Ro31-8220 inhibits protein kinase C to block the phorbol ester-stimulated release of choline- and ethanolamine-metabolites from C6 glioma cells: p70 S6 kinase and MAPKAP kinase-1beta do not function downstream of PKC in activating PLD.

abstract：:Thrombin is a potent activator of human glomerular epithelial cells (HGEC). Here we compare short-term and long-term effects of thrombin and thrombin receptor agonist peptide (TRAP) which selectively activates the functional thrombin receptor. TRAP, as thrombin, increases intracellular free Ca2+ concentration and acts...

journal_title：FEBS letters

authors： Zacharias U,He CJ,Nguyen G,Sraer JD,Rondeau E

更新日期：1993-11-15 00:00:00

abstract：:Prolonged incubation of membrane fragments containing homogeneous (Na+ + K+)-ATPase with Mg2+, K+ and VO-3 at 4 degrees C resulted in formation of two-dimensional crystals of this enzyme with unit cell parameters: a = 66 A, b = 118 A, gamma = 108 degrees. The crystals correspond to the two-sided plane group p21. By co...

journal_title：FEBS letters

authors： Ovchinnikov YuA,Demin VV,Barnakov AN,Kuzin AP,Lunev AV,Modyanov NN,Dzhandzhugazyan KN

更新日期：1985-10-07 00:00:00

abstract：:Winter flounder contains two distinct anti-freeze protein isoforms, which are the liver-type extracellular anti-freeze proteins and the skin-type intracellular anti-freeze protein. The skin-type anti-freeze proteins exhibit lower anti-freeze activities than the liver-type isoforms and this might be due to their lackin...

journal_title：FEBS letters

authors： Lin Q,Ewart KV,Yang DS,Hew CL

更新日期：1999-06-25 00:00:00

abstract：:The critical urea concentration (C3*) which destabilizes the structure of bovine serum albumin and chymotrypsinogen was determined by UV difference spectroscopy. The increase of the relative content of mobile rotating water molecules in aqueous urea was formerly shown by millimeter spectroscopy [1]. The rise of rotato...

journal_title：FEBS letters

authors： Khurgin Yu,Maksareva E

更新日期：1993-01-04 00:00:00

abstract：:A tetradecapeptide with a sequence identical to residues 26-39 of the cro protein from bacteriophage lambda has been synthesized. This peptide has no secondary structure in an aqueous buffer but adopts an alpha-helical conformation in the presence of 20% hexafluoroisopropanol. The fluorescence of the single tyrosyl re...

journal_title：FEBS letters

authors： Mayer R,Lancelot G,Hélène C

更新日期：1983-03-21 00:00:00

abstract：:By using monoiodinated radioligands of both intact neuropeptide Y (NPY) and of a long C-terminal fragment, NPY13-36, two subtypes of binding sites, which differ in affinity and specificity, have been characterized. The Y1 type of binding site, characterized on a human neuroblastoma cell line, MC-IXC, and a rat pheochr...

journal_title：FEBS letters

authors： Sheikh SP,Håkanson R,Schwartz TW

更新日期：1989-03-13 00:00:00

abstract：:The monoclonal antibody 791T /36, prepared against a human osteogenic sarcoma cell line, 791T , reacts with a variety of human tumours and also mitogen-stimulated PBMN cells. The target antigen as expressed upon 791T cells is a monomeric plasma membrane-associated glycoprotein with an apparent Mr of 72000. By quantita...

journal_title：FEBS letters

authors： Price MR,Campbell DG,Robins RA,Blecher TE,Baldwin RW

更新日期：1984-06-04 00:00:00

abstract：:hTrp3 is a human homologue of the Drosophila gene responsible for a transient receptor potential (trp) mutation. When stably expressed in HEK293 cells, hTrp3 formed ion channels that were active under resting conditions but could be further stimulated by carbachol or ATP via endogenous muscarinic or purinergic recepto...

journal_title：FEBS letters

authors： Hurst RS,Zhu X,Boulay G,Birnbaumer L,Stefani E

更新日期：1998-02-06 00:00:00

abstract：:The flash-induced voltage response of halorhodopsin at high NaCl concentration comprises two main kinetic components. The first component with tau approximately 1 micros does not exceed 4% of the overall response amplitude and is probably associated with the formation of the L (hR520) intermediate. The second main com...

journal_title：FEBS letters

authors： Kalaidzidis IV,Kalaidzidis YL,Kaulen AD

更新日期：1998-05-01 00:00:00

abstract：:Several lines of evidence led to the conclusion that mammalian ribosomal protein synthesis is a highly organized biological process in vivo. A wealth of data support the concept according to which tRNA aminoacylation, formation of the ternary complex on EF1A and delivery of aminoacyl-tRNA to the ribosome is a processi...

