Phospholipase D treatment enhances gonadotrophin receptor-coupled adenylate cyclase activity in isolated bovine luteal cells.

abstract：:A 15-ns molecular dynamics simulation of the fully hydrated liquid-crystalline dimyristoylphosphatidylcholine-cholesterol (DMPC-Chol) bilayer containing approximately 22 mol% Chol was carried out. The generated trajectory was analysed to investigate the mechanism of the Chol condensing effect on DMPC hydrocarbon chain...

journal_title：FEBS letters

authors： Róg T,Pasenkiewicz-Gierula M

更新日期：2001-07-27 00:00:00

abstract：:The inhibition of electron transport by unnatural (-)-antimycin A3 was examined with rat liver mitochondria and compared with that of natural (+)-antimycin A3. (-)-Antimycin A3 inhibited respiration about 1/100th as strongly as natural (+)-antimycin A3. (-)-Antimycin A3 binding to the cytochrome bc1 complex did not se...

journal_title：FEBS letters

authors： Miyoshi H,Kondo H,Oritani T,Saitoh I,Iwamura H

更新日期：1991-11-04 00:00:00

abstract：:Ribonucleoprotein complexes, which contain mRNAs and their regulator proteins, carry out post-transcriptional control of gene expression. The function of many RNA-binding proteins depends on their association with cofactors. Here, we use a genomic approach to identify transcripts associated with DLC-1, a protein previ...

journal_title：FEBS letters

authors： Day NJ,Ellenbecker M,Voronina E

更新日期：2018-11-01 00:00:00

abstract：:We have identified in rat vascular smooth muscle cells (SMCs) the simultaneous expression of two TGF-beta type I receptor (ALK-5) cDNAs, occurring as a consequence of alternate usage of AG splice acceptor motifs separated by 12 nucleotides located at an intron-exon junction. When translated the resultant full length p...

journal_title：FEBS letters

authors： Agrotis A,Condron M,Bobik A

更新日期：2000-02-04 00:00:00

abstract：:The dinuclear paramagnetic center of the soluble CuA domain of the cytochrome c oxidase from Bacillus subtilis has been studied using 1H NMR. The spectrum possesses remarkably sharp shifted resonances. Comparison with the spectrum of the CuA amicyanin variant provides the spin density distribution in the CuA site of c...

journal_title：FEBS letters

authors： Dennison C,Berg A,de Vries S,Canters GW

更新日期：1996-10-07 00:00:00

abstract：:Expression plasmids encoding native human preporenin and a mutant deleted in its entire prosegment were transfected into Chinese hamster ovary cells. The cells transfected with the expression plasmid of native preporenin secreted exclusively inactive prorenin, while the cells transfected with the mutant secreted the a...

journal_title：FEBS letters

authors： Nagahama M,Nakayama K,Hori H,Murakami K

更新日期：1989-12-18 00:00:00

abstract：:Combining mutation and chemical modification, we have introduced Asp, Gln, Cys, S-carboxymethylcysteine (Cax) and S-carbamoylmethylcysteine (Cam) into the positions of Glu190 and Glu201 of the beta subunit of F1-ATPase from the thermophilic Bacillus PS3. The steady-state ATPase activities of alpha 3 beta 3 gamma compl...

journal_title：FEBS letters

authors： Amano T,Tozawa K,Yoshida M,Murakami H

更新日期：1994-07-04 00:00:00

abstract：:Mod5 is a multifunctional protein that modifies a subset of tRNAs in the cytoplasm and is also required for an RNA-mediated form of transcriptional silencing. Previous in vivo studies have shown that the nuclear silencing function of Mod5 does not require that the causative tRNA gene encode a Mod5 substrate, although ...

journal_title：FEBS letters

authors： Read DF,Waller TJ,Tse E,Southworth DR,Engelke DR,Smaldino PJ

更新日期：2017-06-01 00:00:00

abstract：:We have identified and analyzed a 27-nucleotide sequence (U5 repressive element, designated as U5RE) at the U5 region of the human T-cell leukemia virus type I (HTLV-I) long terminal repeat (LTR) which is required for HTLV-I basal transcriptional repression. The basal promoter strength of constructs that contained del...

journal_title：FEBS letters

authors： Okumura K,Takagi S,Sakaguchi G,Naito K,Minoura-Tada N,Kobayashi H,Mimori T,Hinuma Y,Igarashi H

更新日期：1994-12-12 00:00:00

abstract：:Tropine (tropan-3 alpha-ol) is an intermediate in the formation of hyoscyamine. An acyltransferase activity that can acetylate tropine using acetylcoenzyme A as cosubstrate has been found in transformed root cultures of Datura stramonium. A further acyltransferase activity that acetylates pseudotropine (tropan-3 beta-...

