumulated in cells with mutations in the visA (hemH) gene but not in the wi...

journal_title：FEBS letters

authors： Miyamoto K,Nishimura K,Masuda T,Tsuji H,Inokuchi H

更新日期：1992-10-05 00:00:00

abstract：:The effects of temperature on the gamma-aminobutyric acid (GABA) uptake and on the presteady-state and transport-associated currents of the GABA cotransporter, rat gamma-aminobutyric acid transporter 1 (rGAT1), have been studied using heterologous oocyte expression and voltage-clamp. Increasing temperature from 15 to ...

journal_title：FEBS letters

authors： Binda F,Bossi E,Giovannardi S,Forlani G,Peres A

更新日期：2002-02-13 00:00:00

abstract：:Phosphatidylinositol transfer proteins (PITP) are abundant cytosolic proteins originally identified because of their ability to act in vitro as specific transporters of phosphatidylinositol or phosphatidylcholine between membranes. However, the cellular function of mammalian PITP has remained enigmatic till recently. ...

journal_title：FEBS letters

authors： Cockcroft S

更新日期：1997-06-23 00:00:00

abstract：:By applying the 'recognition mask' strategy to 300 mammalian sequences containing NotI sites we demonstrated that 5' ends of genes are highly enriched in NotI sites. A NotI linking clone NL2-252 (D3S1678) containing transferrin receptor (TFRC) gene was used as an initial point for chromosomal jumping. One of the jumpi...

journal_title：FEBS letters

authors： Kashuba VI,Gizatullin RZ,Protopopov AI,Allikmets R,Korolev S,Li J,Boldog F,Tory K,Zabarovska V,Marcsek Z,Sumegi J,Klein G,Zabarovsky ER,Kisselev L

更新日期：1997-12-15 00:00:00

abstract：:Immature Drosophila rhodopsin is N-glycosylated, but undergoes complete deglycosylation during the process of protein maturation. In order to elucidate the site of glycosylation and its role in rhodopsin synthesis, we investigated the in vitro and in vivo synthesis of rhodopsin whose putative N-glycosylation sites (As...

journal_title：FEBS letters

authors： Katanosaka K,Tokunaga F,Kawamura S,Ozaki K

更新日期：1998-03-13 00:00:00

abstract：:Fas/FasL interactions have been proposed as a potentially important mechanism mediating beta-cell death in type 1 diabetes. Recent investigations suggest RNA interference, afforded by small interfering RNAs (siRNA), can provide specific and robust gene silencing in mammalian cells. The current study attempted to inves...

journal_title：FEBS letters

authors： Burkhardt BR,Lyle R,Qian K,Arnold AS,Cheng H,Atkinson MA,Zhang YC

更新日期：2006-01-23 00:00:00

abstract：:In the presence of ammonium sulfate the absorption spectra of a peroxidase from the fungus Arthromyces ramosus (ARP) showed that the low-spin component increased as the pH increased from 6.0 to 9.0, whereas in its absence ARP remained in the high-spin state in the pH range investigated. The crystal structure of ARP at...

journal_title：FEBS letters

authors： Kunishima N,Amada F,Fukuyama K,Kawamoto M,Matsunaga T,Matsubara H

更新日期：1996-01-15 00:00:00

abstract：:2-Deoxyglucose and 5-thioglucose, in the same fashion as glucose, cause the inactivation of the rat hepatocyte glycogen phosphorylase and the activation of glycogen synthase. However, 6-deoxyglucose and 1,5-anhydroglucitol inactivate phosphorylase without increasing the activation state of glycogen synthase. With 3-O-...

journal_title：FEBS letters

authors： Carabaza A,Ciudad CJ,Baqué S,Guinovart JJ

更新日期：1992-01-20 00:00:00

abstract：:Laser flash-induced photovoltage responses of the D85S and D85T mutants as well as of the wild-type acid blue form are similar and reflect intraprotein charge redistribution caused by retinal isomerization. The Cl- -induced transition of all of these blue forms into purple ones is accompanied by the appearance of elec...

