Abstract:
:Structural analogs of adenosine 5'-acylphosphates, which are intermediates of the reaction catalyzed by acyl-CoA synthetases, were synthesized by condensing primary alcohols with AMP to examine the inhibitory effects on the lignoceroyl-CoA and palmitoyl-CoA synthetase activities. Hexadecyl, octadecyl, eicosyl, docosyl and tetracosyl esters of AMP were remarkably potent inhibitors of the lignoceroyl-CoA formation. On the other hand, the eicosyl, docosyl or tetracosyl esters of AMP did not behave as significant inhibitors of the palmitoyl-CoA formation at the concentration at which the two other shorter chain analogs were effective. Namely, these longer alkyl esters of AMP have selective inhibitory effects on the lignoceroyl-CoA synthetase activity. The Ki value of adenosine 5'-tetracosylphosphate, the most potent inhibitor, was about one tenth lower than the Km value for the substrate lignoceric acid. Furthermore, the results support the notion that lignoceroyl-CoA synthetase is distinct from palmitoyl-CoA synthetase.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Shiraishi T,Tezuka K,Uda Ydoi
10.1016/0014-5793(94)00992-9subject
Has Abstractpub_date
1994-10-03 00:00:00pages
353-5issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(94)00992-9journal_volume
352pub_type
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