Abstract:
:The oleosin glycine-rich protein genes Atgrp-6, Atgrp-7, and Atgrp-8 occur in clusters in the Arabidopsis genome and are expressed specifically in the tapetum cells. The cis-regulatory regions involved in the tissue-specific gene expression were investigated by fusing different segments of the gene cluster to the uidA reporter gene. Common distal regulatory regions were identified that coordinate expression of the sequential genes. At least two of these genes were regulated spatially by proximal and distal sequences. The cis-acting elements (122 bp upstream of the transcriptional start point) drive the uidA expression to floral tissues, whereas distal 5' upstream regions restrict the gene activity to tapetal cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Franco LO,de O Manes CL,Hamdi S,Sachetto-Martins G,de Oliveira DEdoi
10.1016/s0014-5793(02)02557-7keywords:
subject
Has Abstractpub_date
2002-04-24 00:00:00pages
13-8issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579302025577journal_volume
517pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Three compounds with cytokinin activity have been isolated from the medium of Rhodospirillum rubrum grown photosynthetically. Two N-6 aminopurine cytokinins revealed in the medium were identical with those obtained from R. rubrum cells previously. The third compound with cytokinin activity in Amaranthus caudatus bioas...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00430-h
更新日期:1995-05-22 00:00:00
abstract::Bisdioxopiperazine anti-cancer agents are catalytic inhibitors of topoisomerase II which by unknown means lock the enzyme in a closed clamp form and inhibit its ATPase activity. In order to demarcate a putative pharmacophore, we here describe a novel Tyr165Ser mutation in the enzyme's Walker A ATP binding site leading...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02805-3
更新日期:2002-06-05 00:00:00
abstract::Functional analysis of heterologously expressed TaTIP2;2 by means of stopped-flow spectrometric studies provide evidence for water and ammonia conductivity. A series of experiments under increasing pH indicate that the gaseous NH(3), rather than the ammonium ion NH(4)(+) was transported. Results from inhibitor studies...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.10.034
更新日期:2007-11-27 00:00:00
abstract::We have expressed the cloned rat kappa-opioid receptor in human embryonic kidney 293 cells and studied the ability of kappa-selective ligands to inhibit adenylyl cyclase. In transfected 293 cells, activation of the kappa-opioid receptor by U50,488 and the dynorphins resulted in the inhibition of cAMP accumulation. The...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00088-q
更新日期:1995-02-20 00:00:00
abstract::The activity of S-adenosylmethionine decarboxylase (AdoMetDC) in Crithidia fasciculata was shown to be correlated to the growth of the parasite. An increase in activity was observed during exponential growth. Inhibition of protein synthesis induced an extremely rapid decay of AdoMetDC activity. The half-life of the en...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00986-4
更新日期:2003-10-09 00:00:00
abstract::In a wild-type strain of Saccharomyces cerevisiae, cadmium induces the activities of both gamma-glutamyl transferase (gamma-GT) and glutathione transferase 2 (Gtt2). However, Gtt2 activity did not increase under gamma-GT or Ycf1 deficiencies, suggesting that the accumulation of glutathione-cadmium in the cytosol inhib...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.03.066
更新日期:2009-05-06 00:00:00
abstract::The nuclear gene OXA1 was first isolated in Saccharomyces cerevisiae and found to be required at a post-translational step in cytochrome c oxidase biogenesis, probably at the level of assembly. Mutations in OXA1 lead to a complete respiratory deficiency. The protein Oxa1p is conserved through evolution and a human hom...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00165-2
更新日期:1996-03-11 00:00:00
abstract::The verprolin family of proteins, WIP, CR16 and WIRE/WICH, has emerged as critical regulators of cytoskeletal organisation in vertebrate cells. The founding father of the family, verprolin, was originally identified in budding yeast and later shown to be needed for actin polymerisation during polarised growth and duri...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2005.08.053
更新日期:2005-10-10 00:00:00
