Abstract:
:Transmembrane helices (TMHs) in membrane proteins can be orientated with their N-terminus towards the cytoplasm (Nin), or facing the non-cytoplasmic side (Nout). Most membrane proteins are inserted co-translationally into membranes, aided by Sec-type translocons. Since the final orientation of Nin- and Nout-orientated TMHs differs, they could also interact differently with the translocon and the surrounding membrane during insertion. We measured pulling forces exerted on Nin-orientated TMHs during co-translational insertion into the inner membrane of Escherichia coli. Our results demonstrate that Nin-orientated TMHs experience a weaker pulling force but retain the overall biphasic force profile seen previously for Nout-orientated TMHs (Ismail et al., 2012 [1]).
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Cymer F,Ismail N,von Heijne Gdoi
10.1016/j.febslet.2014.03.050subject
Has Abstractpub_date
2014-05-21 00:00:00pages
1930-4issue
10eissn
0014-5793issn
1873-3468pii
S0014-5793(14)00269-5journal_volume
588pub_type
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