journal_title：FEBS letters

authors： Robins RJ,Bachmann P,Robinson T,Rhodes MJ,Yamada Y

更新日期：1991-11-04 00:00:00

abstract：:The chloroplast inner envelope translocon subunit Tic110 is imported via a soluble stromal translocation intermediate. In this study an in-organellar import system is established which allows for an accumulation of this intermediate in order to analyze its requirements for reexport. All results demonstrate that the re...

journal_title：FEBS letters

authors： Vojta L,Soll J,Bölter B

更新日期：2007-06-12 00:00:00

abstract：:The structural characteristics of the three nuclear phosphoproteins of the high mobility group A family are outlined and related to their participation in chromatin structure alteration in many biological processes such as gene expression, neoplastic transformation, differentiation, and apoptosis. The elevated express...

journal_title：FEBS letters

authors： Sgarra R,Rustighi A,Tessari MA,Di Bernardo J,Altamura S,Fusco A,Manfioletti G,Giancotti V

更新日期：2004-09-10 00:00:00

abstract：:The fungal metabolite brefeldin A (BFA) is known to disrupt the Golgi apparatus resulting in redistribution of Golgi proteins to the endoplasmic reticulum and inhibition of protein secretion. BFA was found to inhibit protein synthesis in rat glioma C6 cells by up to 70% between 0.1 and 1 microgram/ml. Inhibition was b...

journal_title：FEBS letters

authors： Fishman PH,Curran PK

更新日期：1992-12-21 00:00:00

abstract：:Parkinson's disease is the second most common neurodegenerative disease, and results from loss of dopaminergic neurons in the substantia nigra. The aggregation and fibrillation of alpha-synuclein in the form of intracellular proteinaceous aggregates (Lewy bodies and Lewy neurites) have been implicated as a causative f...

journal_title：FEBS letters

authors： Uversky VN,Fink AL

更新日期：2002-07-03 00:00:00

abstract：:Three DNA restriction fragments designated tyrT, 102-mer and 70-mer, have been used as substrates for footprinting studies using DNase I in the presence of the structurally similar antibiotics chartreusin and elsamicin A. The sequence-selective binding sites of the antibiotics can be mapped in regions which are rich i...

journal_title：FEBS letters

authors： Salas X,Portugal J

更新日期：1991-11-04 00:00:00

abstract：:This study was designed to investigate changes in gene expression associated with stage-specific programmed cell death (PCD) in intersegmental muscles (ISMs) of the moth, Manduca sexta. The technique of differential display reverse transcription PCR was applied to compare mRNA levels before and after the onset of PCD ...

journal_title：FEBS letters

authors： Löw P,Talián GC,Sass M

更新日期：2005-09-12 00:00:00

abstract：:L- and D-specific nicotinamide adenine dinucleotide (NAD)-dependent dehydrogenases map to the same structural protein superfamily as defined by the Structural Classification of Proteins (SCOP) and are based on the Rossmann fold type domains. A detailed classification of these domains is proposed using a novel diagnost...

journal_title：FEBS letters

authors： Kutzenko AS,Lamzin VS,Popov VO

更新日期：1998-02-13 00:00:00

abstract：:RCR cells are NRK clones in which Raf-1 production is blocked by the expression of an antisense RNA, and consequently they are refractory to transformation by various oncogenes. In RCR cells, MAP kinases (ERK1 and ERK2) were activated to an extent and in a time course similar to those of the original NRK cells, irresp...

journal_title：FEBS letters

authors： Kizaka-Kondoh S,Okayama H

更新日期：1993-12-27 00:00:00

abstract：:The 4-chlorobenzoyl-CoA dehalogenase catalyzes the hydrolytic dechlorination of 4-chlorobenzoyl-CoA via a two-step mechanism, namely nucleophilic aromatic substitution and ester hydrolysis. The mutation of an active-site Histidine residue has been shown to reduce the catalytic activity in both the substitution and sub...

journal_title：FEBS letters

authors： Xu D,Guo H

更新日期：2005-08-15 00:00:00

abstract：:Atrial natriuretic factor (ANF) and C-type natriuretic peptide (CNP)-activated guanylate cyclases are single-chain transmembrane-spanning proteins, containing both ligand binding and catalytic activities. In both proteins, ligand binding to the extracellular receptor domain activates the cytosolic catalytic domain, ge...

