Weak pulling forces exerted on Nin-orientated transmembrane segments during co-translational insertion into the inner membrane of Escherichia coli.

Abstract:

:Transmembrane helices (TMHs) in membrane proteins can be orientated with their N-terminus towards the cytoplasm (Nin), or facing the non-cytoplasmic side (Nout). Most membrane proteins are inserted co-translationally into membranes, aided by Sec-type translocons. Since the final orientation of Nin- and Nout-orientated TMHs differs, they could also interact differently with the translocon and the surrounding membrane during insertion. We measured pulling forces exerted on Nin-orientated TMHs during co-translational insertion into the inner membrane of Escherichia coli. Our results demonstrate that Nin-orientated TMHs experience a weaker pulling force but retain the overall biphasic force profile seen previously for Nout-orientated TMHs (Ismail et al., 2012 [1]).

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Cymer F,Ismail N,von Heijne G

doi

10.1016/j.febslet.2014.03.050

subject

Has Abstract

pub_date

2014-05-21 00:00:00

pages

1930-4

issue

10

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(14)00269-5

journal_volume

588

pub_type

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