Abstract:
:Free and membrane-bound polyribosomes were isolated from the livers of untreated and clofibrate-treated male C57B1/6 mice. The in vitro translation products were investigated in a rabbit reticulocyte cell-free system by immunoprecipitation of cytosolic epoxide hydrolase, catalase and albumin. The soluble forms of epoxide hydrolase present in cytosol and in peroxisomes were found to be synthesized on free polyribosomes and could not be distinguished from each other, since only one primary translation product was found with the methods used. Clofibrate treatment was found to increase total protein synthesis, synthesis of soluble epoxide hydrolase and translational efficiency of the isolated polyribosomes.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Joste V,Meijer Jdoi
10.1016/0014-5793(89)80021-3subject
Has Abstractpub_date
1989-05-22 00:00:00pages
83-8issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(89)80021-3journal_volume
249pub_type
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