journal_title：FEBS letters

authors： Duda T,Goraczniak RM,Sharma RK

更新日期：1993-12-13 00:00:00

abstract：:The use of bisindolylmaleimide derivatives of staurosporine as selective inhibitors of protein kinase C (PKC) is in doubt following the report by Alessi [FEBS Lett. 402 (1997) 121-123] that Ro31-8220 and GF109203X are potent in vitro inhibitors of p70 S6 kinase and mitogen-activated protein kinase-activated protein ki...

journal_title：FEBS letters

authors： Morreale A,Mallon B,Beale G,Watson J,Rumsby M

更新日期：1997-11-03 00:00:00

abstract：:The quadruple mutation of the whole basic cluster, K74KKK77 conserved in the catalytic subunits of protein kinase CK2 and implicated in substrate recognition, not only abolishes inhibition by heparin but even induces with some peptide substrates an up to 5-fold stimulation by heparin in the 0.5-5 micrograms/ml concent...

journal_title：FEBS letters

authors： Vaglio P,Sarno S,Marin O,Meggio F,Issinger OG,Pinna LA

更新日期：1996-02-12 00:00:00

abstract：:Piriprost and nordihydroguiaretic acid (NDGA), specific inhibitors of arachidonate lipoxygenase, inhibited phytohaemagglutinin (PHA)-stimulated breakdown of inositol lipids in human T lymphocytes. The dual inhibitors eicosatetraynoic acid (ETYA) and BW 755C, which inhibit both lipoxygenase and cyclooxygenase, also had...

journal_title：FEBS letters

authors： Mire-Sluis AR,Cox CA,Hoffbrand AV,Wickremasinghe RG

更新日期：1989-11-20 00:00:00

abstract：:Although signal transducer and activator of transcription 1 (STAT1) mediated regulation of p53 transcription and apoptosis has been previously reported, modulation of other members of the p53 family of transcription factors remains poorly understood. In this study, we found that STAT1 and TA-p73 can interact directly ...

journal_title：FEBS letters

authors： Soond SM,Carroll C,Townsend PA,Sayan E,Melino G,Behrmann I,Knight RA,Latchman DS,Stephanou A

更新日期：2007-03-20 00:00:00

abstract：:We have shown by electrophoretic mobility shift assays that the nucleotide sequence CE-LPH1, centred at position -49 with respect to the transcription start site of the rat gene encoding intestinal lactase-phlorizin hydrolase, interacts in vitro with nuclear proteins present in the jejunum of suckling animals. Protein...

journal_title：FEBS letters

authors： Boukamel R,Freund JN

更新日期：1994-10-10 00:00:00

abstract：:Functional analysis of heterologously expressed TaTIP2;2 by means of stopped-flow spectrometric studies provide evidence for water and ammonia conductivity. A series of experiments under increasing pH indicate that the gaseous NH(3), rather than the ammonium ion NH(4)(+) was transported. Results from inhibitor studies...

journal_title：FEBS letters

authors： Bertl A,Kaldenhoff R

更新日期：2007-11-27 00:00:00

abstract：:Hexaheme nitrite reductases purified to homogeneity from Escherichia coli K-12 and Wolinella succinogenes were studied by low-temperature EPR spectroscopy. In their isolated states, the two enzymes revealed nearly identical EPR spectra when measured at 12 K. Both high-spin and low-spin ferric heme EPR resonances with ...

journal_title：FEBS letters

authors： Liu MC,Liu MY,Payne WJ,Peck HD Jr,Le Gall J,DerVartanian DV

更新日期：1987-06-29 00:00:00

abstract：:The cells responsible for bone formation express protease-activated receptors. Although serine protease thrombin has been shown to elicit functional responses in bone cells that impact on cell survival and alkaline phosphatase activity, nothing is known about tissue factor, factor VIIa, and factor Xa, the serine prote...

journal_title：FEBS letters

authors： Daubie V,De Decker R,Nicaise C,Pochet R

更新日期：2007-06-12 00:00:00

abstract：:Histone deacetylase 6 (HDAC6) is a multi-substrate cytoplasmic enzyme that regulates many important biological processes. Recently, some reports have implicated HDAC6 in viral infection. However, nothing is known about its regulation in virus-infected cells. The data presented here for the first time demonstrate the c...

journal_title：FEBS letters

authors： Husain M,Harrod KS

更新日期：2009-08-06 00:00:00

abstract：:Until cytoplasmic recapping was discovered, decapping was thought to irreversibly destine an mRNA to degradation. Contradicting this idea, we readily observe mRNAs targeted by cytoplasmic capping in uncapped, yet stable forms. 5' rapid amplification of cDNA ends (RACE) shows that nearly all uncapped ends correspond to...

journal_title：FEBS letters

authors： Berger MR,Alvarado R,Kiss DL

更新日期：2019-04-01 00:00:00