Abstract:
:The number of synthetic UTP analogues containing methyl groups in different positions of the ribose moiety were tested as substrates for T7 RNA polymerase (T7 RNAP). Two of these compounds (containing substituents in the 5' position) were shown to be weak substrates of T7 RNAP. 3'Me-UTP was neither substrate nor inhibitor of T7 RNAP while 2'Me-UTP was shown to terminate RNA chain synthesis. Conformational analysis of the analogues and parent nucleotide using the force-field method indicates that the allowed conformation of UTP during its incorporation into the growing RNA chain by T7 RNAP is limited to the chi angle range of 192-256 degrees of N-type conformation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tunitskaya VL,Rusakova EE,Padyukova NSh,Ermolinsky BS,Chernyi AA,Kochetkov SN,Lysov YuP,Mikhailov SNdoi
10.1016/s0014-5793(96)01400-7subject
Has Abstractpub_date
1997-01-06 00:00:00pages
263-6issue
3eissn
0014-5793issn
1873-3468pii
S0014579396014007journal_volume
400pub_type
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