N-copine: a novel two C2-domain-containing protein with neuronal activity-regulated expression.

abstract：:An electrometric technique was used to investigate electron transfer between spinach plastocyanin (Pc) and photooxidized primary electron donor P700 in photosystem I (PS I) complexes from the cyanobacterium Synechocystis sp. PCC 6803. In the presence of Pc, the fast unresolvable kinetic phase of membrane potential gen...

journal_title：FEBS letters

authors： Mamedov MD,Mamedova AA,Chamorovsky SK,Semenov AY

更新日期：2001-07-06 00:00:00

abstract：:Patients suffering from Refsum disease have a defect in the alpha-oxidation pathway which results in the accumulation of phytanic acid in plasma and tissues. Our previous studies have shown that phytanic acid is also a substrate for the omega-oxidation pathway. With the use of specific inhibitors we now show that memb...

journal_title：FEBS letters

authors： Komen JC,Wanders RJ

更新日期：2006-07-10 00:00:00

abstract：:The precise interactions between the subunits of Gs (alpha s, beta, gamma) and the plasma membrane remain to be established. If alpha s is associated loosely with the inner membrane, is labile during activation, or is always present to some extent in the cytoplasm, then it should fractionate to the supernatant of a hi...

journal_title：FEBS letters

authors： Roth DA,Urasawa K,Leiber D,Insel PA,Hammond HK

更新日期：1992-01-13 00:00:00

abstract：:In human brain the Abeta peptide is produced mainly by neurons and the overexpression of amyloid precursor protein (APP) that involves an increase in Abeta secretion, has been observed in some areas of the Alzheimer's disease patients brain. We have generated two stably transfected human neuroblastoma lines which over...

journal_title：FEBS letters

authors： Recuero M,Serrano E,Bullido MJ,Valdivieso F

更新日期：2004-07-16 00:00:00

abstract：:The crystal structure of the free form of IF1 from Mycobacterium tuberculosis has been determined at 1.47 A resolution. The structure adopts the expected OB fold and matches the high structural conservation among IF1 orthologues. In order to further explore the function of Mtb-IF1, we built a model of its interaction ...

journal_title：FEBS letters

authors： Hatzopoulos GN,Mueller-Dieckmann J

更新日期：2010-03-05 00:00:00

abstract：:The products of the isiAB operon are a chlorophyll antenna protein (IsiA) and flavodoxin (IsiB), which accumulate in cyanobacteria grown under iron starvation conditions. Here we show that strong light triggers de-repression of isiAB transcription and leads to IsiA and flavodoxin accumulation under iron replete condit...

journal_title：FEBS letters

authors： Havaux M,Guedeney G,Hagemann M,Yeremenko N,Matthijs HC,Jeanjean R

更新日期：2005-04-25 00:00:00

abstract：:Casein phosphopeptides beta-CN(1-25)4P and alpha(s1)-CN(59-79)5P, from beta- and alpha(s1)-casein, respectively, both carrying the characteristic 'acidic motif' Ser(P)-Ser(P)-Ser(P)-Glu-Glu, were chemically synthesized and administered to HT-29 cells differentiated in culture, which are a used model of intestinal epit...

journal_title：FEBS letters

authors： Ferraretto A,Gravaghi C,Fiorilli A,Tettamanti G

更新日期：2003-09-11 00:00:00

abstract：:Human T-cell leukemia and bovine leukemia viruses have a potential transforming gene, termed X. In addition to the major open reading frame known to encode a functional protein, the X gene harbors another short open reading frame which overlaps this major one. Both of these open reading frames are found on a single sp...

journal_title：FEBS letters

authors： Sagata N,Yasunaga T,Ikawa Y

更新日期：1985-11-11 00:00:00

abstract：:The crystal structure of human immunodeficiency virus type 1 (HIV-1) protease mutant G48H with peptidic inhibitor U-89360E is described. Comparison with wild-type protease-inhibitor complex shows that mutation of flap residue 48 to histidine allows stabilizing van der Waals contacts between the side chains of His48 an...

journal_title：FEBS letters

authors： Hong L,Zhang XJ,Foundling S,Hartsuck JA,Tang J

更新日期：1997-12-22 00:00:00

abstract：:Histological studies suggest that adrenal medulla chromaffin cells in situ are polarised, but functional evidence is lacking. We present here the first demonstration for polarisation of exocytosis in isolated, spherical, bovine chromaffin cells. Cells were stimulated with 70 mM K(+) to cause a marked enhancement of ca...

