A chloroplastic RNA-binding protein is a new member of the PPR family.

Abstract:

:P67, a new protein binding to a specific RNA probe, was purified from radish seedlings [Echeverria, M. and Lahmy, S. (1995) Nucleic Acids Res. 23, 4963-4970]. Amino acid sequence information obtained from P67 microsequencing allowed the isolation of genes encoding P67 in radish and Airabidopsis thaliana. Immunolocalisation experiments in transfected protoplasts demonstrated that this protein is addressed to the chloroplast. The RNA-binding activity of recombinant P67 was found to be similar to that of the native protein. A significant similarity with the maize protein CRP1 [Fisk, D.G., Walker, M.B. and Barkan, A. (1999) EMBO J. 18, 2621-2630] suggests that P67 belongs to the PPR family and could be involved in chloroplast RNA processing.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Lahmy S,Barnèche F,Derancourt J,Filipowicz W,Delseny M,Echeverria M

doi

10.1016/s0014-5793(00)01935-9

keywords:

subject

Has Abstract

pub_date

2000-09-01 00:00:00

pages

255-60

issue

2-3

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(00)01935-9

journal_volume

480

pub_type

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