Abstract:
:Rabbit red blood cells of various ages were separated on Percoll gradients and the activities of two large cytosolic proteases were measured. Both the multicatalytic protease (MCP), assayed by hydrolysis of fluorigenic peptides, and the 26 S ubiquitin/ATP-stimulated protease, assayed by degradation of ubiquitin-lysozyme conjugates, declined 3-fold or less during maturation of rabbit reticulocytes to erythrocytes. The ability of MCP to hydrolyze three classes of peptides decreased in parallel indicating that the 20 S protease is not significantly remodeled during red blood cell maturation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Di Cola D,Pratt G,Rechsteiner Mdoi
10.1016/0014-5793(91)80222-osubject
Has Abstractpub_date
1991-03-11 00:00:00pages
137-40issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(91)80222-Ojournal_volume
280pub_type
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