journal_title：FEBS letters

authors： Cuchillo-Ibáñez I,Michelena P,Albillos A,García AG

更新日期：1999-10-01 00:00:00

abstract：:The Escherichia coli signal transduction protein PII, product of the glnB gene, was overproduced and purified. The predicted molecular weight of the protein based on the correct nucleotide sequence is 12,427 and is very close to the value 12,435 obtained by matrix-assisted laser desorption mass spectrometry. Hexagonal...

journal_title：FEBS letters

authors： Vasudevan SG,Gedye C,Dixon NE,Cheah E,Carr PD,Suffolk PM,Jeffrey PD,Ollis DL

更新日期：1994-01-17 00:00:00

abstract：:Several positive transcription factors regulate Arabidopsis anthocyanin biosynthesis. HY5, a component of light-signaling pathways, and PAP1, an R2R3-MYB transcription factor, share common regulatory targets on anthocyanin biosynthesis genes. The epistatic interactions between the two transcription factors are current...

journal_title：FEBS letters

authors： Shin DH,Choi M,Kim K,Bang G,Cho M,Choi SB,Choi G,Park YI

更新日期：2013-05-21 00:00:00

abstract：:Ehrlich ascites tumor cells were found to be in a low bioenergetic status, as evaluated by acridine orange uptake and ATP content, when resuspended in a glucose medium shortly after removal from the animal. Dye uptake as well as ATP content then increased for about 2 h at room temperature. This effect was only slightl...

journal_title：FEBS letters

authors： Dell'Antone P

更新日期：1994-08-22 00:00:00

abstract：:Antibodies generated against synthetic peptides that correspond to highly conserved sequence motifs in the annexins reacted with a variety of annexins from different species. These include Xenopus laevis and Drosophila melanogaster, which contain cross-reacting polypeptides of apparent Mr 34,000 and 30,000. As expecte...

journal_title：FEBS letters

authors： Gerke V

更新日期：1989-12-04 00:00:00

abstract：:The promoter of the cholecystokinin (CCK) gene possesses evolutionary conserved juxtaposed E-box and cAMP/TPA responsive elements (CRE/TRE). We have examined the functional interaction of these two sites. As previously noted, c-Jun/c-Fos heterodimers greatly increase promoter activity through association with the CRE/...

journal_title：FEBS letters

authors： Rourke IJ,Hansen TV,Nerlov C,Rehfeld JF,Nielsen FC

更新日期：1999-04-01 00:00:00

abstract：:Rabbit red blood cells of various ages were separated on Percoll gradients and the activities of two large cytosolic proteases were measured. Both the multicatalytic protease (MCP), assayed by hydrolysis of fluorigenic peptides, and the 26 S ubiquitin/ATP-stimulated protease, assayed by degradation of ubiquitin-lysozy...

journal_title：FEBS letters

authors： Di Cola D,Pratt G,Rechsteiner M

更新日期：1991-03-11 00:00:00

abstract：:DNA polymerase alpha-primase is a heterotetrameric complex essential for simian vacuolating virus 40 (SV40) DNA replication. We show that the C-terminal 67 amino acid residues of the human p180 subunit are essential for SV40 DNA replication as they are required for binding of the p68 subunit and play a role in the int...

journal_title：FEBS letters

authors： Smith RW,Nasheuer HP

更新日期：2002-09-11 00:00:00

abstract：:We present evidence that the potent chloride channel blocker NPPB has protonophoric activity in the mitochondria and across the plasma membrane of phagocytic cells. The resting O2 consumption of murine peritoneal macrophages was stimulated up to 2.5-fold in the presence of NPPB, with a K0.5 of 15 microM. The stimulato...

journal_title：FEBS letters

authors： Lukacs GL,Nanda A,Rotstein OD,Grinstein S

更新日期：1991-08-19 00:00:00

abstract：:The steady-state oxidation of ferrocytochrome c by cytochrome oxidase monitored spectrophotometrically showed that: (1) the kinetics were strictly biphasic with purified enzyme, while mitochondrial membrane-bound enzyme exhibited multiphasic kinetics with extended low affinity phases; (2) the TNmax for the highest aff...

journal_title：FEBS letters

authors： Speck SH,Neu CA,Swanson MS,Margoliash E

更新日期：1983-12-12 00:00:00

abstract：:We explored the interactive effects of endothelial nitric oxide synthase (eNOS) genotypes and cigarette smoking on protein levels and enzyme activity in 33 postpartum placentas. Whilst the eNOS protein levels were lower in the rare allele (0.48+/-0.11, n=9 vs. 1. 05+/-0.10, n=24, P<0.01), the eNOS enzyme activity was ...

journal_title：FEBS letters

authors： Wang XL,Sim AS,Wang MX,Murrell GA,Trudinger B,Wang J

更新日期：2000-04-07 00:00:00

abstract：:We present a new water selective pulse sequence allowing rapid determination of exchange rates of labile protons on the millisecond time scale. Using diffusion measurements, exchange rates of resolved protons can be determined without prior knowledge of relaxation parameters in a short overnight experiment. The use of...