journal_title：FEBS letters

authors： Kalaidzidis IV,Kaulen AD

更新日期：1997-12-01 00:00:00

abstract：:Temperature-induced order/disorder transition profiles were obtained from the membranes of intact Gram-negative bacterial cells by FT-IR analysis of the frequency shifts of the acyl chain methylene symmetric stretching band as a monitor. Cells grown at different temperatures yielded distinct transition profiles. At th...

journal_title：FEBS letters

authors： Schultz C,Naumann D

更新日期：1991-12-02 00:00:00

abstract：:Determination of the protonation state of enzymes is a challenging problem in computational biophysics largely due to the vast number of possible protonic configurations. The protonation state dynamics of respiratory complex I was investigated via Monte Carlo and asynchronous dynamics simulations and a novel eigenvect...

journal_title：FEBS letters

authors： Couch V,Stuchebrukhov A

更新日期：2012-03-09 00:00:00

abstract：:The pancreatic ATP-sensitive potassium channels comprise two subunits: SUR1 and Kir6.2. Two SUR1 mutations, A116P and V187D, reduce channel activity causing persistent hyperinsulinemic hypoglycemia of infancy. We investigated whether these mutations cause temperature sensitive misfolding. We show that the processing d...

journal_title：FEBS letters

authors： Yang K,Fang K,Fromondi L,Chan KW

更新日期：2005-08-01 00:00:00

abstract：:MeCP2 is a chromatin-associated protein that is mutated in Rett syndrome. Its methyl-CpG-binding domain interacts with DNA containing methylated cytosine, but other modes of recruitment to the genome have also been proposed. Here, we use in vitro and in vivo assays to investigate the DNA binding specificity of two AT-...

journal_title：FEBS letters

authors： Lyst MJ,Connelly J,Merusi C,Bird A

更新日期：2016-09-01 00:00:00

abstract：:Artificial spider silk proteins may form fibers with exceptional strength and elasticity. Wrapping silk, or aciniform silk, is the toughest of the spider silks, and has a very different protein composition than other spider silks. Here, we present the characterization of an aciniform protein (AcSp1) subunit named W1, ...

journal_title：FEBS letters

authors： Xu L,Tremblay ML,Orrell KE,Leclerc J,Meng Q,Liu XQ,Rainey JK

更新日期：2013-10-01 00:00:00

abstract：:Four 12.2-12.6 kDa small heat-shock proteins (sHSPs) of Caenorhabditis elegans are the smallest known members of the sHSP family. They essentially comprise the characteristic C-terminal 'alpha-crystallin domain' of the sHSPs, having a very short N-terminal region, and lacking a C-terminal tail. Recombinant Hsp12.2 and...

journal_title：FEBS letters

authors： Kokke BP,Leroux MR,Candido EP,Boelens WC,de Jong WW

更新日期：1998-08-21 00:00:00

abstract：:Constitutively secreted proteins have traditionally been believed to be excluded from the regulated secretory pathway. In this work we show that kappa light chain and Fc fragment, two markers of the constitutive pathway, are present in the regulated pathway in AtT-20 cells. They colocalize with the endogenous hormone ...

journal_title：FEBS letters

authors： Castle AM,Huang AY,Castle JD

更新日期：1998-11-20 00:00:00

abstract：:Evidence is presented, by means of both fluorescence and action spectroscopy, that a small, spectroscopically heterogeneous population of both Chl a and Chl b molecules is present in isolated spinach thylakoids and is active in photoinhibition. The broadness of the action spectrum suggests that degraded or incompletel...

journal_title：FEBS letters

authors： Santabarbara S,Neverov KV,Garlaschi FM,Zucchelli G,Jennings RC

更新日期：2001-02-23 00:00:00

abstract：:Crosslinking of proteins by tissue transglutaminase (tTG) is enhanced in amyloid (Abeta) deposits characteristic of Alzheimer's disease and sporadic inclusion body myositis. Small heat shock proteins (sHsps) also occur in amyloid deposits. We here report the substrate characteristics for tTG of six sHsps. Hsp27, Hsp20...

journal_title：FEBS letters

authors： Boros S,Kamps B,Wunderink L,de Bruijn W,de Jong WW,Boelens WC

更新日期：2004-10-08 00:00:00