Abstract:
:Zwittermicin A (ZwA) is a hybrid polyketide-non-ribosomal peptide that is thought to be biosynthesized from five proposed building blocks, including the 2,3-diaminopropionate. Candidate genes for de novo biosynthesis of 2,3-diaminopropionate, zwa5A and zwa5B, have been identified in a previous study. In this research, zwa5A was interrupted and chemically synthesized 2,3-diaminopropionate was used to feed the zwa5A(-) mutant. Results showed that feeding with 2,3-diaminopropionate restored the ability of the zwa5A(-) mutant to produce ZwA. Another non-ribosomal peptide synthase gene, designated orf3, was identified. Amino acid dependent PPi release assay showed that the adenylation domain ZWAA2 of ORF3 acyl-adenylated l-2,3-diaminopropionate effectively. Taken together, it can be concluded that l-2,3-diaminopropionate is indeed one of the building blocks for the biosynthesis of Zwittermicin A.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Zhao C,Song C,Luo Y,Yu Z,Sun Mdoi
10.1016/j.febslet.2008.07.054subject
Has Abstractpub_date
2008-09-03 00:00:00pages
3125-31issue
20eissn
0014-5793issn
1873-3468pii
S0014-5793(08)00661-3journal_volume
582pub_type
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