journal_title：FEBS letters

authors： Böckmann A,Guittet E

更新日期：1997-11-24 00:00:00

abstract：:Adiponectin is increasingly recognized as a potential therapeutic agent for the treatment of diabetes and other metabolic diseases. It circulates in plasma as homotrimers and higher-order oliogomers of homotrimers. To facilitate the production of active recombinant adiponectin as a therapeutic tool, we designed a sing...

journal_title：FEBS letters

authors： Min X,Lemon B,Tang J,Liu Q,Zhang R,Walker N,Li Y,Wang Z

更新日期：2012-03-23 00:00:00

abstract：:In tritium-hydrogen exchange experiments, the large GroEL substrate Rubisco was unfolded and exchanged in urea/acid/tritiated water, then diluted into either protic buffer or protic buffer containing GroEL. The respective Rubisco metastable folding intermediate or Rubisco-GroEL binary complex was then separated from r...

journal_title：FEBS letters

authors： Park ES,Fenton WA,Horwich AL

更新日期：2005-02-14 00:00:00

abstract：:Tyrosine-rich acidic matrix protein (TRAMP; 22 kDa extracellular matrix protein; dermatopontin) is a protein that co-purifies with lysyl oxidase and with dermatan sulphate proteoglycans, with possible functions in cell-matrix interactions and matrix assembly. Using a rabbit polyclonal antiserum raised against porcine ...

journal_title：FEBS letters

authors： Forbes EG,Cronshaw AD,MacBeath JR,Hulmes DJ

更新日期：1994-09-12 00:00:00

abstract：:Under physiological conditions significant amounts of low-density lipoprotein LDL particles ar taken up by cells independently of specific high-affinity LDL receptors (apo-B receptors). Previously it was established that some cells contain surface sites capable of binding liposomes. We proposed that liposome-binding s...

journal_title：FEBS letters

authors： Galkina SI,Ivanov VV,Preobrazhensky SN,Margolis LB,Bergelson LD

更新日期：1991-08-05 00:00:00

abstract：:Living isolated mitochondria incubated with iron(II) show a major alteration in mitochondrial DNA (mtDNA) conformational forms as assessed by Southern blot analysis of undigested mtDNA. In the presence of iron(II), form I is transformed into form III in a dose-dependent manner. This alteration in mtDNA conformation sh...

journal_title：FEBS letters

authors： Asín J,Pérez-Martos A,Fernández-Silva P,Montoya J,Andreu AL

更新日期：2000-09-01 00:00:00

abstract：:In Alzheimer disease (AD) the microtubule associated protein (MAP) tau is hyperphosphorylated at several sites. In the present study, like AD tau, tau in the human neuroblastoma SH-SY5Y was found to be hyperphosphorylated, at Ser-199/202, Thr-231, Ser-396 and Ser-404. However, in contrast to AD, the tau in SY5Y cells ...

journal_title：FEBS letters

authors： Tanaka T,Iqbal K,Trenkner E,Liu DJ,Grundke-Iqbal I

更新日期：1995-02-20 00:00:00

abstract：:Tumor necrosis factor receptor associated factor 6 (TRAF6) is an important signaling molecule involved in a diverse array of physiological processes. It has been proposed that TRAF6, a RING finger-containing protein, acts as a ubiquitin ligase (E3) and a target for Lys-63 linked polyubiquitination mediated by Ubc13-Ue...

journal_title：FEBS letters

authors： Wooff J,Pastushok L,Hanna M,Fu Y,Xiao W

更新日期：2004-05-21 00:00:00

abstract：:The serine peptidases, thrombocytin and PA-BJ, isolated from the venom of Bothrops atrox and Bothrops jararaca, respectively, induce platelet aggregation and granule secretion without clotting fibrinogen. The specific platelet aggregation activity of each enzyme was about 15 times lower than that of thrombin. This act...

journal_title：FEBS letters

authors： Santos BF,Serrano SM,Kuliopulos A,Niewiarowski S

更新日期：2000-07-21 00:00:00

abstract：:Stromal cell-derived factor 1alpha (SDF-1alpha) is a chemokine that has been shown to prevent infection of T-tropic HIV strains and is a possible substrate of CD26/dipeptidyl peptidase IV (DPPIV). In this study, we show that SDF-1alpha was cleaved at the N-terminal region by CD26/DPPIV and as a result the inhibitory a...

journal_title：FEBS letters

authors： Ohtsuki T,Hosono O,Kobayashi H,Munakata Y,Souta A,Shioda T,Morimoto C

更新日期：1998-07-17 00:00:00