Abstract:
:The impact of hexose-6-phosphate dehydrogenase (H6PDH) on 11beta-hydroxysteroid dehydrogenase (11beta-HSD) type 1 activity was investigated upon coexpression in HEK-293 cells. Confocal microscopy analysis indicated colocalisation of both enzymes at the lumenal side of the endoplasmic reticulum (ER) membrane. Functional analysis in intact cells revealed fivefold stimulation of 11beta-HSD1 oxoreductase activity and sixfold decrease of dehydrogenase activity upon coexpression with H6PDH, without changing kinetic parameters in cell lysates. Thus, H6PDH directly determines the reaction direction of 11beta-HSD1 in intact cells as an oxoreductase without changing intrinsic catalytic properties of 11beta-HSD1 by regenerating NADPH in the ER-lumen.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Atanasov AG,Nashev LG,Schweizer RA,Frick C,Odermatt Adoi
10.1016/j.febslet.2004.06.065keywords:
subject
Has Abstractpub_date
2004-07-30 00:00:00pages
129-33issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579304008270journal_volume
571pub_type
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