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Temperature effects on the presteady-state and transport-associated currents of GABA cotransporter rGAT1.

Abstract：

:The effects of temperature on the gamma-aminobutyric acid (GABA) uptake and on the presteady-state and transport-associated currents of the GABA cotransporter, rat gamma-aminobutyric acid transporter 1 (rGAT1), have been studied using heterologous oocyte expression and voltage-clamp. Increasing temperature from 15 to 30 degrees C increased GABA uptake, diminished the maximal value of the relaxation time constant of the presteady-state currents and increased the amplitude of the current associated with the transport of GABA. The curve of the presteady-state charge versus voltage was shifted toward negative potentials by increasing the temperature, while the maximal amount of charge (Q(max)) remained constant; the tau versus V curve was also negatively shifted by increasing temperatures. Analysis of the outward (alpha) and inward (beta) rate constants as functions of temperature showed that they are affected differently, with a Q(10)=3.4 for alpha and Q(10)=1.5 for beta. The different temperature coefficients of the rate constants account for the observed shifts. These observations are consistent with a charge moving mechanism based on a conformational change of the protein; the weaker temperature sensitivity of the inward rate constant suggests a rate-limiting diffusional component on this process.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Binda F,Bossi E,Giovannardi S,Forlani G,Peres A

doi

10.1016/s0014-5793(02)02271-8

keywords:

subject

Has Abstract

pub_date

2002-02-13 00:00:00

pages

303-7

issue

1-3

eissn

0014-5793

issn

1873-3468

pii

S0014579302022718

journal_volume

512

pub_type

杂志文章

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