abstract::The effect of the myotoxic drug chlorpromazine was studied in vitro on proteins of sarcoplasmic reticulum and mitochondrial matrix of skeletal muscle in the normal mouse. Our results indicate that the drug is specific for calcium-binding proteins (calcium ATPase, calsequestrin and calmitine). Its proteolytic effect on...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01122-u
更新日期:1995-11-06 00:00:00
abstract::The entire structure of a 98 kb genomic region that abounds in genes related to magnetosome synthesis was first described in the Magnetospirillum sp. strain AMB-1. The deletion of this 98 kb genomic region and the circular form after excision from the chromosome was detected by PCR amplification. This strongly suggest...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.01.003
更新日期:2006-02-06 00:00:00
abstract::Apoptosis repressor with caspase recruitment domain is expressed at high levels in brain and myogenic tissues, consistent with a role to inhibit apoptosis in the terminally differentiated cells. Expression of ARC in cancers is not known. In this study, we reported that ARC was highly expressed in various non-myogenic ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.03.040
更新日期:2005-04-25 00:00:00
abstract::Antibodies generated against synthetic peptides that correspond to highly conserved sequence motifs in the annexins reacted with a variety of annexins from different species. These include Xenopus laevis and Drosophila melanogaster, which contain cross-reacting polypeptides of apparent Mr 34,000 and 30,000. As expecte...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81668-0
更新日期:1989-12-04 00:00:00
abstract::cGMP has been shown to either activate or inhibit Na,K-ATPase activity. Using mouse brain endothelial cells which express both ouabain-resistant alpha1 and ouabain-sensitive alpha2 and alpha3 isoforms, we show that cGMP reduces total Na,KATPase activity to about 58%. The inhibition is prevented by the protein kinase G...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01183-1
更新日期:1998-10-09 00:00:00
abstract::We have identified and purified a DNA binding protein which specifically binds to a segment of the mouse alpha 2 (I) collagen promoter between -420 and -399 bp upstream of the start of transcription. Purification included heparin-agarose and sequence-specific DNA-affinity chromatography, followed by SDS-PAGE and renat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81014-q
更新日期:1993-08-02 00:00:00
abstract::The macromolecular diversity and concentrations in the fluid phase of cytoplasm constitute conditions necessary and sufficient for aqueous phase separation. Consequences of phase separation in cytoplasm, including its 'compartmentation', are inferred from analogies with the physicochemical properties of aqueous two-ph...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(95)00159-7
更新日期:1995-03-20 00:00:00
abstract::Platelet aggregation is accompanied by the tyrosine phosphorylation of several proteins including syk. However, some of these proteins are not identified. Recent studies showed that LAT is a syk substrate and is tyrosine phosphorylated during T cell stimulation. In this study, we demonstrated that LAT is present in pl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01584-1
更新日期:1998-12-28 00:00:00
abstract::Numerous bacterial strains produce surface lectins, commonly in the form of fimbriae that are filamentous assemblies of protein subunits. Among the best characterized of these are the type 1 (mannose specific) fimbrial lectins of Escherichia coli that consist almost exclusively of one class of subunit with a molecular...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(87)80654-3
更新日期:1987-06-15 00:00:00
abstract::The impact of hexose-6-phosphate dehydrogenase (H6PDH) on 11beta-hydroxysteroid dehydrogenase (11beta-HSD) type 1 activity was investigated upon coexpression in HEK-293 cells. Confocal microscopy analysis indicated colocalisation of both enzymes at the lumenal side of the endoplasmic reticulum (ER) membrane. Functiona...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.06.065
更新日期:2004-07-30 00:00:00
abstract::In Gram-positive bacteria the tRNA-dependent T box riboswitch regulates the expression of many amino acid biosynthetic and aminoacyl-tRNA synthetase genes through a transcription attenuation mechanism. The Specifier domain of the T box riboswitch contains the Specifier sequence that is complementary to the tRNA antico...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.09.003
更新日期:2013-11-01 00:00:00
