Abstract:
:Okadaic acid is an inhibitor of the protein Ser/Thr phosphatases PP1 and PP2A, which blocks the activation of extracellular signal-regulated protein kinase 5 (ERK5), a member of the MAP kinase family activated by growth factors and several types of stressors. The blocking of ERK5 activation by okadaic acid was observed in HeLa cells exposed to epidermal growth factor and H(2)O(2) as well as in PC12 cells stimulated by nerve growth factor and H(2)O(2). Calyculin A, another PP1 and PP2A inhibitor, behaved similarly although these compounds are not structurally related. This suggests that either PP1 or PP2A or both are necessary for ERK5 activation. Protein kinase C (PKC) acts as a negative regulator of the ERK5 activation pathway, however our data suggest that the effects of PKC and the phosphatase are unrelated.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Garcia L,Garcia F,Llorens F,Unzeta M,Itarte E,Gómez Ndoi
10.1016/s0014-5793(02)02950-2keywords:
subject
Has Abstractpub_date
2002-07-17 00:00:00pages
90-4issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579302029502journal_volume
523pub_type
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