Abstract:
:We have studied the inhibition by a phosphorothioate oligodeoxynucleotide (17PScap) of cDNA synthesis performed by either avian or murine reverse transcriptase. Three different mechanisms of inhibition were identified: at low concentrations (< 100 nM), the cleavage of the RNA template by the retroviral RNase H at the level of the RNA/17PScap duplex accounted for most of the effect, whereas hybrid-arrested cDNA synthesis by an RNase H-independent mechanism marginally contributed to the inhibition. Both mechanisms were sequence-specific. Above 100 nM, the overall cDNA synthesis was reduced in a non-specific manner.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Boiziau C,Moreau S,Toulmé JJdoi
10.1016/0014-5793(94)80145-2subject
Has Abstractpub_date
1994-03-07 00:00:00pages
236-40issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(94)80145-2journal_volume
340pub_type
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