journal_title：FEBS letters

authors： Mirande M

更新日期：2010-01-21 00:00:00

abstract：:Insulin resistant states are associated with an increase in the beta cell mass and also high levels of circulating insulin. Ultimately the beta cells undergo a failure that leads to diabetes. At this stage, a question arises if those persistent high levels of circulating insulin may contribute to beta cell damage. To ...

journal_title：FEBS letters

authors： Guillen C,Bartolomé A,Nevado C,Benito M

更新日期：2008-11-26 00:00:00

abstract：:When rat pancreatic islets were incubated with 10(-8) M arginine vasopressin in the presence of 15 mM glucose there was a pronounced inhibition of insulin release in comparison with controls. This inhibitory effect appeared to be specific for vasopressin since it was antagonised by vasopressin antibody. Moreover, pert...

journal_title：FEBS letters

authors： Khalaf LJ,Taylor KW

更新日期：1988-04-11 00:00:00

abstract：:Two clones were isolated by screening a shrimp hepatopancreas cDNA library with a DNA fragment obtained by PCR amplification using two oligonucleotides based on the partial protein sequence of Penaeus vanameii chymotrypsin purified earlier. One of these clones, PVC 7 contains a complete cDNA coding for a serine protea...

journal_title：FEBS letters

authors： Sellos D,Van Wormhoudt A

更新日期：1992-09-14 00:00:00

abstract：:An improved method for expressing and purifying bovine pancreatic ribonuclease from a synthetic gene using the lambda promoter controlled by a temperature-sensitive repressor is described. The procedure involves isolation in the presence of a refolding buffer containing oxidized and reduced glutathione, under conditio...

journal_title：FEBS letters

authors： McGeehan GM,Benner SA

更新日期：1989-04-10 00:00:00

abstract：:Only one copy of the cell adhesion molecule L1 gene is present in the mouse genome, and only one mRNA of 6 kilobases (kb) is expressed in mouse brain [1987, Neurosci. Lett. 82, 89-94]. We have constructed 5 synthetic oligonucleotide probes covering different parts of the published mouse L1 cDNA sequence. Using these p...

journal_title：FEBS letters

authors： Gaardsvoll H,Andersson AM,Bock E

更新日期：1991-12-02 00:00:00

abstract：:A human vesicular monoamine transporter cDNA has been identified by screening a human brainstem library using sequences from the rat brain synaptic vesicle monoamine transporter (SVMT) [(1992) Cell 70, 539-551; (1992) Proc. Natl. Acad. Sci. USA 89, 10993-10997]. The hSVMT shares 92% amino acid identity with the rat se...

journal_title：FEBS letters

authors： Surratt CK,Persico AM,Yang XD,Edgar SR,Bird GS,Hawkins AL,Griffin CA,Li X,Jabs EW,Uhl GR

更新日期：1993-03-08 00:00:00

abstract：:Differences in the metabolism of cancer cells or cancer stem cells (CSCs) as compared to normal cells have provided avenues to safely target cancers. To discover metabolic inhibitors of CSCs, we performed alkaline phosphatase- and tumoursphere-based drug screening using induced cancer stem cell-like cells. From the sc...

journal_title：FEBS letters

authors： Subedi A,Muroi M,Futamura Y,Kawamura T,Aono H,Nishi M,Ryo A,Watanabe N,Osada H

更新日期：2019-04-01 00:00:00

abstract：:Programmed death-ligand 1 (PD-L1) is a co-inhibitory molecule expressed on tumor cells. Immune checkpoint inhibitors focusing on the PD-L1 mechanism are now being studied for the treatment of various cancer types. However, the regulatory mechanism of PD-L1 is yet to be fully clarified, and a high-throughput system for...

journal_title：FEBS letters

authors： Uchida Y,Matsushima T,Kurimoto R,Chiba T,Inutani Y,Asahara H

更新日期：2021-01-09 00:00:00

abstract：:Open reading frame 67 of Bombyx mori nucleopolyhedrovirus (BmORF67) is a homologue of Autographa californica multiple NPV ORF81. The gene is conserved among all baculoviruses and is thus considered a baculovirus core gene. The transcript of BmORF67 was detected at 18-72 h post-infection (p.i.). Polyclonal antiserum ra...

journal_title：FEBS letters

authors： Chen HQ,Chen KP,Yao Q,Guo ZJ,Wang LL

更新日期：2007-12-22 00:00:00

abstract：:DNA polymerase alpha-primase is a heterotetrameric complex essential for simian vacuolating virus 40 (SV40) DNA replication. We show that the C-terminal 67 amino acid residues of the human p180 subunit are essential for SV40 DNA replication as they are required for binding of the p68 subunit and play a role in the int...

