Abstract:
:The protein kinase C activator, phorbol-12-myristate-13-acetate (PMA), augments the cyclic AMP accumulation induced by forskolin in pheochromocytoma (PC 12) cells with an EC50 value of 14 nM, while having no effect on basal values. At a concentration of 100 nM PMA markedly augmented the magnitude of the forskolin response and, in addition, caused a slight increase in the potency of forskolin. PMA also enhanced the maximal cyclic AMP accumulation produced by 2-chloroadenosine, and caused a slight increase in potency of the adenosine analog. Since PMA mimics the effect of diacylglycerols that form during the turnover of the membrane lipid, phosphatidylinositol, the results suggest an interrelationship between the systems involved in phosphatidylinositol turnover and cyclic AMP generation in PC 12 cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Hollingsworth EB,Ukena D,Daly JWdoi
10.1016/0014-5793(86)80227-7subject
Has Abstractpub_date
1986-02-03 00:00:00pages
131-4issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(86)80227-7journal_volume
196pub_type
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