abstract::A cDNA encoding a human liver UDPGT (HLUG 25) transcribed and translated in vitro showed that the encoded protein was synthesized as a precursor and was cleaved and glycosylated when dog pancreatic microsomes were present during translation. The UDPGT cDNA was transiently expressed in mammalian cell culture (COS-7 cel...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80111-5
更新日期:1989-01-30 00:00:00
abstract::S100, a subfamily of the EF-hand type calcium sensing proteins, is implicated in many cellular functions including muscle contractility. Two isoforms, S100A1 and S100B, at 2-10 microM significantly inhibit active tension, stiffness and ATPase of skinned single rabbit psoas muscle fibers at sub-maximal (pCa approximate...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02444-9
更新日期:2001-05-25 00:00:00
abstract::Galanin mediates diverse physiological functions in digestive, endocrine, and central nervous systems through G-protein-coupled receptors. Two galanin receptors have been cloned but the gene structures are unknown. We report genomic and cDNA cloning of the mouse GalR1 galanin receptor and demonstrate that the coding s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00695-9
更新日期:1997-07-14 00:00:00
abstract::The plant hormone cytokinin plays a major role in regulating plant growth and development. Here we generated cytokinin-reduction Arabidopsis plants by overexpressing a heterologous cytokinin oxidase gene DSCKX1 from Dendrobium orchid. These transgenic plants exhibited reduced biomass, rapid root growth, decreased abil...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01259-6
更新日期:2003-12-04 00:00:00
abstract::A recent report of electrical measurements on oriented bacteriorhodopsin in gels [(1986) FEBS Lett. 195, 164 168] concluded that low concentrations of diamines reversed the direction of the proton pump. Calculations are presented which show that in low diamine concentrations, charge displacements of the counterion atm...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80775-5
更新日期:1987-05-25 00:00:00
abstract::Antipsychotic drugs of known antidiarrhoeal and anticalmodulin activity inhibited the cholera-toxin-catalysed ADP-ribosylation of proteins of Mr 37,000, 40,000 and 45,000 (thought to be regulatory components of the adenylate cyclase complex) that was previously shown to occur in plasma membranes from rabbit intestinal...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80444-n
更新日期:1990-10-15 00:00:00
abstract::The effect on the structure of profilin of phosphatidylinositol 4,5-bisphosphate (PIP2) binding was probed by fluorescence and circular dichroism (CD) spectroscopy. Fluorescence of Trp3 and Trp31 of profilin at 292 nm showed a linear decrease in solution emission at 340 nm as PIP2/profilin was increased from 0 to 80:1...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80324-a
更新日期:1992-02-03 00:00:00
abstract::Bovine heart cytochrome-c oxidase was reconstituted in liposomes and the kinetics of cytochrome c oxidation were measured by the polarographic and photometric method under uncoupled conditions in the presence of various polyvalent anions. In order to distinguish between specific and unspecific ionic effects of ATP, th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80018-7
更新日期:1986-10-20 00:00:00
abstract::Toll-like receptors (TLRs) are crucial components of the innate immune system, coupling pathogen recognition to a cellular response. We used the MAPPIT mammalian two-hybrid technique to investigate protein-protein interactions in the early steps in TLR signalling. A partial TLR-adaptor interaction map was constructed ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.01.026
更新日期:2007-02-20 00:00:00
abstract::LncRNAs have a critical role in the regulation of cellular processes such as cancer progression and metastasis. In the present study, we confirmed that TUG1 was overexpressed in bladder cancer tissues and established cell lines. Knockdown of TUG1 inhibited bladder cancer cell metastasis both in vitro and in vivo. Furt...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.08.020
更新日期:2015-10-07 00:00:00
abstract::The trend of the electric field and the value of the electric field flux, sensed by the superoxide substrate in the proximity of the active site, were found to be constant in three highly homologous Cu,Zn superoxide dismutases from ox, pig and sheep, which display large differences in net protein charge and distributi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80681-7
更新日期:1989-06-19 00:00:00