journal_title：FEBS letters

authors： Smith RW,Nasheuer HP

更新日期：2002-09-11 00:00:00

abstract：:The oxysterol binding proteins (OSBPs) are believed to control cholesterol homeostasis but their precise mechanism of action is not well understood. The Dictyostelium osbA gene encodes a predicted OSBP, OSBPa, which lacks the PH domain that in most other OSBPs directs targetting to the Golgi. OSBPa instead localises s...

journal_title：FEBS letters

authors： Fukuzawa M,Williams JG

更新日期：2002-09-11 00:00:00

abstract：:Escherichia coli phosphofructokinase-2 (Pfk-2) is a homodimer whose subunits consist of a large domain and an additional beta-sheet that provides the interfacial contacts between the subunits, creating a beta-barrel flattened-like structure with the adjacent subunit's beta-sheet. To determine how the structural organi...

journal_title：FEBS letters

authors： Baez M,Babul J

更新日期：2009-06-18 00:00:00

abstract：:Coagulation factor VII contains ten gamma-carboxyglutamic acid residues in the N-terminal region (Gla-domain) which are essential for the hemostatic function of FVII. The present study shows that granulocyte cathepsin G degrades the Gla-domain of FVIIa in vitro. Characterization of the truncated FVIIa by SDS-PAGE and ...

journal_title：FEBS letters

authors： Nicolaisen EM,Petersen LC,Thim L,Jacobsen JK,Christensen M,Hedner U

更新日期：1992-07-20 00:00:00

abstract：:Recombinant vaccinia virus vectors were used to coexpress each of the candidate prohormone convertases PC1, PC2, furin, PACE4 and PC5 with rat prosomatostatin (rProSOM) in the constitutive secreting cell line LoVo and in the endocrine corticotroph cell line AtT-20, which exhibits regulated secretion. Mammalian ProSOM ...

journal_title：FEBS letters

authors： Brakch N,Galanopoulou AS,Patel YC,Boileau G,Seidah NG

更新日期：1995-04-03 00:00:00

abstract：:Antisera were raised against a phosphoprotein of 65 kDa (PP65) from Paramecium cells (shown before to be selectively dephosphorylated during synchronous exocytosis) and specified by immunoblotting. By immunofluorescence PP65 has been localized within the cortex, beneath the cell membrane. This corresponds to data obta...

journal_title：FEBS letters

authors： Stecher B,Höhne B,Gras U,Momayezi M,Glas-Albrecht R,Plattner H

更新日期：1987-10-19 00:00:00

abstract：:The effective synthesis of the envelope antigen F1 of Y. pestis in E. coli HB101 is mediated by the expression of the caf1M gene. This gene was sequenced, and the protein encoded was found to have a significant homology with the chaperone protein PapD of uropathogenic E. coli. The data presented allow one to suppose C...

journal_title：FEBS letters

authors： Galyov EE,Karlishev AV,Chernovskaya TV,Dolgikh DA,Smirnov OYu,Volkovoy KI,Abramov VM,Zav'yalov VP

更新日期：1991-07-29 00:00:00

abstract：:The plant hormone abscisic acid (ABA) regulates important developmental and stress responses. Recent data show that ABA activates phosphorylation events, but whether dephosphorylation events are post-translationally regulated by ABA or whether these are constitutive remains unknown. Slow anion channels in the plasma m...

journal_title：FEBS letters

authors： Schwarz M,Schroeder JI

更新日期：1998-05-29 00:00:00

abstract：:We have investigated the action of hydrocortisosone on arachidonic acid mobilisation in cultures of mouse peritoneal macrophages, mouse L929 cells and the mouse macrophage-like cell line RAW264. Hydrocortisone inhibits both arachidonic acid release and prostaglandin production by L929 cells. However, prostaglandin pro...

journal_title：FEBS letters

authors： Wood JN,Coote PR,Rhodes J

更新日期：1984-08-20 00:00:00

abstract：:In hepatocytes from control rats, the ureogenic action of epinephrine is mainly mediated through alpha 1-adrenoceptors and the effect is independent of the presence of extracellular calcium. In hepatocytes from adrenalectomized rats, both alpha 1- and beta-adrenoceptors are involved in the action of epinephrine. Furth...

journal_title：FEBS letters

authors： Hernández-Sotomayor SM,García-Sáinz JA

更新日期：1984-01-30 00:00:00

abstract：:The alpha-subunit of the TRAP complex (TRAP alpha) is a single-spanning membrane protein of the endoplasmic reticulum (ER) which is found in proximity of nascent polypeptide chains translocating across the membrane. Here, we demonstrate the widespread occurrence of TRAP alpha in eukaryotes as indicated by its existenc...

journal_title：FEBS letters

authors： Hartmann E,Prehn S

更新日期：1994-08-08 00:00:00