Abstract:
:Expression plasmids encoding native human preporenin and a mutant deleted in its entire prosegment were transfected into Chinese hamster ovary cells. The cells transfected with the expression plasmid of native preporenin secreted exclusively inactive prorenin, while the cells transfected with the mutant secreted the active enzyme. The secreted amount of renin from the latter cells was much lower than that of prorenin from the former ones, although these two enzymes had little difference in specific activity after trypsin activation. These results suggest that the prosegment plays an important role in the secretory process of renin, although the fully active enzyme can be formed in its absence.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Nagahama M,Nakayama K,Hori H,Murakami Kdoi
10.1016/0014-5793(89)81528-5subject
Has Abstractpub_date
1989-12-18 00:00:00pages
202-4issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(89)81528-5journal_volume
259pub_type
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