Abstract:
:Tropine (tropan-3 alpha-ol) is an intermediate in the formation of hyoscyamine. An acyltransferase activity that can acetylate tropine using acetylcoenzyme A as cosubstrate has been found in transformed root cultures of Datura stramonium. A further acyltransferase activity that acetylates pseudotropine (tropan-3 beta-ol) with acetyl-coenzyme A is also present. These two activities can be partially resolved by anion-exchange chromatography, some fractions containing only the pseudotropine-utilizing activity. The basic properties of these two enzymes are reported and their roles in forming the observed alkaloid spectrum of D. stramonium roots discussed.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Robins RJ,Bachmann P,Robinson T,Rhodes MJ,Yamada Ydoi
10.1016/0014-5793(91)80887-9subject
Has Abstractpub_date
1991-11-04 00:00:00pages
293-7issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(91)80887-9journal_volume
292pub_type
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